Mutation of a Ubiquitously Expressed Mouse Transmembrane Protein (<i>Tapt1</i>) Causes Specific Skeletal Homeotic Transformations

Howell, Gareth R.; Shindo, Mami; Murray, Stephen A.; Gridley, Thomas; Wilson, Lindsay; Schimenti, John C.

doi:10.1534/genetics.106.065177

Cited by 33 publications

(34 citation statements)

References 29 publications

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“…Occasionally, the pod1 egg cells can be fertilized by the pod1 sperm cells under limited pollination to form arrested zygotes or embryos that are defective in positioning and orientation of the cell plate. In mouse, mutation of TAPT1, the putative homolog of POD1, results in a reversed posterior-anterior axis polarity of the axial skeleton, suggesting a role for TAPT1 in body axis formation (Howell et al, 2007). Similar reversion of apical-basal polarity likely takes place in pod1 embryos in Arabidopsis, as shown earlier.…”

Section: The Role Of Pod1 In Pollen Tube Guidance and Early Embryogensupporting

confidence: 69%

POD1 Regulates Pollen Tube Guidance in Response to Micropylar Female Signaling and Acts in Early Embryo Patterning inArabidopsis

Xue

Jia

et al. 2011

The Plant Cell

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The pollen tube germinates from pollen and, during its migration, it perceives and responds to guidance cues from maternal tissue and from the female gametophyte. The putative female cues have recently been identified, but how the pollen tube responds to these signals remains to be unveiled. In a genetic screen for male determinants of the pollen tube response, we identified the pollen defective in guidance1 (pod1) mutant, in which the pollen tubes fail to target the female gametophyte. POD1 encodes a conserved protein of unknown function and is essential for positioning and orienting the cell division plane during early embryo development. Here, we demonstrate that POD1 is an endoplasmic reticulum (ER) luminal protein involved in ER protein retention. Further analysis shows that POD1 interacts with the Ca2+ binding ER chaperone CALRETICULIN3 (CRT3), a protein in charge of folding of membrane receptors. We propose that POD1 modulates the activity of CRT3 or other ER resident factors to control the folding of proteins, such as membrane proteins in the ER. By this mechanism, POD1 may regulate the pollen tube response to signals from the female tissues during pollen tube guidance and early embryo patterning in Arabidopsis thaliana.

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Section: The Role Of Pod1 In Pollen Tube Guidance and Early Embryogensupporting

confidence: 69%

POD1 Regulates Pollen Tube Guidance in Response to Micropylar Female Signaling and Acts in Early Embryo Patterning inArabidopsis

Xue

Jia

et al. 2011

The Plant Cell

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“…Therefore, these seven miRNAs were considered to be over-expressed [7], [16], [17], [18] in the human gastric antrum region.…”

Section: Resultsmentioning

confidence: 99%

MiRNA Expression Profile for the Human Gastric Antrum Region Using Ultra-Deep Sequencing

et al. 2014

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BackgroundMicroRNAs are small non-coding nucleotide sequences that regulate gene expression. These structures are fundamental to several biological processes, including cell proliferation, development, differentiation and apoptosis. Identifying the expression profile of microRNAs in healthy human gastric antrum mucosa may help elucidate the miRNA regulatory mechanisms of the human stomach.Methodology/Principal FindingsA small RNA library of stomach antrum tissue was sequenced using high-throughput SOLiD sequencing technology. The total read count for the gastric mucosa antrum region was greater than 618,000. After filtering and aligning using with MirBase, 148 mature miRNAs were identified in the gastric antrum tissue, totaling 3,181 quality reads; 63.5% (2,021) of the reads were concentrated in the eight most highly expressed miRNAs (hsa-mir-145, hsa-mir-29a, hsa-mir-29c, hsa-mir-21, hsa-mir-451a, hsa-mir-192, hsa-mir-191 and hsa-mir-148a). RT-PCR validated the expression profiles of seven of these highly expressed miRNAs and confirmed the sequencing results obtained using the SOLiD platform.Conclusions/SignificanceIn comparison with other tissues, the antrum’s expression profile was unique with respect to the most highly expressed miRNAs, suggesting that this expression profile is specific to stomach antrum tissue. The current study provides a starting point for a more comprehensive understanding of the role of miRNAs in the regulation of the molecular processes of the human stomach.

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“…19 In addition, the protein has a conserved domain found in other eukaryotic organisms and mutations of the domain result in homeotic transformations during vertebrate development. 20 Here we present studies showing that resistance to an alternative compound class with a different phenotypic profile is also conferred through mutations in pfcarl . Specifically, we show that treating parasites with sublethal concentrations of MMV007564, a benzimidazolyl piperidine identified from phenotypic screens (previously reported EC 50 = 0.5–0.9 μM) against asexual stages of P. falciparum , 21,22 selects for parasites that have acquired mutations in pfcarl .…”

mentioning

confidence: 95%

Plasmodium falciparum Cyclic Amine Resistance Locus (PfCARL), a Resistance Mechanism for Two Distinct Compound Classes

et al. 2016

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MMV007564 is a novel antimalarial benzimidazolyl piperidine chemotype identified in cellular screens. To identify the genetic determinant of MMV007564 resistance, parasites were cultured in the presence of the compound to generate resistant lines. Whole genome sequencing revealed distinct mutations in the gene named Plasmodium falciparum cyclic amine resistance locus (pfcarl), encoding a conserved protein of unknown function. Mutations in pfcarl are strongly associated with resistance to a structurally unrelated class of compounds, the imidazolopiperazines, including KAF156, currently in clinical trials. Our data demonstrate that pfcarl mutations confer resistance to two distinct compound classes, benzimidazolyl piperidines and imidazolopiperazines. However, MMV007564 and the imidazolopiperazines, KAF156 and GNF179, have different timings of action in the asexual blood stage and different potencies against the liver and sexual blood stages. These data suggest that pfcarl is a multidrug-resistance gene rather than a common target for benzimidazolyl piperidines and imidazolopiperazines.

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Mutation of a Ubiquitously Expressed Mouse Transmembrane Protein (Tapt1) Causes Specific Skeletal Homeotic Transformations

Cited by 33 publications

References 29 publications

POD1 Regulates Pollen Tube Guidance in Response to Micropylar Female Signaling and Acts in Early Embryo Patterning inArabidopsis

POD1 Regulates Pollen Tube Guidance in Response to Micropylar Female Signaling and Acts in Early Embryo Patterning inArabidopsis

MiRNA Expression Profile for the Human Gastric Antrum Region Using Ultra-Deep Sequencing

Plasmodium falciparum Cyclic Amine Resistance Locus (PfCARL), a Resistance Mechanism for Two Distinct Compound Classes

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