Mutation of<i>Drosophila homer</i>Disrupts Control of Locomotor Activity and Behavioral Plasticity

Diagana, Thierry T.; Thomas, Ulrike; Prokopenko, Sergei N.; Xiao, Bo; Worley, Paul F.; Thomas, John B.

doi:10.1523/jneurosci.22-02-00428.2002

Cited by 67 publications

(72 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Male flies react to olfactory, visual, and tactile signals with a complex and strong courtship toward females, and this behavior can be conditioned by previous experience. As compared with wild-type flies, homer R102 mutants were lacking behavioral plasticity and failed to form and/or retain the conditioning by the nonreceptive mated female (7). In addition, homer R102 -mutant flies exhibited higher levels of spontaneous locomotor activity.…”

Section: Animal Behaviormentioning

confidence: 94%

“…A total of 12 Homer cDNAs have been cloned from rat, mouse (5,6), Drosophila (7), and human brains (6). Proteins encoded by these cDNAs share a similar structure and are splice variants of three independent mammalian genes (homer-1, 2, and 3) and of one Drosophila gene (D-homer).…”

Section: The Homer Familymentioning

confidence: 99%

“…To establish the function of Homer proteins in Drosophila locomotor activity and behavioral plasticity, mutant flies were generated (homer R102 ) in which the first two exons and half of the third exon of the homer gene were removed (7). A role for Homer in behavioral plasticity was verified by evaluating the performance of homer R102 -mutant males in a courtshipconditioning assay, an associative learning paradigm in Drosophila (49).…”

Section: Animal Behaviormentioning

confidence: 99%

See 2 more Smart Citations

Homer/Vesl Proteins and Their Roles in CNS Neurons

Ehrengruber

Kato

Inokuchi

et al. 2004

View full text Add to dashboard Cite

Section: Animal Behaviormentioning

confidence: 94%

Section: The Homer Familymentioning

confidence: 99%

Section: Animal Behaviormentioning

confidence: 99%

See 1 more Smart Citation

Homer/Vesl Proteins and Their Roles in CNS Neurons

Ehrengruber

Kato

Inokuchi

et al. 2004

View full text Add to dashboard Cite

“…Drosophila Homer (D. Homer) is highly homologous to mammalian Homer1 proteins [261] and recent behavioral screens for Drosophila mutants revealed that Drosophila lacking Homer (homer R102 ) exhibit increased sensitivity to the acute sedative effects of alcohol and fail to develop normal levels of rapid tolerance upon a subsequent alcohol exposure. In support of an active role for Homer in regulating alcohol sensitivity, pan-neuronal expression of WT Homer reduced their initial sensitivity to acute alcohol and restored the development of rapid tolerance [260].…”

Section: Homers and Alcohol-induced Neuroplasticitymentioning

confidence: 99%

Homers regulate drug-induced neuroplasticity: Implications for addiction

Szumlinski

Ary

Lominac

2008

Biochemical Pharmacology

118

160

View full text Add to dashboard Cite

Drug addiction is a chronic, relapsing disorder, characterized by an uncontrollable motivation to seek and use drugs. Converging clinical and preclinical observations implicate pathologies within the corticolimbic glutamate system in the genetic predisposition to, and the development of, an addicted phenotype. Such observations pose cellular factors regulating glutamate transmission as likely molecular candidates in the etiology of addiction. Members of the Homer family of proteins regulate signal transduction through, and the trafficking of, glutamate receptors, as well as maintain and regulate extracellular glutamate levels in corticolimbic brain regions. This review summarizes the existing data implicating the Homer family of protein in acute behavioral and neurochemical sensitivity to drugs of abuse, the development of drug-induced neuroplasticity, as well as other behavioral and cognitive pathologies associated with an addicted state.

show abstract

“…However, more accurate identification and mapping of novel neuronal circuits has been hampered by the lack of a genetically encoded and phenotypically neutral dendritic marker. Over the years, many such markers have been proposed and several were recently examined (12), namely MAP2 (13,14), Nod::YFP (4, 15-18), Homer::GFP (19), and DSCAM17.1::GFP (20,21). The analysis of these markers reveals that none of them labels the entire somatodendritic field.…”

mentioning

confidence: 99%

Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Nicolaï

Ramaekers

Raemaekers

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

266

227

View full text Add to dashboard Cite

In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. We have developed such a marker and show that it is effective and specific in all neuronal populations tested in the peripheral and central nervous system. The marker, which we name DenMark (Dendritic Marker), is a hybrid protein of the mouse protein ICAM5/Telencephalin and the red fluorescent protein mCherry. We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity.T o discover neuronal circuit architecture, genetic tools that specifically mark the pre-and postsynaptic cells and compartments are necessary. Drosophila is a leading genetic model organism in this regard; however, most neuronal circuits remain unmapped. Of particular note is the lack of a universal, phenotypically neutral, and specific marker of the somatodendritic and postsynaptic compartments. Several molecular differences between dendrites and axons, including the presence of different membrane and cytoskeletal proteins in neuronal subregions, have been identified (1, 2). Drosophila neurons exhibit the major kinds of compartmentalization present in mammalian neurons and the fly has emerged as a powerful system to study the establishment and maintenance of neuronal connections (3, 4). Almost all studies of neuronal circuits in the fly have relied on genetic markers such as CD8::GFP that outline the morphology of entire cells rather than particular subcellular compartments (5), as well as presynaptic markers such as Synaptotagmin, Synaptobrevin, and Bruchpilot GFP fusion proteins (6-11). However, more accurate identification and mapping of novel neuronal circuits has been hampered by the lack of a genetically encoded and phenotypically neutral dendritic marker. Over the years, many such markers have been proposed and several were recently examined (12), namely MAP2 (13, 14), Nod::YFP (4, 15-18), Homer::GFP (19), and DSCAM17.1::GFP (20, 21). The analysis of these markers reveals that none of them labels the entire somatodendritic field. Furthermore, it remains unclear whether the markers tested are neutral with respect to dendritic morphology.Intercellular adhesion molecules (ICAMs) mediate neuronal migration, axon elongation, and fasciculation, synaptogenesis, and synaptic plasticity (22). ICAM5, or Telencephalin, is a 130-kDa type I transmembrane glycoprotein comprising a characteristic extracellular domain, a single transmembrane region, and a short cytoplasmic region (23). The expression of ICAM5 is restricted to the mammalian brain telencephalon (24) but there is no homolog in invertebrates and lower vertebrates. The developmental appearance of ICAM5 parallels the time of dendritic elongation, branching, a...

show abstract

Mutation ofDrosophila homerDisrupts Control of Locomotor Activity and Behavioral Plasticity

Cited by 67 publications

References 53 publications

Homer/Vesl Proteins and Their Roles in CNS Neurons

Homer/Vesl Proteins and Their Roles in CNS Neurons

Homers regulate drug-induced neuroplasticity: Implications for addiction

Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Contact Info

Product

Resources

About