Mutation of rice (Oryza sativa L.) LOX-1/2 near-isogenic lines with ion beam implantation and study of their storability

Song, Maoyong; Wu, Yuejin; Zhang, Ying; Liu, B.M.; Jiang, Junguang; Xu, Xinlan; Zhang, Yu

doi:10.1016/j.nimb.2007.09.035

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…In our study, the downregulation of lipoxygenases, an important enzyme associated with the oxidation of polyunsaturated fatty acids, was observed only in imbibed embryos of aged ST seeds. This is consistent with the findings of previous studies in which higher suppression of lipoxygenase expression enhanced the viability and longevity (Song et al, 2007;Xu et al, 2015). In the early stage of seed aging, oxidative damage results in the massive accumulation of lipid peroxide-derived aldehydes and ketones, especially α, β-unsaturated carbonyls, which generate advanced glycation end products and advanced lipoxidation end products by aggregating with proteins and lipids (Nisarga et al, 2017;Fu et al, 2018).…”

Section: Scavenge Cytotoxic Compounds May Cause Differences In Plateau Phase and Storabilitysupporting

confidence: 92%

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

et al. 2021

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The critical node (CN, 85% germination) of seed viability is an important threshold for seed regeneration decisions after long-term conservation. Dependent on the germplasm, the storage period until CN is reached varies and information on the divergence of the proteomic profiles is limited. Therefore, the study aims to identify key proteins and mechanisms relevant for a long plateau phase and a late CN during artificial seed aging of wheat. Seeds of the storage-tolerant genotype (ST) TRI 23248, and the storage-sensitive genotype (SS) TRI 10230 were exposed to artificial ageing (AA) and extracted embryos of imbibed seeds were analyzed using an iTRAQ-based proteomic technique. ST and SS required AA for 24 and 18 days to reach the CN, respectively. Fifty-seven and 165 differentially abundant proteins (DAPs) were observed in the control and aged groups, respectively. Interestingly, a higher activity in metabolic processes, protein synthesis, transcription, cell growth/division, and signal transduction were already found in imbibed embryos of control ST seeds. After AA, 132 and 64 DAPs were accumulated in imbibed embryos of both aged ST and SS seeds, respectively, which were mainly associated with cell defense, rescue, and metabolism. Moreover, 78 DAPs of ST appeared before CN and were mainly enriched in biological pathways related to the maintenance of redox and carbon homeostasis and they presented a stronger protein translation ability. In contrast, in SS, only 3 DAPs appeared before CN and were enriched only in the structural constituents of the cytoskeleton. In conclusion, a longer span of plateau phase might be obtained in seeds when proteins indicate an intense stress response before CN and include the effective maintenance of cellular homeostasis, and avoidance of excess accumulation of cytotoxic compounds. Although key proteins, inherent factors and the precise regulatory mechanisms need to be further investigated, the found proteins may also have functional potential roles during long-term seed conservation.

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Section: Scavenge Cytotoxic Compounds May Cause Differences In Plateau Phase and Storabilitysupporting

confidence: 92%

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

et al. 2021

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“…Lipid degradation was proposed as one cause of seed deterioration during storage (Takano 1993). Similar to the results from soybean, the negative relationships between LOX activity and seed longevity have also been observed in maize and rice (Li et al 2007;Song et al 2007;Jiang et al 2009;Long et al 2013;Gayen et al 2014;Huang et al 2014). For example, after storing seeds of three maize varieties with and without LOX-1, 2 at 42°C and 84 % relative humidity for a period of 15 days, the variety lacking LOX-1, 2 showed a slight change in germination, but for varieties with LOX-1, 2, an obvious decline in germination was observed, suggesting LOX-1, 2 may be a definitive factor which influences seed life span (Li et al 2007).…”

supporting

confidence: 75%

“…The relationships between LOX activity and seed longevity during AA treatment have been well studied in maize and rice (Li et al 2007;Song et al 2007;Jiang et al 2009;Long et al 2013;Gayen et al 2014;Huang et al 2014). However, there have been few reports about the effect of LOXs on wheat grain deterioration.…”

Section: Discussionmentioning

confidence: 99%

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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In higher plants, lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids and can accumulate to relatively high levels in the seeds. However, high LOX activity has often been found associated with harmful effects on the quality attributes and the longevity of harvested seeds. Here, we report the development and characterization of three homozygous T 5 transgenic lines (designated as LOX RNAi -4, 7 and 9, respectively) with LOX activity specifically reduced in the grains of common wheat. An RNAi hairpin directed under an endosperm-specific promoter was introduced into the genome of the elite wheat variety Longchun 23 using particle bombardment. Each transgenic line (LOX RNAi -4, 7 or 9) derived from separate integration event, which carried more than 20 copies of the transgene. The three LOX RNAi lines and wild-type (WT) Longchun 23 showed a similar level of LOX transcripts in the flag leaves. However, relative to WT control, LOX RNAi -4, 7 and 9 exhibited drastic decreases in the level of LOX transcripts during grain development compared with WT control. The LOX activity levels in the harvested grains of the three transgenic lines were significantly lower than that of WT control under either normal conditions or artificial aging (AA, stored at 40°C and 85 % humidity for 6 or 9 days) treatment. The whiteness values of the flour samples from LOX RNAi -4, 7 and 9 were generally and significantly higher than those of WT control regardless of the grains were stored under normal or AA conditions. Furthermore, the germination rates of LOX RNAi -4, 7 and 9 were substantially higher than those of WT control under AA treatment. Our data demonstrate that grainspecific reduction in LOX activity by RNAi may represent a generally applicable approach for improving the quality traits and longevity of crop seeds.Keywords Lipoxygenase Á RNA interference Á Germination rate Á Whiteness Á Wheat Zhenying Dong and Bo Feng have contributed equally to this work.Electronic supplementary material The online version of this article

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“…The low-energy heavy ion beam irradiation equipment employed in this study was identical to that used by Song et al [21]. The parameters used in the present study were as follows: ion energy of 25 keV; N + ion fluence of 2.5 Â 10 16 ions/cm 2 ; irradiation time of 10 min.…”

Section: Low-energy Heavy Ion Beam Implantationmentioning

confidence: 99%

Mutagenic effects of heavy ion irradiation on rice seeds

Xue¹,

Liu²,

Zhang³

et al. 2012

Nuclear Instruments and Methods in Physics Research Section B:

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Mutation of rice (Oryza sativa L.) LOX-1/2 near-isogenic lines with ion beam implantation and study of their storability

Cited by 15 publications

References 19 publications

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Mutagenic effects of heavy ion irradiation on rice seeds

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