Mutation of the GABA receptor associated with fipronil resistance in the whitebacked planthopper, Sogatella furcifera

Nakao, Takeshi; Naoi, Atsuko; Kawahara, Nobuyuki; Hirase, Kangetsu

doi:10.1016/j.pestbp.2010.03.006

Cited by 61 publications

(52 citation statements)

References 24 publications

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“…20) A fipronil-susceptible strain of S. furcifera is the laboratory strain maintained at the Agrochemical Research Center, Mitsui Chemicals Agro, in Japan. Insects were reared on rice seedlings at 25°C under a 16 : 8 light : dark regime and frozen before RNA was isolated.…”

Section: Insectsmentioning

confidence: 99%

“…20) To obtain the 3′ and 5′ ends of a cDNA encoding SF-RDL, a SMARTer TM RACE cDNA amplification kit (Clontech Laboratories, Inc., Mountain View, CA, USA) was used according to the manufacturer's instructions. The 3′ end of the cDNA was amplified using nested PCR following RT-PCR.…”

Section: Isolation Of Full-length Cdna Clones Encoding Sf-rdlmentioning

confidence: 99%

“…They have developed resistance to fipronil and cause serious damage to the rice in Asia. [16][17][18][19] In a previous study, the A2′N mutation was associated with fipronil resistance and was suggested to be a heterozygous mutation in S. furcifera 20) and L. striatellus. 21) A membrane potential assay showed that the A2′N mutation in the L. striatellus RDL GABA receptor subunit (LS-RDL) confers fipronil resistance in the heterozygous expression of wild-type and A2′N mutant LS-RDL genes.…”

Section: Introductionmentioning

confidence: 96%

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Fipronil resistance in Sogatella furcifera: Molecular cloning and functional expression of wild-type and mutant RDL GABA receptor subunits

et al. 2012

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The whitebacked planthopper Sogatella furcifera is a serious pest of rice that has developed fipronil resistance in Asia. We found a novel R340Q mutation in the cytoplasmic loop between M3 and M4 of the S. furcifera RDL γ-aminobutyric acid (GABA) receptor subunit (SF-RDL). The R340Q mutation was found in clones carrying the A2′N mutation, which was previously implicated in fipronil resistance and suggested to be a heterozygous mutation. To investigate the influence of mutations, Drosophila Mel-2 cells were transfected with wild-type or mutant SF-RDL genes, either individually or together. A membrane potential assay showed that the influence of the A2′N · R340Q double mutation on fipronil resistance was more profound than that of the A2′N single mutation in the heterozygous expression of wild-type and mutant SF-RDL genes.

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Section: Insectsmentioning

confidence: 99%

Section: Isolation Of Full-length Cdna Clones Encoding Sf-rdlmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Fipronil resistance in Sogatella furcifera: Molecular cloning and functional expression of wild-type and mutant RDL GABA receptor subunits

et al. 2012

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“…Ser 301 provides low resistance to the phenylpyrazole fipronil that does not impact the use of this insecticide in the field (9,43,46,47). However, fipronil-resistant strains isolated from two species of planthopper have been shown to contain an Ala to Asn, rather than Ser or Gly, mutation at the equivalent 301 site, found only in the heterozygous state (48,49). The lack of homozygous Asn individuals was proposed to be a result of lethality, supported by the reduced GABA median effective concentration (EC 50 ) observed in electrophysiological data (49).…”

mentioning

confidence: 99%

Gene duplication in the major insecticide target site,Rdl, inDrosophila melanogaster

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Schmidt

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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The Resistance to Dieldrin gene, Rdl, encodes a GABA-gated chloride channel subunit that is targeted by cyclodiene and phenylpyrazole insecticides. The gene was first characterized in Drosophila melanogaster by genetic mapping of resistance to the cyclodiene dieldrin. The 4,000-fold resistance observed was due to a single amino acid replacement, Ala 301 to Ser. The equivalent change was subsequently identified in Rdl orthologs of a large range of resistant insect species. Here, we report identification of a duplication at the Rdl locus in D. melanogaster. The 113-kb duplication contains one WT copy of Rdl and a second copy with two point mutations: an Ala 301 to Ser resistance mutation and Met 360 to Ile replacement. Individuals with this duplication exhibit intermediate dieldrin resistance compared with single copy Ser 301 homozygotes, reduced temperature sensitivity, and altered RNA editing associated with the resistant allele. Ectopic recombination between Roo transposable elements is involved in generating this genomic rearrangement. The duplication phenotypes were confirmed by construction of a transgenic, artificial duplication integrating the 55.7-kb Rdl locus with a Ser 301 change into an Ala 301 background. Gene duplications can contribute significantly to the evolution of insecticide resistance, most commonly by increasing the amount of gene product produced. Here however, duplication of the Rdl target site creates permanent heterozygosity, providing unique potential for adaptive mutations to accrue in one copy, without abolishing the endogenous role of an essential gene.T he single point mutation in the Resistance to dieldrin (Rdl) gene represents one of the most significant cases of target site resistance to an insecticide yet observed. Cyclodiene resistance was reported in 62% of insecticide resistant species in the 1980s, following widespread use of cyclodiene insecticides, including dieldrin, which started in the 1950s (1). The nature of the genetic target, Rdl, was discovered after dieldrin was discontinued because of the widespread evolution of resistance in many species. Rdl was first discovered in Drosophila melanogaster using a positional cloning approach. High homology to human GABA receptors confirmed it was the first insect ligand-gated chloride channel subunit identified (2-4). A point mutation in the chloride channel pore-lining domain, replacing alanine 301 with serine, was present in all resistant D. melanogaster strains (5). This mutation provided 4,000-fold resistance when homozygous and lower levels of resistance in heterozygotes (2, 6). The homologous mutation was subsequently found in a large number of cyclodiene-resistant species from many insect orders (7-9), as well as a glycine replacement at the homologous site in some resistant strains of Drosophila simulans and other species (5, 10, 11).Characterization of deficiency lines and inversions in D. melanogaster showed that Rdl is an essential gene (3). Thus, the Ala 301 to Ser or Gly mutation in Rdl exhibits unique properties, p...

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“…13) Sequence analysis of RDL GABA receptor subunit genes from a fipronil-resistant S. furcifera population collected in Fukuoka Prefecture in 2007 identified the A2′N mutation in the heterozygous state. 14) In addition, a novel R340Q mutation was found in the cytoplasmic loop M3-M4 (Fig. 1).…”

Section: Mechanisms Of Fipronil Resistance In Sogatella Furciferamentioning

confidence: 97%

Study on the structure and insecticide sensitivity of the RDL GABA receptor<sup>#</sup>

Nakao

2015

Journal of Pesticide Science

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In this study, a novel A2′N mutation that confers fipronil resistance was found in the membrane-spanning region M2 of the RDL GABA receptor from fipronil-resistant planthoppers in the heterozygous state. Furthermore, rapid monitoring methods using polymerase chain reaction-restriction fragment polymorphism were employed to detect fipronil-resistant planthoppers carrying the A2′N mutation. It was found that 3-benzamido-N-[4-(perfluoropropan-2-yl)phenyl]benzamides (meta-diamides) comprise a distinct class of RDL GABA receptor antagonists with high insecticidal activity. A membrane potential assay demonstrated that meta-diamides acted on A2′ mutant RDL GABA receptors at the same level as the wild-type RDL GABA receptor. In addition, meta-diamides may act on the M1-M3 intersubunit pocket, whereas cyclodienes and fipronil act on the A2′ residue in the pore formed by M2s. Thus, meta-diamides are expected to be effective against cyclodiene-and fipronil-resistant pests carrying A2′ mutations. Meta-diamides also exhibited high selectivity toward insect RDL GABA receptors.

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Mutation of the GABA receptor associated with fipronil resistance in the whitebacked planthopper, Sogatella furcifera

Cited by 61 publications

References 24 publications

Fipronil resistance in Sogatella furcifera: Molecular cloning and functional expression of wild-type and mutant RDL GABA receptor subunits

Fipronil resistance in Sogatella furcifera: Molecular cloning and functional expression of wild-type and mutant RDL GABA receptor subunits

Gene duplication in the major insecticide target site,Rdl, inDrosophila melanogaster

Study on the structure and insecticide sensitivity of the RDL GABA receptor<sup>#</sup>

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