Mutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in<i>Arabidopsis</i>

Peer, Wendy Ann; Hosein, Fazeeda N.; Bandyopadhyay, Anindita; Makam, Srinivas N.; Otegui, Marisa S.; Lee, Gil-Je; Blakeslee, Joshua J.; Cheng, Yan; Titapiwatanakun, Boosaree; Yakubov, Bahktiyor; Bangari, Bharat; Murphy, Angus S.

doi:10.1105/tpc.108.059634

Cited by 49 publications

(76 citation statements)

References 139 publications

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“…1g). The inhibition of the in vitro pollen tube growth by NPA was apparent only at high concentrations (100 µM) indicating that it might occur through APM1-regulated functions of PIN and ABCB auxin transport proteins, as APM1 is the target at high concentrations of NPA treatment 26,27 . The change in sensitivity in pollen tubes overexpressing auxin transport-related protein PIN8 suggests that it is auxin transport-related effect of NPA that affects pollen tube growth.…”

Section: Pin8 Is Expressed and Functions In The Male Gametophytementioning

confidence: 99%

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

et al. 2012

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Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIn auxin transporter family, PIn8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIn8 in regulating auxin homoeostasis and metabolism. PIn8 co-localizes with PIn5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIn5 and PIn8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIn transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

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Section: Pin8 Is Expressed and Functions In The Male Gametophytementioning

confidence: 99%

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

et al. 2012

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“…7). Similar strong embryonic defects have been recently observed in apm1 loss-of-function Arabidopsis mutants, while pin1pin7 mutants do not exhibit these alterations (Peer et al, 2009), suggesting that the existence of multiple NPA targets should be taken into consideration also in maize.…”

Section: Zmpin1 Patterning During Endosperm Developmentmentioning

confidence: 54%

“…Although NPA effects have been reported for multiple targets besides PIN1 (ABCB1 [Noh et al, 2001], ABCB19 [Noh et al, 2001;Nagashima et al, 2008], and Aminopeptidase M1 [APM1; Peer et al, 2009]), our results allowed us to assess the relationship between auxin gradients and correct seed development.…”

Section: Zmpin1 Patterning During Endosperm Developmentmentioning

confidence: 99%

ZmPIN1-Mediated Auxin Transport Is Related to Cellular Differentiation during Maize Embryogenesis and Endosperm Development

2009

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To study the influence of PINFORMED1 (PIN1)-mediated auxin transport during embryogenesis and endosperm development in monocots, the expression pattern of the three identified ZmPIN1 genes was determined at the transcript level. Localization of the corresponding proteins was also analyzed during maize (Zea mays) kernel development. An anti-indole-3-acetic acid (IAA) monoclonal antibody was used to visualize IAA distribution and correlate the direction of auxin active transport, mediated by ZmPIN1 proteins, with the actual amount of auxin present in maize kernels at different developmental stages. ZmPIN1 genes are expressed in the endosperm soon after double fertilization occurs; however, unlike other tissues, the ZmPIN1 proteins were never polarly localized in the plasma membrane of endosperm cells. ZmPIN1 transcripts and proteins also colocalize in developing embryos, and the ZmPIN1 proteins are polarly localized in the embryo cell plasma membrane from the first developmental stages, indicating the existence of ZmPIN1-mediated auxin fluxes. Auxin distribution visualization indicates that the aleurone, the basal endosperm transfer layer, and the embryo-surrounding region accumulate free auxin, which also has a maximum in the kernel maternal chalaza. During embryogenesis, polar auxin transport always correlates with the differentiation of embryo tissues and the definition of the embryo organs. On the basis of these reports and of the observations on tissue differentiation and IAA distribution in defective endosperm-B18 mutant and in N-1-naphthylphthalamic acid-treated kernels, a model for ZmPIN1-mediated transport of auxin and the related auxin fluxes during maize kernel development is proposed. Common features between this model and the model previously proposed for Arabidopsis (Arabidopsis thaliana) are discussed.

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“…Higher NPA concentrations are thought to block protein trafficking [105,118,119] and could affect auxin transports by interfering with their cycling at the plasma membrane. NPA indeed interferes with PIN1 trafficking after disruption by the lactone antibiotic, brefeldin A [105], as well as in the tir3 mutant lacking the scaffolding protein, BIG [119].…”

Section: Imunophilins Might Mediate Auxin Transporter Sensitivity To Npamentioning

confidence: 99%

Master and servant: Regulation of auxin transporters by FKBPs and cyclophilins

2016

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Plant development and architecture are greatly influenced by the polar distribution of the essential hormone auxin. The directional influx and efflux of auxin from plant cells depends primarily on AUX1/LAX, PIN, and ABCB/PGP/MDR families of auxin transport proteins. The functional analysis of these proteins has progressed rapidly within the last decade thanks to the establishment of heterologous auxin transport systems. Heterologous co-expression allowed also for the testing of protein-protein interactions involved in the regulation of transporters and identified relationships with members of the FK506-Binding Protein (FKBP) and cyclophilin protein families, which are best known in non-plant systems as cellular receptors for the immunosuppressant drugs, FK506 and cyclosporin A, respectively. Current evidence that such interactions affect membrane trafficking, and potentially the activity of auxin transporters is reviewed. We also propose that FKBPs andcyclophilins might integrate the action of auxin transport inhibitors, such as NPA, on members of the ABCB and PIN family, respectively. Finally, we outline open questions that might be useful for further elucidation of the role of immunophilins as regulators (servants) of auxin transporters (masters).

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Mutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects inArabidopsis

Cited by 49 publications

References 139 publications

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

ZmPIN1-Mediated Auxin Transport Is Related to Cellular Differentiation during Maize Embryogenesis and Endosperm Development

Master and servant: Regulation of auxin transporters by FKBPs and cyclophilins

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