Mutation of the transcription factor LEAFY COTYLEDON 2 alters the chemical composition of Arabidopsis seeds, decreasing oil and protein content, while maintaining high levels of starch and sucrose in mature seeds

Angeles-Núñez, Juan Gabriel; Tiessen, Axel

doi:10.1016/j.jplph.2011.05.003

Cited by 51 publications

(37 citation statements)

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“…LEC2 activity up-regulates the expression of oleosin, SSPs, LEC1, FUS3, and ABI3 RNAs (Kroj et al, 2003;Santos-Mendoza et al, 2005;Braybrook et al, 2006;To et al, 2006) and is involved in controlling plant growth regulator activity (Braybrook et al, 2006;Stone et al, 2008) and in regulating soluble sugar and starch pathways (Angeles-Núñez and Tiessen, 2011). A number of LEC2 activities associated with seed gene promoter targets and interactions within the seed regulatory network have been elucidated (Braybrook et al, 2006;SantosMendoza et al, 2008).…”

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Following Vegetative to Embryonic Cellular Changes in Leaves of Arabidopsis Overexpressing LEAFY COTYLEDON2

et al. 2013

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Embryogenesis in flowering plants is controlled by a complex interplay of genetic, biochemical, and physiological regulators. LEAFY COTYLEDON2 (LEC2) is among a small number of key transcriptional regulators that are known to play important roles in controlling major events during the maturation stage of embryogenesis, notably, the synthesis and accumulation of storage reserves. LEC2 overexpression causes vegetative tissues to change their developmental fate to an embryonic state; however, little information exists about the cellular changes that take place. We show that LEC2 alters leaf morphology and anatomy and causes embryogenic structures to form subcellularly in leaves of Arabidopsis (Arabidopsis thaliana). Chloroplasts accumulate more starch, the cytoplasm fills with oil bodies, and lytic vacuoles (LVs) appear smaller in size and accumulate protein deposits. Because LEC2 is responsible for activating the synthesis of seed storage proteins (SSPs) during seed development, SSP accumulation was investigated in leaves. The major Arabidopsis SSP families were shown to accumulate within small leaf vacuoles. By exploiting the developmental and tissue-specific localization of two tonoplast intrinsic protein isoforms, the small leaf vacuoles were identified as protein storage vacuoles (PSVs). Confocal analyses of leaf vacuoles expressing fluorescently labeled tonoplast intrinsic protein isoforms reveal an altered tonoplast morphology resembling an amalgamation of a LV and PSV. Results suggest that as the LV transitions to a PSV, the tonoplast remodels before the large vacuole lumen is replaced by smaller PSVs. Finally, using vegetative and seed markers to monitor the transition, we show that LEC2 induces a reprogramming of leaf development.

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Following Vegetative to Embryonic Cellular Changes in Leaves of Arabidopsis Overexpressing LEAFY COTYLEDON2

et al. 2013

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“…En las semillas de Arabidopsis, el almidón se acumula transitoriamente durante la primera mitad del desarrollo, posteriormente es rápidamente degradado durante la fase tardía del desarrollo de la semilla (Baud et al, 2002;Fait et al, 2006;Angeles-Núñez y Tiessen, 2010, 2011, 2012. En colza se ha propuesto que la acumulación del almidón puede reforzar el estatus de la semilla como órgano-fuente principal antes de la síntesis de compuestos de almacenamiento (aceite) y que éste podría regular la asignación de nutrientes entre los diferentes tejidos de la semilla durante el desarrollo (da Silva et al, 1997).…”

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“…In Arabidopsis seeds, starch accumulates temporarily during the first half of the development, and then is rapidly degraded during the late phase of seed development (Baud et al, 2002;Fait et al, 2006;Ángeles-Núñez and Tiessen, 2010, 2011, 2012. In rapeseed has been proposed that the accumulation of starch can enhance the status of the seed as En la maduración intermedia (15 DDF) se observó que la velocidad de la degradación del almidón disminuyó con respecto al silvestre (Ws) (Figura 3).…”

Section: Discussionmentioning

confidence: 99%

“…Después de esta etapa, el embrión adquiere la arquitectura de base de la planta y el albumen se desarrolla a través de divisiones celulares activas (Baud et al, 2002). Durante la etapa de maduración, los compuestos de almacenamiento son sintetizados y acumulados principalmente en el embrión (Mansfield et al, 1992;Baud et al, 2002;Fait et al, 2006;AngelesNúñez y Tiessen, 2010, 2011, 2012. Finalmente en la fase de la maduración tardía, el embrión es metabólicamente inactivo y adquiere tolerancia a la desecación.…”

Section: Introductionunclassified

“…After this stage, the embryo acquires the basic architecture of the plants and the albumen is developed through active cell divisions (Baud et al, 2002). During the maturation stage, storage compounds are synthesized and accumulated mainly in the embryo (Mansfield et al, 1992;Baud et al, 2002;Fait et al, 2006;Ángeles-Núñez and Tiessen, 2010, 2011, 2012. Finally in the late maturation phase, the embryo becomes metabolically inactive and desiccation tolerance.…”

Section: Introductionmentioning

confidence: 99%

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Importancia de la isoforma-1 de la sacarosa sintasa en el metabolismo del carbono durante el desarrollo de la semilla de Arabidopsis thaliana

Angeles-Núñez

Herrera-Corredor

Martínez-Martínez

2018

Remexca

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ResumenLa sacarosa sintasa (SUS) es una enzima clave en el metabolismo de la sacarosa. Esta enzima cataliza la conversión reversible de sacarosa y UDP a UDP-Glucosa y fructosa. En Arabidopsis, la familia de genes SUS consta de 6 miembros. Uno de estos, el gen SUS1 (At5g20830) se expresa durante la fase de desarrollo de la morfogénesis de la semilla, periodo que corresponde a la fase de la acumulación masiva de almidón y de hexosas, a la fecha se desconoce la función metabólica que tiene esta isoforma-1 en la síntesis de estos compuestos carbonados. Con la finalidad de aportar elementos que contribuyan a determinar las funciones de esta enzima (SUS1) en el desarrollo de la semilla, se llevó a cabo un análisis funcional, el cual comprende la caracterización bioquímica de un alelo nulo de esta isoforma. Los resultados obtenidos muestran la importancia de esta enzima (SUS1) para el metabolismo de la sacarosa en la semilla de Arabidopsis en la etapa de desarrollo. Por otro lado, la mutación SUS1 afecta principalmente el cociente hexosas/ sacarosa; lo cual provoca una disminución importante en la acumulación de ácidos grasos y de almidón transitorio durante los estadios tempranos del desarrollo de la semilla.Palabras clave: almidón, ácidos grasos, isoforma-1 de sacarosa sintasa, semillas en desarrollo. AbstractSucrose synthase (SUS) is a key enzyme in the metabolism of sucrose. This enzyme catalyzes the reversible conversion of sucrose and UDP to UDP-glucose and fructose. In Arabidopsis, the family SUS of genes consists of 6 members. One of these, the SUS1 gene (At5g20830) is expressed during the development phase of the morphogenesis of the seed, which corresponds to the phase of the massive accumulation of starch and hexoses to date unknown metabolic function has this isoform-1 in the synthesis of these carbon compounds. In order to provide elements that contribute to determine the roles of this enzyme (SUS1) in seed development was carried out a functional analysis, which comprises the biochemical characterization of a null allele of this isoform. The results show the importance of this enzyme (SUS1) for sucrose metabolism in Arabidopsis seeds in the development stage. Moreover, the mutation affects mainly SUS1 hexoses/sucrose ratio; which results in a significant accumulation of fatty acids and of transitory starch in the early stages of seed development decreased.

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AGC protein kinase AGC1-4 mediates seed size in Arabidopsis

et al. 2020

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Mutation of the transcription factor LEAFY COTYLEDON 2 alters the chemical composition of Arabidopsis seeds, decreasing oil and protein content, while maintaining high levels of starch and sucrose in mature seeds

Cited by 51 publications

References 47 publications

Following Vegetative to Embryonic Cellular Changes in Leaves of Arabidopsis Overexpressing LEAFY COTYLEDON2

Following Vegetative to Embryonic Cellular Changes in Leaves of Arabidopsis Overexpressing LEAFY COTYLEDON2

Importancia de la isoforma-1 de la sacarosa sintasa en el metabolismo del carbono durante el desarrollo de la semilla de Arabidopsis thaliana

AGC protein kinase AGC1-4 mediates seed size in Arabidopsis

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