Mutation spectrum of copper-induced DNA damage.

Tkeshelashvili, L.K.; McBride, Timothy J.; Spence, Kevin; Loeb, Lawrence A.

doi:10.1016/s0021-9258(18)38132-8

Cited by 175 publications

(28 citation statements)

References 37 publications

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“…These discrepancies between Gram-positive and Gram-negative bacteria could be linked to the differences in their cell wall/membrane structures. DNA damages induced by exposition to copper ions are thought to occur mainly through the oxidative action of H 2 O 2 , O 2 •− and OH • [48]. However, in Gram-negative bacteria, the copper-catalyzed formation of OH • takes place in the periplasm.…”

Section: Dna Damagesmentioning

confidence: 99%

Brass Alloys: Copper-Bottomed Solutions against Hospital-Acquired Infections?

Dauvergne

Mullié

2021

Antibiotics

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Copper has been used for its antimicrobial properties since Antiquity. Nowadays, touch surfaces made of copper-based alloys such as brasses are used in healthcare settings in an attempt to reduce the bioburden and limit environmental transmission of nosocomial pathogens. After a brief history of brass uses, the various mechanisms that are thought to be at the basis of brass antimicrobial action will be described. Evidence shows that direct contact with the surface as well as cupric and cuprous ions arising from brass surfaces are instrumental in the antimicrobial effectiveness. These copper ions can lead to oxidative stress, membrane alterations, protein malfunctions, and/or DNA damages. Laboratory studies back up a broad spectrum of activity of brass surfaces on bacteria with the possible exception of bacteria in their sporulated form. Various parameters influencing the antimicrobial activity such as relative humidity, temperature, wet/dry inoculation or wear have been identified, making it mandatory to standardize antibacterial testing. Field trials using brass and copper surfaces consistently report reductions in the bacterial bioburden but, evidence is still sparse as to a significant impact on hospital acquired infections. Further work is also needed to assess the long-term effects of chemical/physical wear on their antimicrobial effectiveness.

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Section: Dna Damagesmentioning

confidence: 99%

Brass Alloys: Copper-Bottomed Solutions against Hospital-Acquired Infections?

Dauvergne

Mullié

2021

Antibiotics

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“…Frequently observed point mutations produced by oxidizing agents (19,20) and ionizing radiation (21)(22)(23)(24), especially in free DNA, are C -T transitions implicating cytosine as the target. The major oxidative product of cytosine is cytosine glycol which is unstable, leading to the formation of uracil glycol, 5-hydroxycytosine, and 5-hydroxyuracil (3,25).…”

Section: Introductionmentioning

confidence: 99%

5-Hydroxypyrimidine deoxynucleoside triphosphates are more efficiently incorporated into DNA by exonuclease-free Klenow fragment than 8-oxopurine deoxynucleoside triphosphates

1994

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Recent studies with 8-oxodeoxyguanosine triphosphate (8-oxodGTP) have suggested that incorporation of oxidized nucleotides from the precursor pool into DNA may have deleterious effects. Here we show that 5-hydroxydeoxycytosine triphosphate (5-OHdCTP) and 5-hydroxydeoxyuridine triphosphate (5-OHdUTP) are more efficient substrates than 8-oxodGTP for Escherichia coli DNA polymerase I Klenow fragment lacking proofreading activity, while 8-oxodeoxyadenosine triphosphate (8-oxodGTP, 5-OHdCTP can mispair with dA in DNA but with lower efficiency. Since the 5-hydroxypyrimidines are present in normal and oxidized cellular DNA in amounts similar to the 8-oxopurines, these data suggest that enzymatic mechanisms might exist for removing them from the DNA precursor pools.

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“…54 Remarkably, Cu + and Cu 2+ were equally mutagenic and ROS scavengers were not fully able to reverse copper mediated DNA damage suggesting that copper can promote DNA damage directly as well. 75 In support of this notion, Oikawa et al revealed that copper predominantly affects guanine bases via a direct interaction. 76 Due to the DNA damaging nature of copper it would be expected that copper would potently induce p53 function and expression.…”

Section: Coppermentioning

confidence: 95%

“…Iron is also an essential transition metal and a major constituent of heme and iron-sulphur proteins that are required for a large number of biological processes, including respiration. 81 Iron exposure predominantly generates DNA damage through the production of ROS, and in particular • OH 75 and iron overload was demonstrated to create single strand breaks in p53 DNA in colon cells. 82 Excess iron is often observed in cancers and iron chelation has been shown to have potential as a therapeutic.…”

Section: Ironmentioning

confidence: 99%

Metal toxicity and the p53 protein: an intimate relationship

Phatak

Müller

2015

Toxicol. Res.

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Many metals are pivotal for the function of enzymes and proteins, but in excess can result in the formation of free radicals (ROS) that can cause damage to DNA. p53 is a tumour suppressor protein and transcription factor that can react to DNA damage to either facilitate a repair response or when the damage is too severe to initiate apoptosis and cell death.ROS can therefore activate p53, but the p53 protein itself is susceptible to oxidative changes, resulting in unfolding of the p53 protein and therefore loss of activity. In order to be active, p53 needs to bind zinc while other metals such as copper can displace zinc leading to p53 unfolding. Metal exposure can therefore result in the activation or inactivation of p53 directly and indirectly through ROS, all dependent on the type of metal and the severity of the exposure. Conversely, p53 itself can impact on ROS and affect the expression of important proteins in metal cell biology. In the current review, we have summarized the relationship between p53, ROS and metal toxicity.

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Mutation spectrum of copper-induced DNA damage.

Cited by 175 publications

References 37 publications

Brass Alloys: Copper-Bottomed Solutions against Hospital-Acquired Infections?

Brass Alloys: Copper-Bottomed Solutions against Hospital-Acquired Infections?

5-Hydroxypyrimidine deoxynucleoside triphosphates are more efficiently incorporated into DNA by exonuclease-free Klenow fragment than 8-oxopurine deoxynucleoside triphosphates

Metal toxicity and the p53 protein: an intimate relationship

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