Mutational analysis of human p53 gene in human gliomas by CleavaseR fragment length polymorphism

Abstract

human p53, 7 EGFR, 8 FGFR2, 9 MDM2, 10 NF2, 11 p16 12 and PTEN/MMAC1 13 genes were reported.For genetic mutation detection, single-strand conformation polymorphism (SSCP) has been widely used recently but requires some special equipment and strict maintenance of reactive temperature. Cleavase ® fragment length polymorphism (CFLP) was developed recently to eliminate the disadvantages of SSCP. The procedure is able to be finished within 1 day and does not require special equipment. It also has more advantages such as the fact that the pattern of objective electrophoresis roughly defines a mutative position of a subjective DNA. [14][15][16][17][18] Therefore, in an attempt to analyze genetic mutations present in human glioma cells, we have employed the CFLP method. The principal aim of this study was to evaluate the efficiency and accuracy of CFLP for mutation detection of human glioma tissues. We screened the p53 gene mutation by CFLP and compared this with results from direct sequence analysis.