Mutational analysis of <i>Arabidopsis thaliana</i> ABCE2 identifies important motifs for its RNA silencing suppressor function

Mõttus, Jelena; Maiste, Siim; Eek, Priit; Truve, Erkki; Sarmiento, Cecilia

doi:10.1111/plb.13193

Cited by 11 publications

(18 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Arabidopsis thaliana (hereafter referred to as Arabidopsis), however, has two ABCE paralogs named ABCE1 and ABCE2 ( Sánchez-Fernández et al., 2001 ; Verrier et al., 2008 ). Arabidopsis ABCE2 has been studied for its RNA silencing suppression activity ( Sarmiento et al., 2006 ; Mõttus et al., 2020 ). In Cardamine hirsuta , a close relative of Arabidopsis with compound leaves, only one ABCE gene has been identified, SIMPLE LEAF3 ( SIL3 ), which is required for leaflet formation and leaf development.…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

Navarro-Quiles¹,

Mateo-Bonmatí²,

Candela³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

ATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored in plants. In most species, ABCE is encoded by a single-copy gene; by contrast, Arabidopsis thaliana has two ABCE paralogs, of which ABCE2 seems to conserve the ancestral function. We isolated apiculata7-1 (api7-1), the first viable, hypomorphic allele of ABCE2, which has a pleiotropic morphological phenotype reminiscent of mutations affecting ribosome biogenesis factors and ribosomal proteins. We also studied api7-2, a null, recessive lethal allele of ABCE2. Co-immunoprecipitation experiments showed that ABCE2 physically interacts with components of the translation machinery. An RNA-seq study of the api7-1 mutant showed increased responses to iron and sulfur starvation. We also found increased transcript levels of genes related to auxin signaling and metabolism. Our results support for the first time a conserved role for ABCE proteins in translation in plants, as previously shown for the animal, fungal, and archaeal lineages. In Arabidopsis, the ABCE2 protein seems important for general growth and vascular development, likely due to an indirect effect through auxin metabolism.

show abstract

Section: Introductionmentioning

confidence: 99%

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

Navarro-Quiles¹,

Mateo-Bonmatí²,

Candela³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Yeast ABCE, as part of the translational apparatus, plays a role in ribosome biogenesis and reactivation for translation regulation [ 58 ]. AtABCE1 and AtABCE2 are involved in RNA interference (RNAi) regulation [ 60 , 61 , 62 ]. Furthermore, the plant ABCFs also participate in translation regulation [ 63 ] and DNA reparation [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of ATP-Binding Cassette Transporters in Chlamydomonas reinhardtii

Yang

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

Microalgae are promising microorganisms used to produce value-added products or to develop sustainable approaches for environmental remediation. The ATP-binding cassette proteins (ABCs) of Chlamydomonas reinhardtii have been characterized as indispensable transporters for CO2 concentrating mechanism, lipid biosynthesis, and heavy metal sequestration. However, few microalgal ABC proteins have been studied compared with higher plants or non-photosynthetic microorganisms. This study performed a genome-wide, evolutionary, and transcriptomic survey of C. reinhardtii ABC proteins (CrABCs). A total of 75 CrABCs were identified and classed into eight ABC subfamilies, from ABCA to ABCI. We found that no whole or partial genome duplication events occurred in C. reinhardtii after the ancient endosymbiosis events, but gene duplications occurred in a small range of chromosomal regions, which forced ABC family expansion. Abundant light, abscisic acid, and jasmonic acid response cis-elements were mapped in the CrABC promoters, coinciding with the evolutionary history of hormone signaling in Chlorophyta. The expression survey under light/dark rhythms revealed a close bond of CrABCs with cell division and development. A broad study of CrABCs supported their expected roles in heavy metal detoxiﬁcation, lipid metabolism, and environmental adaptation. Moreover, the evolutionary and expression survey predicted the functions of unknown CrABCs, which are elaborated in the text. Two half-size CrABCGs—CrABCG3 and CrABCG26—were described as plasma-membrane transporters that might participate in lipidic compound secretion. This study provides fundamental and exhaustive information about CrABCs, which are indispensable for the functional elucidation of ABC proteins in microalgae.

show abstract

“…In this manner, our results strongly suggest that Arabidopsis and, by extension, all plant ABCEs, probably dissociate cytoplasmic ribosomes, as has been reported for species of other kingdoms (Nürenberg-Goloub & Tampé, 2019). In addition, previous works also support a conserved role for the Arabidopsis ABCE2 and human ABCE1 proteins as suppressors of RNA silencing (Braz et al ., 2004; Sarmiento et al, 2006;Kärblane et al ., 2015; Mõttus et al ., 2020). However, we did not find any ABCE2 interactor potentially involved in this process, nor any enriched ontology term related to gene silencing in our RNA-seq assay.…”

Section: Discussionmentioning

confidence: 99%

“…Arabidopsis ABCE2 has been studied for its RNA silencing suppression activity (Sarmiento et al, 2006;Mõttus et al, 2020). In Cardamine hirsuta, a close relative of Arabidopsis with compound leaves, only one ABCE gene has been identified, SIMPLE LEAF3 (SIL3), which is required for leaflet formation and leaf development.…”

Section: Introductionmentioning

confidence: 99%

“…Arabidopsis thaliana (hereafter referred to as Arabidopsis), however, has two ABCE paralogs named ABCE1 and ABCE2 (Sánchez-Fernández et al ., 2001; Verrier et al ., 2008). Arabidopsis ABCE2 has been studied for its RNA silencing suppression activity (Sarmiento et al ., 2006; Mõttus et al ., 2020). In Cardamine hirsuta , a close relative of Arabidopsis with compound leaves, only one ABCE gene has been identified, SIMPLE LEAF3 (SIL3) , which is required for leaflet formation and leaf development.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

Navarro-Quiles

Mateo-Bonmatí

Candela

et al. 2022

Preprint

View full text Add to dashboard Cite

ATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored in plants. In most species, ABCE is encoded by a single-copy gene; by contrast, Arabidopsis thaliana has two ABCE paralogs, of which ABCE2 seems to conserve the ancestral function. We isolated apiculata7-1 (api7-1), a viable, hypomorphic allele of ABCE2, which has a pleiotropic morphological phenotype reminiscent of mutations affecting ribosome biogenesis factors and ribosomal proteins. We also studied api7-2, a null, recessive lethal allele of ABCE2. Co-immunoprecipitation experiments showed that ABCE2 physically interacts with components of the translation machinery. An RNA-seq study of the api7-1 mutant showed increased responses to iron and sulfur starvation. We also found increased transcript levels of genes related to auxin signaling and metabolism. Our results support a conserved role for ABCE proteins in ribosome recycling in plants, as previously shown for the animal, fungal, and archaeal lineages. In plants, the ABCE2 protein seems important for general growth and vascular development, likely due to an indirect effect through auxin metabolism.

show abstract

Mutational analysis of Arabidopsis thaliana ABCE2 identifies important motifs for its RNA silencing suppressor function

Cited by 11 publications

References 63 publications

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

Identification and Characterization of ATP-Binding Cassette Transporters in Chlamydomonas reinhardtii

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

Contact Info

Product

Resources

About