Mutational analysis of the <i>adcCBA</i> genes in <i>Streptococcus gordonii</i> biofilm formation

Mitrakul, Kemthong; Loo, Ching-Yee; Gyurko, Csilla; Hughes, Christopher V.; Ganeshkumar, N.

doi:10.1111/j.1399-302x.2004.00205.x

Cited by 16 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, it has been previously reported that the genes in the S. gordonii adcCBA operon (SGO_1936, SGO_1937, and SGO_1938, respectively) (19,27), which are essential for manganese homeostasis and in vitro biofilm formation, are involved in competence, since strains defective in these genes showed lower transformation frequencies. However, the present microarray analysis suggests that this may not be a direct effect of these genes in response to CSP, since neither the adcCBA gene cluster nor those genes adjacent to them were up-regulated at any of the time points in our studies.…”

Section: S Gordonii Transformation Frequencies In Response To Cspmentioning

confidence: 69%

Genome-Wide Transcriptional Changes inStreptococcus gordoniiin Response to Competence Signaling Peptide

Iobst²,

et al. 2007

View full text Add to dashboard Cite

Streptococcus gordonii is a primary colonizer of the multispecies biofilm on tooth surfaces forming dental plaque and a potential agent of endocarditis. The recent completion of the genome sequence of the naturally competent strain Challis allowed the design of a spotted oligonucleotide microarray to examine a genome-wide response of this organism to environmental stimuli such as signal peptides. Based on temporal responses to synthetic competence signaling peptide (CSP) as indicated by transformation frequencies, the S. gordonii transcriptome was analyzed at various time points after CSP exposure. Microarray analysis identified 35 candidate early genes and 127 candidate late genes that were up-regulated at 5 and 15 min, respectively; these genes were often grouped in clusters. Results supported published findings on S. gordonii competence, showing up-regulation of 12 of 16 genes that have been reported to affect transformation frequencies in this species. Comparison of CSP-induced S. gordonii transcriptomes to results published for Streptococcus pneumoniae strains identified both conserved and species-specific genes. Putative intergenic regulatory sites, such as the conserved combox sequence thought to be a binding site for competence sigma factor, were found preceding S. gordonii late responsive genes. In contrast, S. gordonii early CSP-responsive genes were not preceded by the direct repeats found in S. pneumoniae. These studies provide the first insights into a genome-wide transcriptional response of an oral commensal organism. They offer an extensive analysis of transcriptional changes that accompany competence in S. gordonii and form a basis for future intra- and interspecies comparative analyses of this ecologically important phenotype.

show abstract

Section: S Gordonii Transformation Frequencies In Response To Cspmentioning

confidence: 69%

Genome-Wide Transcriptional Changes inStreptococcus gordoniiin Response to Competence Signaling Peptide

Iobst²,

et al. 2007

View full text Add to dashboard Cite

show abstract

“…We report here the identification of five genes required for biofilm formation in S. sanguinis: adcA, pyrE, purB, thrB, and purL. To our knowledge, adcA is the only one of these to have been previously reported as required for biofilm formation in any bacterium (45). Although these results should be confirmed by complementation analyses, the isolation of two strains each with mutations in the same genes (purB and purL) and the shared involvement of these two genes and pyrE in nucleotide biosynthesis lend support to the involvement of these genes in biofilm formation.…”

Section: Discussionmentioning

confidence: 90%

Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence

et al. 2008

View full text Add to dashboard Cite

Streptococcus sanguinis is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque. S. sanguinis is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of S. sanguinis. Four genes that have not previously been associated with biofilm formation in any other bacterium, purB, purL, thrB, and pyrE, were putatively identified as contributing to in vitro biofilm formation in S. sanguinis. By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in S. sanguinis.

show abstract

“…AdcAII is unique in that it is regulated by the same metalloregulatory protein that controls the expression of the zinc transporter AdcCBA, AdcR (Figure 2; Section 3.8.2) 125,126. Zn(II) binds to SBP domain of AdcAII in a tetrahedral coordination geometry formed by three histidines and one glutamic acid,120 a coordination sphere that is conserved among known Zn(II)-selective SBPs 121,123.…”

Section: Acquisition and Efflux Of Transition Metal Ions In Bacteriamentioning

confidence: 99%

Coordination Chemistry of Bacterial Metal Transport and Sensing

2009

View full text Add to dashboard Cite

She earned her M.S. degree in Chemistry in 2004 and her Ph.D. degree in Chemistry in 2007. Her Ph.D. thesis work, under the guidance of Professor Seth M. Cohen, consisted of examining novel zinc-specific chelators for incorporation into inhibitors of matrix metalloproteinases. In 2008 she started her NIH-funded postdoctoral research with Professor David P. Giedroc, studying zinc homeostasis in Streptococcus pneumoniae. David Giedroc graduated from the Pennsylvania State University in 1980 with his B.S. degree in Biochemistry. After a brief stint in Joseph Villafranca's group at Penn State, he earned his Ph.D. degree in Biochemistry at Vanderbilt University in 1984, where he worked with David Puett on the calcium sensor calmodulin. From 1984 to 1988, he was an NIH postdoctoral fellow in the late Joseph E. Coleman's laboratory at Yale University, where he worked on zinc-finger DNA binding proteins. From 1988 to 2007, he was a member of the faculty in the Department of Biochemistry and Biophysics at Texas A&M University. He is now Professor of Chemistry at Indiana University, where he continues his studies of metalloregulatory proteins and viral RNA structure, folding, and function.

show abstract

Mutational analysis of the adcCBA genes in Streptococcus gordonii biofilm formation

Cited by 16 publications

References 30 publications

Genome-Wide Transcriptional Changes inStreptococcus gordoniiin Response to Competence Signaling Peptide

Genome-Wide Transcriptional Changes inStreptococcus gordoniiin Response to Competence Signaling Peptide

Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence

Coordination Chemistry of Bacterial Metal Transport and Sensing

Contact Info

Product

Resources

About