2006
DOI: 10.1128/jb.00480-06
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Mutational Analysis of the Promoter Recognized by Chlamydia and Escherichia coli σ 28 RNA Polymerase

Abstract: 28 RNA polymerase is an alternative RNA polymerase that has been postulated to have a role in developmental gene regulation in Chlamydia. Although a consensus bacterial 28 promoter sequence has been proposed, it is based on a relatively small number of defined promoters, and the promoter structure has not been systematically analyzed. To evaluate the sequence of the 28 -dependent promoter, we performed a comprehensive mutational analysis of the Chlamydia trachomatis hctB promoter, testing the effect of point s… Show more

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Cited by 29 publications
(48 citation statements)
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“…The frequency matrix was based on a set of 21 known bacterial 28 promoters and takes into account the frequency of occurrence of each nucleotide at each position within this promoter set. The activity matrix used functional data in the form of the relative promoter strength attributable to each nucleotide at each promoter position and was derived from a comprehensive mutational analysis of a 28 promoter (25). For each promoter position, the algorithm assigned a probability value to the four possible nucleotides, with a total probability of 1.…”
Section: Development Of a Program For Extracting Sequencesmentioning
confidence: 99%
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“…The frequency matrix was based on a set of 21 known bacterial 28 promoters and takes into account the frequency of occurrence of each nucleotide at each position within this promoter set. The activity matrix used functional data in the form of the relative promoter strength attributable to each nucleotide at each promoter position and was derived from a comprehensive mutational analysis of a 28 promoter (25). For each promoter position, the algorithm assigned a probability value to the four possible nucleotides, with a total probability of 1.…”
Section: Development Of a Program For Extracting Sequencesmentioning
confidence: 99%
“…C. trachomatis serovar L2 His 6 -28 and E. coli His 6 -28 were individually overexpressed in E. coli BL21(DE3) and purified, as previously described (24,25), to a concentration of 35.7 g/ml and 115.8 g/ml, respectively.…”
Section: Development Of a Program For Extracting Sequencesmentioning
confidence: 99%
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“…CT441 is predicted to be a 28 -regulated gene (26), suggesting that its expression is developmental cycle dependent. To this end, HeLa 229 cells were infected with Chlamydia LGV2 at a multiplicity of infection of 1 IFU/cell for various times, and CT441 (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Functional evidence has been demonstrated for several σ 66 promoters and one σ 28 promoter using mutational analysis and in vitro transcription assays (e.g. Ricci et al, 1993;Fahr et al, 1995;Douglas and Hatch, 1996;Tan et al, 1996;Tan et al, 1998;Ochiai et al, 1999;Schaumburg and Tan, 2003;Yu et al, 2003;Yu et al, 2006). Chlamydial promoters share homology with each of the eubacterial consensus sequences with certain exceptions, including the presence of a cisacting, A/T spacer region in the majority of σ 66 promoters analyzed (Schaumburg and Tan, 2000).…”
Section: Chlamydial Gene Regulationmentioning
confidence: 99%