Mutational and hyperexpression-induced disruption of bipolar budding in yeast

Freedman, Toby; Porter, A. C.; Haarer, Brian K.

doi:10.1099/00221287-146-11-2833

Cited by 8 publications

(4 citation statements)

References 59 publications

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“…The major functional category of Tos8 targets are involved in bud growth including several genes important for bud site selection and bud emergence. A multicopy plasmid containing the TOS8 gene was observed to enhance the random budding pattern of a bud3⌬ mutant in haploids (Freedman et al 2000). Whereas the Tos8 bud site selection target genes are implicated in diploid site selection, their misregulation may influence haploid budding.…”

Section: Functional Specificity Of Transcription Factorsmentioning

confidence: 99%

Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae

Horak¹,

Luscombe²,

Qian³

et al. 2002

Genes Dev.

245

196

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In the yeast Saccharomyces cerevisiae, SBF (Swi4-Swi6 cell cycle box binding factor) and MBF (MluI binding factor) are the major transcription factors regulating the START of the cell cycle, a time just before DNA replication, bud growth initiation, and spindle pole body (SPB) duplication. These two factors bind to the promoters of 235 genes, but bind less than a quarter of the promoters upstream of genes with peak transcript levels at the G1 phase of the cell cycle. Several functional categories, which are known to be crucial for G1/S events, such as SPB duplication/migration and DNA synthesis, are under-represented in the list of SBF and MBF gene targets. SBF binds the promoters of several other transcription factors, including HCM1, PLM2, POG1, TOS4, TOS8, TYE7, YAP5, YHP1, and YOX1. Here, we demonstrate that these factors are targets of SBF using an independent assay. To further elucidate the transcriptional circuitry that regulates the G1-to-S-phase progression, these factors were epitope-tagged and their binding targets were identified by chIp-chip analysis. These factors bind the promoters of genes with roles in G1/S events including DNA replication, bud growth, and spindle pole complex formation, as well as the general activities of mitochondrial function, transcription, and protein synthesis. Although functional overlap exists between these factors and MBF and SBF, each of these factors has distinct functional roles. Most of these factors bind the promoters of other transcription factors known to be cell cycle regulated or known to be important for cell cycle progression and differentiation processes indicating that a complex network of transcription factors coordinates the diverse activities that initiate a new cell cycle.[Keywords: Yeast; transcription; chIp-chip; cell cycle; SBF; MBF] Supplemental material is available at http://www.genesdev.org.

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Section: Functional Specificity Of Transcription Factorsmentioning

confidence: 99%

Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae

Horak¹,

Luscombe²,

Qian³

et al. 2002

Genes Dev.

245

196

View full text Add to dashboard Cite

show abstract

“…The map shows that Msl1 binds Atc1 according to a high-throughput two-hybrid assay, which is named Aip Three Complex 1 for its ability to interact, by two-hybrid assay, with the protein Aip3, also known as Bud6 (Freedman et al, 2000;Amberg and Haarer, SUNY Upstate, personal communication, 2007). We will refer to Aip3/Bud6 as Bud6 for simplicity.…”

Section: Linking Lte1 Regulation With Microtubule-cortex Interactionsmentioning

confidence: 99%

A Novel Pathway that Coordinates Mitotic Exit with Spindle Position

Nelson

Cooper

2007

MBoC

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In budding yeast, the spindle position checkpoint (SPC) delays mitotic exit until the mitotic spindle moves into the neck between the mother and bud. This checkpoint works by inhibiting the mitotic exit network (MEN), a signaling cascade initiated and controlled by Tem1, a small GTPase. Tem1 is regulated by a putative guanine exchange factor, Lte1, but the function and regulation of Lte1 remains poorly understood. Here, we identify novel components of the checkpoint that operate upstream of Lte1. We present genetic evidence in agreement with existing biochemical evidence for the molecular mechanism of a pathway that links microtubule-cortex interactions with Lte1 and mitotic exit. Each component of this pathway is required for the spindle position checkpoint to delay mitotic exit until the spindle is positioned correctly.

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“…These stresses include low nutrient concentration or low availability of assimilable nitrogen [36]. Under stress, yeasts do not undergo axial or bipolar budding but instead exhibit the distal unipolar budding pattern [37]. Unipolar budding involves cells budding only at the pole directly opposite the initial bud scar.…”

Section: Biological Backgroundmentioning

confidence: 99%

An off-lattice discrete model to characterise filamentous yeast colony morphology

Li,

Green,

Tronnolone

et al. 2024

Preprint

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We combine an off-lattice agent-based mathematical model and experimentation to explore filamentous growth of a yeast colony. Under environmental stress,Saccharomyces cerevisiaeyeast cells can transition from a bipolar (sated) to unipolar (pseudohyphal) budding mechanism, where cells elongate and bud end-to-end. This budding asymmetry yields spatially non-uniform growth, where filaments extend away from the colony centre, foraging for food. We use approximate Bayesian computation to quantify how individual cell budding mechanisms give rise to spatial patterns observed in experiments. We apply this method of parameter inference to experimental images of colonies of two strains ofS. cerevisiae, in low and high nutrient environments. The initial nutrient concentration and a forking mechanism for pseudohyphal cell proliferation are the key features driving colony morphology. Simulations run with the most likely inferred parameters produce colony morphologies that closely resemble experimental results.

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Mutational and hyperexpression-induced disruption of bipolar budding in yeast

Cited by 8 publications

References 59 publications

Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae

Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae

A Novel Pathway that Coordinates Mitotic Exit with Spindle Position

An off-lattice discrete model to characterise filamentous yeast colony morphology

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