Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of the brown egg 4 (b-4) mutant in the silkworm, Bombyx mori

Tomihara, Kenta; Satta, Katsuya; Matsuzaki, Shinya; Yoshitake, Kazutoshi; Yamamoto, Kimiko; Uchiyama, Hiroo; Yajima, Seishi; Futahashi, Ryo; Katsuma, Susumu; Osanai-Futahashi, Mizuko; Kiuchi, Takashi

doi:10.1016/j.ibmb.2021.103624

Cited by 3 publications

(3 citation statements)

References 68 publications

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“…The nondiapause eggs produced using the method can be used for the genetic engineering of diapause strains. Construction of transgenic silkworms and genome editing by CRISPR/ Cas9 using low-temperature incubation of the parent eggs was reported, and the method worked well (Zhao et al 2012, Iizuka 2016, Tomihara et al 2021). However, this method is only applicable to some bivoltine strains.…”

Section: Discussionmentioning

confidence: 99%

Development of an Injection Method for the Genetic Engineering of Diapause Silkworm Egg Using Dimethyl Sulfoxide

Yamada

Mise

Yonemura

et al. 2023

JARQ

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Genetic engineering of the domesticated silkworm Bombyx mori is a useful tool for developing sericulture. New races that produce recombinant silk with fluorescent color and spider silk protein have been constructed. Strains resistant to B. mori nuclear polyhedrosis virus have also been constructed and started to rear in the field. In this genetic engineering method, the use of nondiapause eggs that do not arrest embryonic development is indispensable as it includes the injection process of vector DNA into the eggs. However, most silkworm strains are bivoltine or univoltine and lay diapause eggs that arrest their embryonic development. Thus, applying this method to these silkworm strains is impossible. To enable the application, whether dimethyl sulfoxide (DMSO), which can prevent the diapause of eggs, can be used to develop an injection method for the genetic engineering of diapause strains was investigated. The DMSO treatment worked well for this purpose, and an injection method for the genetic engineering of diapause eggs was successfully developed. This method gave high hatchability in the injection of diapause eggs. Genetically modified silkworms were efficiently created in three different diapause silkworms: hybrid race and Japanese and Chinese strains. Thus, DMSO treatment is useful for creating genetically modified silkworms.

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Section: Discussionmentioning

confidence: 99%

Development of an Injection Method for the Genetic Engineering of Diapause Silkworm Egg Using Dimethyl Sulfoxide

Yamada

Mise

Yonemura

et al. 2023

JARQ

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“…S1A) (Doira et al, 1981). In Bombyx mori, ommochrome pigments also accumulate in the epidermis of the 1 st instar larva, and the larval ganglion (Osanai-Futahashi et al, 2012;Tomihara et…”

Section: B-t Mutations Reduce the Ommochrome-based Pigmentation In B ...mentioning

confidence: 99%

“…While only ommatin-type ommochrome pigments have been identified from compound eyes of Drosophila, B. mori has multiple types of ommochrome pigments which give their eggs and compound eyes dark coloration. Genes responsible for several mutants of ommochrome biosynthesis in B. mori have been identified (Quan et al, 2007;Komoto et al, 2009;Tatematsu et al, 2011;Osanai-Futahashi et al, 2016;Tomihara et al, 2021), and the red egg gene important for ommin-type ommochrome (Osanai-Futahashi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

A novel monocarboxylate transporter involved in 3-hydroxykynurenine transport for ommochrome coloration

Uchiyama

Takasu

Moriyama

et al. 2023

Preprint

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Ommochromes are widespread pigments in invertebrates utilized for screening pigments in compound eyes and for reddish coloration in epidermis and wings. Ommochromes are derived from 3-hydroxykynurenine (3OHK), which is incorporated into cells from hemolymph or synthesized from tryptophan within cells. While the synthetic pathway from tryptophan to 3OHK has been well characterized, the gene responsible for cellular uptake of 3OHK has been poorly understood. In the silkwormBombyx mori, adult compound eyes and eggs contain a mixture of ommochrome pigments. By using positional cloning method, we found that a novel monocarboxylate transporter, 3-hydroxykynurenine transporter (3OHKT), is responsible for the recessive mutantmaternal brown of Tsujita(b-t) ofB. mori. Inb-tmutant, the color of the eggs is light brown, whereas the color of the compound eyes is normal, and we identified a 2-kb deletion in3OHKTgene. TALEN-mediated knockout of3OHKTgene produced the same coloration phenotype asb-tmutant, and the complementation test betweenb-tmutant and3OHKTknockout strain proved that 3OHKT is responsible forb-tphenotype. 3OHKT protein was localized in the cellular membrane, and LC-MS analysis indicated that the uptake of 3OHK from hemolymph into the ovary was suppressed in theb-tmutant. Moreover, we confirmed that3OHKTgene is specifically expressed at the reddish region and the time of pigmentation in the pupal wing of nymphalid butterflies. RNA interference of 3OHKT prevented reddish pigmentation in wings, highlighting its general involvement in ommochrome-based pigmentation other than compound eyes.

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Reduced-representation libraries in insect genetics

Hopper

2023

Current Opinion in Insect Science

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Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of the brown egg 4 (b-4) mutant in the silkworm, Bombyx mori

Cited by 3 publications

References 68 publications

Development of an Injection Method for the Genetic Engineering of Diapause Silkworm Egg Using Dimethyl Sulfoxide

Development of an Injection Method for the Genetic Engineering of Diapause Silkworm Egg Using Dimethyl Sulfoxide

A novel monocarboxylate transporter involved in 3-hydroxykynurenine transport for ommochrome coloration

Reduced-representation libraries in insect genetics

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