Mutations in pheromone‐binding protein3 contribute to pheromone response variations in Plutella xylostella (L.) (Lepidoptera: Plutellidae)

Abstract

BACKGROUND: Diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most important crucifer pests. Commercial sex attractants have been developed to monitor and control P. xylostella. However, some studies have demonstrated a variety of pheromone responses of P. xylostella in different locations of the world. Soluble pheromone-binding proteins (PBPs), as a subfamily of odorant-binding proteins (OBPs), could selectively bind and transport pheromones across aqueous sensillar lymph to the surface of olfactory receptor neurons. It is worthy to study whether the mutation of PxylPBPs is one of the reasons for the different responses of sex attractors in different regions. RESULTS: In this study, P. xylostella males were collected from seven Chinese provinces, including Hainan, Guangdong, Yunnan, Fujian, Hunan, Zhejiang, and Hebei. PxylPBP1, PxylPBP2, and PxylPBP3 were cloned, and 3, 6, and 32 types of mutation pattern were identified, respectively. These mutation patterns were distributed in each province with different frequency. The results of fluorescence displacement binding assay and in silico simulation revealed that the three mutant PxylPBP3 were more sensitive to Z11-16:Ald than the reference protein (ACI28451). CONCLUSION: This result implied that mutation of PxylPBP3 may have contributed to regional differences in pheromone responses of P. xylostella.

Functional Disparity of Three Pheromone-Binding Proteins to Different Sex Pheromone Components in Hyphantria cunea (Drury)

“…polyphemus, L. dispar, and Plutella xylostella, ,, our results provide further support for the hypothesis that PBPs act as an additional layer of selectivity and participate in pheromone discrimination . The two aldehydes in the H.…”

“…Interestingly, HcunPBP3 exhibited a moderate binding affinity to both Z3, Z6-9S,10R-epoxy-21Hy and 1,Z3, Z6-9S,10R-epoxy-21Hy. Consistent with previous studies on A. polyphemus, L. dispar, and Plutella xylostella, 31,32,47 our results provide further support for the hypothesis that PBPs act as an additional layer of selectivity and participate in pheromone discrimination. 30 The two aldehydes in the H. cunea sex pheromone system are structurally somewhat similar to many type I pheromone components (i.e., C10−C18 unsaturated straight-chain acetates, aldehydes, or alcohols) and markedly different from most other type II sex pheromones, which lack a terminal functional group.…”

Structure dynamics reveal key residues essential for the sense of 1‐dodecanol by Cydia pomonella pheromone binding protein 2 (CpomPBP2)

“…Confirmation of Glu98 as a hot‐spot supports the assumption that residues forming H‐bond with ligands are usually crucial to the protein‐ligand interactions 24,32,51 . As a matter of experience, in protein‐ligand interactions involved with insect OBPs, key residues are usually those forming either H‐bond or hydrophobic interactions with ligand groups 26,32,44,53 . However, Arg109 providing a salt‐bridge interaction, a form of electrostatic interaction rarely described in protein–ligand interactions involved with insect OBPs, was determined as a second key residue for the CpomPBP2–Dod complex.…”

Transcriptome Analysis of Ophraella communa Male Reproductive Tract in Indirect Response to Elevated CO2 and Heat Wave

“…reproduction. Odorant binding proteins are well-known to facilitate the transfer of apolar odorants in the aqueous antennal perilymph during pheromone reception (Vogt et al, 1991;Shen et al, 2019). Among the DEGs identified for the TE vs. TEck comparison, two odorant binding proteins (Oc33781_g1 and Oc45230_g1) were identified.…”