Mutations in rrs , rpsL and gidB in streptomycin-resistant Mycobacterium tuberculosis isolates from Thailand

Smittipat, Nat; Juthayothin, Tada; Billamas, Pamaree; Jaitrong, Sarinya; Rukseree, Kamolchanok; Dokladda, Kanchana; Chaiyasirinroje, Boonchai; Disratthakit, Areeya; Chaiprasert, Angkana; Mahasirimongkol, Surakameth; Yanai, Hideki; Yamada, Norio; Tokunaga, Katsushi; Palittapongarnpim, Prasit

doi:10.1016/j.jgar.2015.11.009

Cited by 35 publications

(48 citation statements)

References 25 publications

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“…The combined detection of three rpsL and two gidB mutations provided 76.1% sensitivity and 96.4% specificity for identification of SMR in Thai M. tuberculosis isolates. Similar to another Thai study8, SMR was strongly associated to East Asian (Beijing lineage) (32/46 isolates, 69.6%) (OR, 2.7; 95% CI, 1.2–6.2; p=0.017) (Table 3). Moreover, rpsL mutations were predominant among SM resistant Beijing isolates (93.8%) (OR, 37.5; 95% CI, 5.9–236.6; p<0.001) while their presence was significantly lower among non-Beijing lineages (28.6%).…”

Section: Resultssupporting

confidence: 82%

“…Whereas, the 276A>C (Glu92Asp) gidB , a known marker for the Beijing isolates68, presented in 55/57 spoligotyped-Beijing (East Asian) and 2/34 EAI5 (Indo-Oceanic) isolates (Tables 1, 3). The discrepancies were further clarified by DTM-PCR, and it was found that the 55 Beijing and 2 EAI5 isolates with Glu92Asp carried RD105 deletion specific for Beijing lineage (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the low-level SM resistance (SMR) in M. tuberculosis is caused by mutations in a 7-methylguanosine (m 7 G) methyltransferase ( gidB ) gene which functions for methylation of G527 in 530-loop of 16S rRNA5. The frequencies and types of SMR associated mutations in the above-mentioned genes are dependent on the geographic areas and M. tuberculosis lineages678. In addition, some gidB SNPs are responsible as M. tuberculosis lineage signatures rather than SM resistant markers68.…”

Section: Introductionmentioning

confidence: 99%

“…The frequencies and types of SMR associated mutations in the above-mentioned genes are dependent on the geographic areas and M. tuberculosis lineages678. In addition, some gidB SNPs are responsible as M. tuberculosis lineage signatures rather than SM resistant markers68. Approximately 30% of SM resistant strains worldwide carried no mutation in those three genes and therefore, the contribution of alternative genes conferring intrinsic mechanism of SMR such as aminoglycosides efflux pump Tap ( Rv1258c ) and its transcriptional regulator, whiB7 ( Rv3197A ), have been suggested 491011.…”

Section: Introductionmentioning

confidence: 99%

“…However, to date, the information on M. tuberculosis mutations associated to SMR in Thailand is limited to only one study8. Therefore, we further expanded the investigation on the mutations of rpsL , rrs , gidB , and whiB7 in M. tuberculosis isolates collected from the different time and region of Thailand, and associated with their SMR and lineages.…”

Section: Introductionmentioning

confidence: 99%

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Mutations in Streptomycin Resistance Genes and Their Relationship to Streptomycin Resistance and Lineage ofMycobacterium tuberculosisThai Isolates

et al. 2017

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BackgroundStreptomycin (SM) is recommended by the World Health Organization (WHO) as a part of standard regimens for retreating multidrug-resistant tuberculosis (MDR-TB) cases. The incidence of MDR-TB in retreatment cases was 19% in Thailand. To date, information on SM resistance (SMR) gene mutations correlated to the SMR of Mycobacterium tuberculosis Thai isolates is limited. In this study, the mutations in rpsL, rrs, gidB, and whiB7 were investigated and their association to SMR and the lineage of M. tuberculosis were explored.MethodsThe lineages of 287 M. tuberculosis collected from 2007 to 2011 were identified by spoligotyping. Drug susceptibility profiles were evaluated by the absolute concentration method. Mutations in SMR genes of 46 SM-resistant and 55 SM-susceptible isolates were examined by DNA sequencing.ResultsThree rpsL (Lys43Arg, Lys88Arg, and Lys88Thr) and two gidB (Trp45Ter and Gly69Asp) mutations were present exclusively in the SM resistant M. tuberculosis. Lys43Arg rpsL was the most predominant SMR mutations (69.6%) and prevailed among Beijing isolates (p<0.001). No SMR-related mutation in was found rrs. The combination of rpsL and gidB mutations provided 76.1% sensitivity for detecting SMR in M. tuberculosis Thai isolates. whiB7 was not responsible for SMR in SM resistant isolates lacking rpsL and rrs mutations. The significance of the three gidB mutations, 276A>C, 615A>G, and 330G>T, as lineage signatures for Beijing and EAI were underscored. This study identified 423G>A gidB as a novel sub-lineage marker for EAI6-BGD1.ConclusionOur study suggested that the majority of SMR in M. tuberculosis Thai isolates were responsible by rpsL and gidB polymorphisms constantly providing the novel lineage specific makers.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mutations in Streptomycin Resistance Genes and Their Relationship to Streptomycin Resistance and Lineage ofMycobacterium tuberculosisThai Isolates

et al. 2017

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Resistance and tolerance of Mycobacterium tuberculosis to antimicrobial agents—How M. tuberculosis can escape antibiotics

Yuan

Duan

2022

WIREs Mechanisms of Disease

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Tuberculosis (TB) poses a serious threat to public health worldwide since it was discovered. Until now, TB has been one of the top 10 causes of death from a single infectious disease globally. The treatment of active TB cases majorly relies on various anti-tuberculosis drugs. However, under the selection pressure by drugs, the continuous evolution of Mycobacterium tuberculosis (Mtb) facilitates the emergence of drug-resistant strains, further resulting in the accumulation of tubercle bacilli with multiple drug resistance, especially deadly multidrug-resistant TB and extensively drug-resistant TB. Researches on the mechanism of drug action and drug resistance of Mtb provide a new scheme for clinical management of TB patients, and prevention of drug resistance. In this review, we summarized the molecular mechanisms of drug resistance of existing anti-TB drugs to better understand the evolution of drug resistance of Mtb, which will provide more effective strategies against drug-resistant TB, and accelerate the achievement of the EndTB Strategy by 2035.

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Evaluation of Mutations Related to Streptomycin Resistance in Mycobacterium tuberculosis Clinical Isolates

et al. 2022

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Mutations in rrs , rpsL and gidB in streptomycin-resistant Mycobacterium tuberculosis isolates from Thailand

Cited by 35 publications

References 25 publications

Mutations in Streptomycin Resistance Genes and Their Relationship to Streptomycin Resistance and Lineage ofMycobacterium tuberculosisThai Isolates

Mutations in Streptomycin Resistance Genes and Their Relationship to Streptomycin Resistance and Lineage ofMycobacterium tuberculosisThai Isolates

Resistance and tolerance of Mycobacterium tuberculosis to antimicrobial agents—How M. tuberculosis can escape antibiotics

Evaluation of Mutations Related to Streptomycin Resistance in Mycobacterium tuberculosis Clinical Isolates

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