Mutations inRAD3, MSH2, andRAD52 affect the rate of gene amplification in the yeastSaccharomyces cerevisiae

Peterson, Christopher; Kordich, Jennifer J.; Milligan, Laura; Bodor, Erika; Siner, Angela; Nagy, K.; Paquin, Charlotte E.

doi:10.1002/1098-2280(2000)36:4<325::aid-em8>3.0.co;2-b

Cited by 5 publications

(2 citation statements)

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“…In S. cerevisiae , estimated rates of duplications range from as low as 10 −10 amplifications/cell/division (Dorsey et al 1992; Peterson et al 2000) to 3.4 × 10 −6 amplifications/cell/division (Lynch et al 2008). However, the rate at which amplification and deletion alleles are spontaneously generated is highly variable and dependent on local genome architecture (Romero & Palacios 1997).…”

Section: The Role Of Copy Number Variation In Adaptation In Chemostatsmentioning

confidence: 99%

The functional basis of adaptive evolution in chemostats

2014

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Two central problems in biology are determining the molecular basis of adaptive evolution and understanding how cells regulate their growth. The chemostat is a device for culturing cells that provides great utility in tackling both of these problems: it enables precise control of the selective pressure under which organisms evolve and it facilitates experimental control of cell growth rate. The aim of this review is to synthesize results from studies of the functional basis of adaptive evolution in long-term chemostat selections using Escherichia coli and Saccharomyces cerevisiae. We describe the principle of the chemostat, provide a summary of studies of experimental evolution in chemostats and use these studies to assess our current understanding of selection in the chemostat. Functional studies of adaptive evolution in chemostats provide a unique means of interrogating the genetic networks that control cell growth, which complements functional genomic approaches and quantitative trait loci (QTL) mapping in natural populations. An integrated approach to the study of adaptive evolution that accounts for both molecular function and evolutionary processes is critical to advancing our understanding of evolution. By renewing efforts to integrate these two research programs experimental evolution in chemostats is ideally suited to extending this functional synthesis to the study of genetic networks.

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Section: The Role Of Copy Number Variation In Adaptation In Chemostatsmentioning

confidence: 99%

The functional basis of adaptive evolution in chemostats

2014

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“…1). Amplification strains A4C-EA1 and A4C-EA2 were isolated by Dorsey et al [1993] and strains A4C-EA3 and A4C-EA4 were isolated in a separate experiment [Peterson et al, 2000]. We chose to analyze these four independent amplifications together because of the similar location and appearance of the amplified DNA in Southern blots of pulsed-field gels hybridized to the ADH4 gene ( Fig.…”

Section: Circular Amplifications Of the Adh4:cup1 Cassettementioning

confidence: 99%

Formation of circular amplifications inSaccharomyces cerevisiae by a breakage-fusion-bridge mechanism

Moore

Martin

Dorsey

et al. 2000

Environ. Mol. Mutagen.

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Components of nucleotide excision repair and DNA damage tolerance in Arabidopsis thaliana

Kunz

Anderson

Osmond

et al. 2005

Environ and Mol Mutagen

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As obligate phototrophs, and despite shielding strategies, plants sustain DNA damage caused by UV radiation in sunlight. By inhibiting DNA replication and transcription, such damage may contribute to the detrimental effects of UV radiation on the growth, productivity, and genetic stability of higher plants. However, there is evidence that plants can reverse UV-induced DNA damage by photoreactivation or remove it via nucleotide excision repair. In addition, plants may have mechanisms for tolerating UV photoproducts as a means of avoiding replicative arrest. Recently, phenotypic characterization of plant mutants, functional complementation studies, and cDNA analysis have implicated genes isolated from the model plant Arabidopsis thaliana in nucleotide excision repair or tolerance of UV-induced DNA damage. Here, we briefly review features of these processes in human cells, collate information on Arabidopsis homologs of the relevant genes, and summarize the experimental findings that link certain of these plant genes to nucleotide excision repair or damage tolerance.

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Mutations inRAD3, MSH2, andRAD52 affect the rate of gene amplification in the yeastSaccharomyces cerevisiae

Cited by 5 publications

References 54 publications

The functional basis of adaptive evolution in chemostats

The functional basis of adaptive evolution in chemostats

Formation of circular amplifications inSaccharomyces cerevisiae by a breakage-fusion-bridge mechanism

Components of nucleotide excision repair and DNA damage tolerance in Arabidopsis thaliana

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