Mutations of the p53 gene in the stool of patients with resectable colorectal cancer

Detection ofAPCMutations in Fecal DNA from Patients with Colorectal Tumors

“…Several groups have reported that mutations in cancer-related genes can be detected in the stool of patients with colorectal cancer. [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] However, the sensitivities and specificities of these approaches have been limited by technical impediments or the low frequencies of detectable mutations in any specific gene.…”

Fortnightly review: screening for asymptomatic colorectal cancer

“…The range of somatic mutations that can be detected in faecal DNA64 65 66 is too limited to allow practical genetic screening studies at present, and many molecular biological assays are unsuitable for routine clinical use. However, if developments in molecular biology continue to unfold at their current pace the concept of a non-invasive, sensitive, and specific genetic screening test for colorectal cancer may not be as far fetched as it seems.…”

Molecular stool screening for colorectal cancer

“…Mutation studies on tumour samples are carried out to determine the accuracy of mutation detected from stool. Samples are either taken fresh from operation and stored at ultralow temperatures85, 88 or kept in paraffin‐embedded tissue blocks until processing86. To increase sensitivity the yield of mutant DNA from these tumour samples can be increased by microdissection, a technique in which tumour tissue is identified and separated from normal tissue residing in the resected specimen89, 90.…”