Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis

Ray, Celeste; Hay, Regine E.; rd, H L Carter; Moran, Charles P.

doi:10.1128/jb.163.2.610-614.1985

Cited by 74 publications

(16 citation statements)

References 14 publications

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“…Comparison of catB promoter with σ B ‐dependent promoters of B. subtilis ( sigB , Kalman et al ., 1990; ctc , Moran et al ., 1981; gtaB , Varon et al ., 1993; trxA , Scharf et al ., 1998; katE , Engelmann et al ., 1995) and endoH promoter of S. plicatus (Westpheling et al ., 1985). Underlined nucleotides in ctc promoter are those shown to be important for promoter activity in vitro (Tatti and Moran, 1984) and in vivo (Ray et al ., 1985).…”

Section: Resultsmentioning

confidence: 99%

A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor

Cho

Lee

Roe

2000

Molecular Microbiology

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SummaryStreptomyces coelicolor produces at least three catalases, the expression of which varies under different conditions. We characterized a gene (catB ) for developmentally controlled catalase of 779 amino acids (83408 Da), homologous to KatE of Escherichia coli and Bacillus subtilis. Expression of the catB gene increased at the stationary phase in liquid culture and after the onset of differentiation on solid culture. It was also increased by osmotic treatments. Transcription was initiated from a promoter (catBp), whose sequence (ATGCCTCG-N 13 -GGGTAC) resembled promoters recognized by s B of B. subtilis. CatB protein underwent proteolytic cleavage of its N-terminal 95 amino acids and was secreted to the medium when cells sporulated. Disruption of the catB gene caused impairment in the formation of aerial mycelium and reduction in the synthesis of undecylprodigiosin. On the contrary, hyperproduction of actinorhodin was observed in accordance with the increase in act II-ORF4 transcription. In addition, catB mutant became hypersensitive to osmotic stresses. These results suggest that regulated synthesis of CatB protein is necessary to ensure proper differentiation as well as to protect S. coelicolor cells against osmotic stresses.

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Section: Resultsmentioning

confidence: 99%

A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor

Cho

Lee

Roe

2000

Molecular Microbiology

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“…The oB-dependent +1B signal maps to the thymidine at nucleotide (nt) 1185 and is preceded by the sequence 5'-ATGlTTAT-13 bp-GGGTAA-3' (nt 11 51 -1 177). This sequence and spacing are very similar to elements found in other oB-dependent promoters (Antelmann et a/., 1995;Boylan etal., 1991 ;Engelmann etal., 1995;Kalman et a/., 1990;Maul et a/., 1995;Moran eta/., 1982;Varon et a/., 1993), and the match is exact at the five positions known to be important for activity of the well-characterized oBdependent ctc promoter (Ray et a/., 1985;Tatti and Moran, 1984; see Fig. 3).…”

Section: ; S T T T T T a T A A M T T ' T T 1 ' T G T A T T T C~g T A mentioning

confidence: 91%

Bacillus subtilis operon under the dual control of the general stress transcription factor σ^B and the sporulation transcription factor σ^H

Varón

Brody

Price

1996

Molecular Microbiology

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The sigma B transcription factor of Bacillus subtilis is activated in response to a variety of environmental stresses, including those imposed by entry into the stationary-growth phase, and by heat, salt or ethanol challenge to logarithmically growing cells. Although sigma B is thought to control a general stress regulon, the range of cellular functions it directs remains largely unknown. Our approach to understand the physiological role of sigma B is to characterize genes that require this factor for all or part of their expression, i.e. the csb genes. In this study, we report that the transposon insertion csb40::Tn917lac identifies an operon with three open reading frames, the second of which resembles plant proteins induced by desiccation stress. Primer-extension and operon-fusion experiments showed that the csb40 operon has a sigma B-dependent promoter which is strongly induced by the addition of salt to logarithmically growing cells. The csb40 operon also has a second, sigma H-dependent promoter that is unaffected by salt addition. These results provide support for the hypothesis that sigma B controls a general stress regulon, and indicate that the sigma B and sigma H regulons partly overlap. We suggest that in addition to its acknowledged role in the sporulation process, sigma H is also involved in controlling a subclass of genes that are broadly involved in a general stress response.

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“…Northern blotting and primer extension analysis were performed using standard methods (Sambrook & Russell, 2001). An established method was used for spectrophotometric measurement of XylE (catechol 2,3-dioxygenase) activity (Ray et al, 1985). Protein concentrations were determined using the BCA protein assay method according to the instructions of the manufacturer (Pierce Biotechnology Inc.).…”

Section: Other Methodsmentioning

confidence: 99%

The master transcription factor Spo0A is required for poly(3-hydroxybutyrate) (PHB) accumulation and expression of genes involved in PHB biosynthesis in Bacillus thuringiensis

Chen¹,

Tsai²,

Pan³

et al. 2010

FEMS Microbiology Letters

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Bacillus thuringiensis is a gram-positive spore-forming bacterium that can accumulate poly(3-hydroxybutyrate) (PHB) as a carbon and energy storage substance in response to nutritional stress. The regulatory mechanism for PHB biosynthesis in B. thuringiensis and diverse Bacillus species is still poorly understood. We now report that disruption of the sigH gene or the gene encoding the master sporulation transcription factor Spo0A severely impaired PHB accumulation in B. thuringiensis. Complementation of the spo0A mutation with the spo0A gene restored PHB accumulation. We have found that the requirement of Spo0A for PHB accumulation is independent of the transition state regulator AbrB and of loss of sporulation ability. We also show that Spo0A is required for the expression of three genes involved in PHB biosynthesis. These findings have uncovered a new role of Spo0A in the regulation of stationary-phase-associated cellular events.

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Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis

Cited by 74 publications

References 14 publications

A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor

A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor

Bacillus subtilis operon under the dual control of the general stress transcription factor σ^B and the sporulation transcription factor σ^H

The master transcription factor Spo0A is required for poly(3-hydroxybutyrate) (PHB) accumulation and expression of genes involved in PHB biosynthesis in Bacillus thuringiensis

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Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis

Cited by 74 publications

References 14 publications

A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor

A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor

Bacillus subtilis operon under the dual control of the general stress transcription factor σB and the sporulation transcription factor σH

The master transcription factor Spo0A is required for poly(3-hydroxybutyrate) (PHB) accumulation and expression of genes involved in PHB biosynthesis in Bacillus thuringiensis

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Bacillus subtilis operon under the dual control of the general stress transcription factor σ^B and the sporulation transcription factor σ^H