Mutations

Sébald, M

doi:10.1007/978-1-4615-7087-5_5

Cited by 4 publications

(3 citation statements)

References 112 publications

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“…There are indications that familiar systems from aerobes, such as recA, may be missing, or at least significantly different, in anaerobes (Reed, 1988;Sebald, 1993). There are indications that familiar systems from aerobes, such as recA, may be missing, or at least significantly different, in anaerobes (Reed, 1988;Sebald, 1993).…”

Section: Mutagenesis In Rumen Bacteriamentioning

confidence: 99%

The Rumen Microbial Ecosystem

Hobson¹,

Stewart²

1997

352

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Section: Mutagenesis In Rumen Bacteriamentioning

confidence: 99%

The Rumen Microbial Ecosystem

Hobson¹,

Stewart²

1997

352

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“…For mutagenesis, 10 ml of exponentialphase cells of C. butyricum DSM 5431 was treated in Hungate tubes with 0.1 ml of 1% (wt/vol) N-methyl-NЈ-nitro-N-nitrosoguanidine (NTG) at 34ЊC for 60 min. NTG is the mutagen most commonly used to obtain clostridial mutants (24), and under the conditions we used, about 1 of 100 cells survived. Following centrifugation and two washings with fresh sterile RCM, cell samples were inoculated into RCM supplemented with 108 mM glycerol (10 g/liter).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Clostridium butyricum DSM 5431 Mutants with Increased Resistance to 1,3-Propanediol and Altered Production of Acids

Abbad-Andaloussi¹,

Manginot-Dürr²,

Amine³

et al. 1995

Appl Environ Microbiol

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Clostridium butyricum mutants were isolated from the parent strain DSM 5431 after mutagenesis with N-methyl-N-nitro-N-nitrosoguanidine and two selection procedures: osmotic pressure and the proton suicide method. Isolated mutants were more resistant to glycerol and to 1,3-propanediol (1,3-PD) than was the wild type, and they produced more biomass. In batch culture on 62 g of glycerol per liter, the wild type produced more acetic acid than butyrate, with an acetate/butyrate ratio of 5.0, whereas the mutants produced almost the same quantities of both acids or more butyrate than acetate with acetate/butyrate ratios from 0.6 to 1.1. The total acid formation was higher in the wild-type strain. Results of analysis of key metabolic enzymatic activities were in accordance with the pattern of fermentation product formation: either the butyrate kinase activity increased or the acetate kinase activity decreased in cell extracts of the mutants. A decreased level of the hydrogenase and NADH-ferredoxin activities concomitant with an increase in ferredoxin-NAD ؉ reductase activities supports the conclusion that the maximum percentage of NADH available and used for the formation of 1,3-PD was higher for the mutants (97 to 100%) than for the wild type (70%). In fed-batch culture, at the end of the fermentation (72 h for the wild-type strain and 80 to 85 h for the mutants), 44% more glycerol was consumed and 50% more 1,3-PD was produced by the mutants than by the wild-type strain.

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“…For mutagenesis, 9 ml of exponential-phase-cell culture of C. butyricum E5 was treated in Hungate tubes with 1 ml of 0.1% (wt/vol) N-methyl-NЈnitro-N-nitrosoguanidine (NTG) at 34ЊC for 60 min. NTG is the mutagen most commonly used to obtain clostridial mutants (10), and, under the conditions that we used, approximately 1 of 100 cells survived. After centrifugation and two washings with fresh sterile reinforced clostridial medium (RCM) (Oxoid Ltd., Basingstoke, England), cell samples were inoculated into RCM supplemented with 108 mM glycerol (10 g/liter) and were grown overnight at 34ЊC.…”

mentioning

confidence: 99%

Properties of Allyl Alcohol-Resistant Mutants of Clostridium butyricum Grown on Glycerol

Abbad-Andaloussi¹,

Amine²,

Gérard³

et al. 1996

Appl Environ Microbiol

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Mutants of Clostridium butyricum E5 exhibiting resistance to allyl alcohol which produced the same quantities of 1,3-propanediol as the wild-type strain but more acetate than butyrate were isolated. The acetatebutyrate formation plays a major function in the regulation of the internal redox balance. Allyl alcohol resistance can be attributed not to the loss of 1,3-propanediol dehydrogenase but to a shift in the reductive properties of the enzyme. The data support the view that cellular regulation is modified to avoid intracellular accumulation of 3-hydroxypropionaldehyde.

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Mutations

Cited by 4 publications

References 112 publications

The Rumen Microbial Ecosystem

The Rumen Microbial Ecosystem

Isolation and Characterization of Clostridium butyricum DSM 5431 Mutants with Increased Resistance to 1,3-Propanediol and Altered Production of Acids

Properties of Allyl Alcohol-Resistant Mutants of Clostridium butyricum Grown on Glycerol

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