Mutual Interactions of Boar Seminal Plasma Proteins Studied by Immunological and Chromatographic Methods

Abstract

Mutual specific interactions between protein components of boar seminal plasma were shown. These interactions participate in the formation of aggregated forms of proteins in seminal plasma and probably also in the arrangement and remodelling of protein coating layers of sperm. Aggregation of seminal plasma proteins is probably an important phenomenon in the fertilization process.

Seminal Plasma Proteins as Modulators of the Sperm Function and Their Application in Sperm Biotechnologies

“…2005). Later, during ejaculation, aggregated and monomeric forms of SP proteins secreted by the accessory sexual glands will further remodel sperm membrane (Manásková et al. 2003).…”

“…Firstly, morphologically complete spermatozoa leave the testis to enter into the epididymis where they will suffer a series of changes as a consequence of their interaction with the epididymal environment (Myles et al 1987;Lum and Blobel 1997;Dacheux et al 2005). Later, during ejaculation, aggregated and monomeric forms of SP proteins secreted by the accessory sexual glands will further remodel sperm membrane (Mana´skova´et al 2003). Because sperm cells have a limited biosynthetic ability (Amann et al 1993), the interaction with the different SP proteins found along their journey to the fertilization site will have a profound impact on sperm functionality.…”

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

“…Specific proteins from the spermadhesin family, such as porcine seminal protein (PSP), AWN, and AQN, coat the sperm surface during ejaculation, producing structural changes to the sperm plasma membrane that affect sperm performance during the fertilization process (Maná sková et al, 2003). The major seminal plasma glycoprotein (PSP-I), isolated and identified from boar seminal plasma by Rutherfurd et al (1992), may prevent premature capacitation and the acrosome reaction (Kwok et al, 1993;Tö pfer-Petersen et al, 1998).…”

Comparative Studies on the Aggregation Behavior of HBPs from Human Seminal Plasma by Dynamic Light Scattering

“…SPPs are known to interact with various types of ligands, such as saccharide moiety of glycoproteins, polysaccharides of glycosaminoglycan, phospholipids, lipoproteins, collagen, protease inhibitors, sperm surface, and zona pellucida [12,[21][22][23][24]. Specific interactions between SPPs lead to the formation of higher-molecular-mass aggregates.…”