Mx expression in gilthead sea bream (Sparus aurata L.) in response to poly I:C, bacterial LPS and chromosomal DNA: Preliminary study

Bravo, Jimena; Acosta, F.; Padilla, Daniel; Real, Fernando

doi:10.1016/j.fsi.2011.04.016

Cited by 13 publications

(5 citation statements)

References 22 publications

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“…As in other studies carried out in vivo for the stimulation of Mx using Poly I: C [67], the expression of this gene showed its highest values during the first days, later decreasing to baseline values. Specifically, in this study in seabream, the highest peak of expression was obtained at 12 h post-stimulation with Poly I: C, regardless of the diet used, recovering the basal expression levels at 48 h, in agreement with previous studies in this species [26]. On the contrary, in Atlantic salmon, the up-regulation of Mx gene by induction with Poly I: C remains for up to 9 days [67].…”

Section: Effect Of Dietary Se In Gene Expression Of MX Gene and Immune Related Genessupporting

confidence: 92%

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Organic Selenium (OH-MetSe) Effect on Whole Body Fatty Acids and Mx Gene Expression against Viral Infection in Gilthead Seabream (Sparus aurata) Juveniles

Tseng

Domı́nguez

Bravo

et al. 2021

Animals

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The supplementation of fish diets with OH-SeMet reduces oxidative stress and modulates immune response against bacterial infection. However, despite the importance of essential polyunsaturated fatty acids in fish nutrition and their high risk of oxidation, the potential protective effect of OH-SeMet on these essential fatty acids has not been studied in detail. Moreover, while viral infection is very relevant in seabream production, no studies have focused the Se effects against viral infection. The aim of the present study was to assess the impact of dietary supplementation with OH-SeMet on gilthead seabream fatty acid profiles, growth performance and response against viral infection. Gilthead seabream juveniles (21.73 ± 0.27 g) were fed for 91 days with three experimental diets, a control diet without supplementation of Se (0.29 mg Se kg diet−1) and two diets supplemented with OH-SeMet (0.52 and 0.79 mg Se kg diet−1). A crowding stress test was performed at week 7 and an anti-viral response challenge were conducted at the end of the feeding trial. Selenium, proximate and fatty acid composition of diets and body tissues were analyzed. Although fish growth was not affected, elevation in dietary Se proportionally raised Se content in body tissues, increased lipid content in the whole body and promoted retention and synthesis of n-3 polyunsaturated fatty acids. Specifically, a net production of DHA was observed in those fish fed diets with a higher Se content. Additionally, both monounsaturated and saturated fatty acids were significantly reduced by the increase in dietary Se. Despite the elevation of dietary Se to 0.79 mg kg−1 not affecting basal cortisol levels, 2 h post-stress plasma cortisol levels were markedly increased. Finally, at 24 h post-stimulation, dietary OH-SeMet supplementation significantly increased the expression of the antiviral response myxovirus protein gene, showing, for the first time in gilthead seabream, the importance of dietary Se levels on antiviral defense.

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Section: Effect Of Dietary Se In Gene Expression Of MX Gene and Immune Related Genessupporting

confidence: 92%

“…Mx proteins belong to the superfamily of GTPases involved in intracellular membrane remodeling and intracellular trafficking. They interfere with viral replication in different states by inhibiting viral polymerase in the nucleus and by binding viral components in the cytoplasm [26].…”

Section: Introductionmentioning

confidence: 99%

Organic Selenium (OH-MetSe) Effect on Whole Body Fatty Acids and Mx Gene Expression against Viral Infection in Gilthead Seabream (Sparus aurata) Juveniles

Tseng

Domı́nguez

Bravo

et al. 2021

Animals

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“…Finally, Mx proteins are the molecular effector of interferon, one of the most known mechanisms against virus [54]. Mx gene can be stimulated by bacteria in fish, as different authors have shown [55,56]. No statistically significant differences were found with respect to the control, showing a very slight expression of the Mx gene in the spleen, while in the liver and kidney, subexpression of this gene was observed.…”

Section: Discussionmentioning

confidence: 98%

Screening of New Potential Probiotics Strains against Photobacterium damselae Subsp. piscicida for Marine Aquaculture

Falcón

Padilla

Real

et al. 2021

Animals

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On intensive fish farms, 10% of the population dies exclusively from pathogens, and Photobacterium damselae subsp. Piscicida (Ph. damselae subsp. Piscicida), the bacteria causing pasteurellosis in marine aquaculture, is one of the major pathogens involved. The objective of this study was to obtain new probiotic strains against pasteurellosis in order to limit the use of chemotherapy, avoiding the environmental repercussions generated by the abusive use of these products. In this study, 122 strains were isolated from the gills and intestines of different marine fish species and were later evaluated in vitro to demonstrate the production of antagonistic effects, the production of antibacterial substances, adhesion and growth to mucus, resistance to bile and resistance to pH gradients, as well as its harmlessness and the dynamic of expression of immune-related genes by real-time PCR after administration of the potential probiotic in the fish diet. Only 1/122 strains showed excellent results to be considered as a potential probiotic strain and continue its characterization against Ph. damselae subsp. piscicida to determine its protective effect and elucidating in future studies its use as a possible probiotic strain for marine aquaculture.

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“…Specific PCR primers for Mx, were designed using the GeneRunner ® software. For the amplification of the genes IL-1b, TNF-a and Cox-2 we used the sequence of primers described by [21]; the sequence described by Roca et al (2008) [22] for IL-Ir2 gene, and the sequence described by Bravo et al (2011) [9] for b-actin. b-actin was use as housekeeping gene.…”

Section: Analysis Of Gene Expressionmentioning

confidence: 99%

“…In human and other mammals, IFNs are the first line of defence against virus infections, Mx is a protein induced by IFN that interfere with the virus replication by inhibiting viral polymerase [9] Mx gene has been cloned in some fish species such as rainbow trout [10] Atlantic salmon [11] Atlantic halibut [12] Japanese flounder [13] puffer fish [14], sea bream [15] and Senegalese sole [16] Mx gene expression in fish has been demonstrated after dsRNA virus infection, LPS inoculation and some bacterin vaccination. In this study we wanted to analyze if our bacterin was able to enhance TNF-a, IL-1b, IL-1r2, COX-2 and Mx gene expression in vaccinated fish.…”

Section: Introductionmentioning

confidence: 99%

Immunization of sea bream (Sparus aurata) juveniles against Photobacterium damselae subsp. piscicida by short bath: Effect on some pro-inflammatory molecules and the Mx gene expression

Padilla

Bravo

Román

et al. 2015

Fish & Shellfish Immunology

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Mx expression in gilthead sea bream (Sparus aurata L.) in response to poly I:C, bacterial LPS and chromosomal DNA: Preliminary study

Cited by 13 publications

References 22 publications

Organic Selenium (OH-MetSe) Effect on Whole Body Fatty Acids and Mx Gene Expression against Viral Infection in Gilthead Seabream (Sparus aurata) Juveniles

Organic Selenium (OH-MetSe) Effect on Whole Body Fatty Acids and Mx Gene Expression against Viral Infection in Gilthead Seabream (Sparus aurata) Juveniles

Screening of New Potential Probiotics Strains against Photobacterium damselae Subsp. piscicida for Marine Aquaculture

Immunization of sea bream (Sparus aurata) juveniles against Photobacterium damselae subsp. piscicida by short bath: Effect on some pro-inflammatory molecules and the Mx gene expression

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