MXene-based cytosensor for the detection of HER2-positive cancer cells using CoFe2O4@Ag magnetic nanohybrids conjugated to the HB5 aptamer

Vajhadin, Fereshteh; Mazloum-Ardakani, Mohammad; Shahidi, Maryamsadat; Moshtaghioun, Seyed Mohammad; Haghiralsadat, Fateme; Ebadi, Azar; Amini, Abbas

doi:10.1016/j.bios.2021.113626

Cited by 62 publications

(28 citation statements)

References 50 publications

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“…The exosomes used in the experimental verification stage were extracted from cultured tumor cells by traditional ultracentrifugation. Different concentrations of exosomes were added to the capture probe solution, and the double rings were opened by the high affinity between the aptamer and HER2 protein over-expressed on the membrane of the SK-BR3-derived exosomes ( Liu et al, 2019 ; Vajhadin et al, 2022 ). The terminal G4 sequence was exposed while tightly capture exosomes.…”

Section: Resultsmentioning

confidence: 99%

A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

Chen

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). The whole reaction process can be completed by several simple steps, which realizes washing-free and labor-saving. With simplified operation procedures and high repeatability, the linear detection range for this developed fluorescent biosensing strategy to breast cancer cell-derived exosomes is from 2.5 × 105 to 1.00 × 107 particles/ml, and the limit of detection is down to 0.54 × 105 particles/ml.

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Section: Resultsmentioning

confidence: 99%

A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

Chen

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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“…For Fe2p high-resolution XPS spectrum (Fig. 2C), four peaks at 712.11 (Fe2p3/2), 714.23 (Fe2p1/2), 720.14 (Fe2p1/2) and 726.36 eV (Fe2p3/2) were observed [37]. The peaks at 712.16, and 726.33 eV were attributed to Fe 3þ ions in the octahedral, and Fe 3þ ions in tetrahedral sites, respectively [47].…”

Section: Characterizations Of Cofe 2 O 4 @Ag Nanocompositementioning

confidence: 98%

“…The color shifted from dark brown to light brown after 20 min, indicating the CoFe 2 O 4 @Ag nanocomposite. This nanocomposite was centrifuged at 1000 rpm for 20 min and collected with a magnet, then dried at 50 C [36,37].…”

Section: Synthesis Of Cofe 2 O 4 and Cofe 2 O 4 @Ag Nanocomposite As ...mentioning

confidence: 99%

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Electrochemical neuron-specific enolase (NSE) immunosensor based on CoFe2O4@Ag nanocomposite and AuNPs@MoS2/rGO

Karaman

Bölükbaşı

Yola

et al. 2022

Analytica Chimica Acta

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“…Additionally, it provides decent sensitivity and selectivity against HER2-positive cells detection in blood samples. Therefore, this CoFe 2 O 4 @Ag magnetic nanohybrids and MXenes-based aptacytosensor hold promise to screen cancer progression cost-effectively [ 220 ]. The MXene-based 2D material when used in combination with aptamer as biorecognition element for the fabrication of electrochemical aptasensor has enhanced the sensitivity of detection of cancer-associated biomarkers many folds with a linear range of detection as revealed by several studies discussed in this section.…”

Section: Mxene-enabled Advanced Electrochemical Aptasensors For Cance...mentioning

confidence: 99%

Next-Generation Intelligent MXene-Based Electrochemical Aptasensors for Point-of-Care Cancer Diagnostics

et al. 2022

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Delayed diagnosis of cancer using conventional diagnostic modalities needs to be addressed to reduce the mortality rate of cancer. Recently, 2D nanomaterial-enabled advanced biosensors have shown potential towards the early diagnosis of cancer. The high surface area, surface functional groups availability, and excellent electrical conductivity of MXene make it the 2D material of choice for the fabrication of advanced electrochemical biosensors for disease diagnostics. MXene-enabled electrochemical aptasensors have shown great promise for the detection of cancer biomarkers with a femtomolar limit of detection. Additionally, the stability, ease of synthesis, good reproducibility, and high specificity offered by MXene-enabled aptasensors hold promise to be the mainstream diagnostic approach. In this review, the design and fabrication of MXene-based electrochemical aptasensors for the detection of cancer biomarkers have been discussed. Besides, various synthetic processes and useful properties of MXenes which can be tuned and optimized easily and efficiently to fabricate sensitive biosensors have been elucidated. Further, futuristic sensing applications along with challenges will be deliberated herein.

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MXene-based cytosensor for the detection of HER2-positive cancer cells using CoFe2O4@Ag magnetic nanohybrids conjugated to the HB5 aptamer

Cited by 62 publications

References 50 publications

A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

Electrochemical neuron-specific enolase (NSE) immunosensor based on CoFe2O4@Ag nanocomposite and AuNPs@MoS2/rGO

Next-Generation Intelligent MXene-Based Electrochemical Aptasensors for Point-of-Care Cancer Diagnostics

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