MXene Enhanced Photoactivity of Bi₂O₃/Bi₂S₃ Heterojunction with G-wire Superstructure for Photoelectrochemical Detection of TET1 Protein

Abstract

Ten-eleven translocation 1 (TET1) protein has the potential to accelerate the oxygenation of 5-methylcytosine to 5hydroxymethylcytosine (5hmC); then the −CH 2 OH of 5hmC can further covalently react with −SH catalyzed by M.HhaI methyltransferase. A brand-new photoelectrochemical (PEC) detection technique for the TET1 protein was created in light of this. For this objective, the Bi 2 O 3 /Bi 2 S 3 heterojunction was first prepared by a one-pot hydrothermal method and served for photosensitive materials. For further enhancing the photoactivity, Bi 2 O 3 /Bi 2 S 3 was blended with MXene to form an energy bandmatched structure, thus improving the migration kinetics of photogenerated carriers. For achieving a high sensitivity of detection, a DNA Walker incorporated with the nicking endonuclease (Nb.BbvCI enzyme)-assisted signal amplification strategy was presented to output exponential G-quadruplex fragments. Self-assembly of the free G-quadruplex sequence into a G-wire superstructure with the assistance of Mg 2+ provided more loading sites for MB and amplified the PEC signal. The linear range of the biosensor was 0.1−10 μg/mL with a detection limit of 0.024 μg/mL (S/N = 3) for TET1 protein under optimal experimental conditions. The suitability of the proposed method was evaluated by inhibitor screening experiments and the influence of environmental degradation on the activity of TET1 protein.

Tetrahedral DNA Nanostructure-Engineered Paper-Based Electrochemical Aptasensor for Fumonisin B1 Detection Coupled with Au@Pt Nanocrystals as an Amplification Label

“…As shown in Figure E, X-ray diffraction (XRD) analysis was used to characterize the crystallographic structure of MXenes and AuNPs@MXenes. With regard to MXenes, it displayed multiple characteristic diffraction peaks and was consistent with the previously reported XRD results . Moreover, five additional diffraction peaks at 38.2°, 44.5°, 64.7°, 77.5°, and 81.8° in the AuNPs@MXenes composite can be attributed to the (111), (200), (220), (311), and (222) planes of face-centered cubic Au [Joint Committee on Powder Diffraction Standards (JCPDS) number 04-0784], respectively .…”

“…With regard to MXenes, it displayed multiple characteristic diffraction peaks and was consistent with the previously reported XRD results. 25 Moreover, five additional diffraction peaks at 38.2°, 44.5°, 64.7°, 77.5°, and 81.8°in the AuNPs@MXenes composite can be attributed to the (111), ( 200), ( 220), (311), and (222) planes of facecentered cubic Au [Joint Committee on Powder Diffraction Standards (JCPDS) number 04-0784], respectively. 26 The Xray photoelectron spectroscopy (XPS) survey spectra of AuNPs@MXenes (Figure S3 of the Supporting Information) and details are provided in the Supporting Information.…”

MXene-Enhanced Bi2S3/CdIn2S4 Heterojunction Photosensitive Gate for DEHP Detection in a Signal-On OPECT Aptamer Biosensor

“…33,34 MXene has been reported to have excellent visible light absorption and high conductivity, which facilitate the separation and transfer of photogenerated carriers, thereby improving the optoelectronic properties of semiconductor materials. 35,36 In summary, a ternary system was constructed to greatly enhance photoelectric activity.…”

“…To improve its OPECT response, an effective approach involves constructing energy-band-matched architectures by incorporating other nanomaterials capable of full-spectrum absorbance. MXene is a novel two-dimensional (2D) material that is rapidly gaining momentum in environmental, chemical, and medical applications due to its high surface area, excellent chemical stability, thermal conductivity, and environmental compatibility. , MXene has been reported to have excellent visible light absorption and high conductivity, which facilitate the separation and transfer of photogenerated carriers, thereby improving the optoelectronic properties of semiconductor materials. , In summary, a ternary system was constructed to greatly enhance photoelectric activity.…”

Efficient sensitization of CdTe QDs towards PTCDA for sensitive photoelectrochemical Hg2+ assay

“…To date, PEC biosensors have not only gained increasing attention but have also played a critical role in molecular assays, environmental analyses, food safety, and medical research, promoting sensitive analysis of various targets such as proteins, nucleic acids, small molecules, and metals. [8][9][10] Consequently, there is a great development potential to construct PEC biosensors for detecting Hg 2+ .…”