My First Biolab

Gome, Gilad; Fein, Yuval; Waksberg, Julian; Maayan, Yuval; Grishko, Andrey; Wald, Iddo Yehoshua; Zuckerman, Oren

doi:10.1145/3290607.3313081

Cited by 31 publications

(3 citation statements)

References 9 publications

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“…Supervision over cultivation in real-time can provide insights into scaled-out MSUBs in the bioprocess. For example, an MSUB that has low seeding efficiency can be monitored in the first days of cultivation using microscopy or with a reagent such as Alamar Blue and be replaced or reseeded, an MSUB that got contaminated can be sensed by sampling the media for turbidity manually or through the film with a light sensor [57] discarded, potentially saving time and resources for a bioproduction facility that operates in a scale-out model. This supervision can be achieved by adding real time sensors to each MSUB or by a monitoring station.…”

Section: Discussionmentioning

confidence: 99%

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Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat

Gome,

Chak,

Tawil

et al. 2024

Preprint

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One of the main challenges of cultured meat is reducing the costs of production, often described as scaling. The main components of the cultivation process include: cells, media, scaffolds and bioreactors. This work presents an alternative to current stainless steel or glass bioreactors and uses food-grade plant-based scaffolds. Bioreactors must be sterile and leak-free to maintain a proper environment for cell growth. Thermoplastic films are a popular material for constructing these fluidic systems, but traditional methods of welding these film are expensive and do not allow rapid prototyping resulting in high research and development costs for companies. In this study, a laser welding method was developed to join thermoplastic films in a way that prevents contamination and leaks, affording effective macro-fluidics fabrication. This technique was tested using polyethylene (PET) films and a laser cutter operating with settings calibrated for the material to be welded or cut. The laser welding method was found to produce strong, leak-free seals in PET films with minimal heat-induced damage to the films. The ability to design fluidics, chambers and ports of various size. Using this method afforded the incorporation of plant-based scaffolds from food-grade plants (Rice). The laser welding method developed in this study provides a reliable, contamination-free method for the rapid fabrication of fluidic systems for cell cultivation. This technique has the potential to be compatible with a vast range of cell types and scaffolds and to be widely adopted in tissue engineering for regenerative medicine and food applications such as cultured meat.

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Section: Discussionmentioning

confidence: 99%

“…The resulted fluidic did not undergo any internal sterilization post-fabrication. Previous work demonstrated the design and implementation of a ,macrofluidic device for the cultivation of bacteria with a peristaltic pump, a controlled heater and real-time optical sensing [57].…”

Section: Fabrication Back Boardmentioning

confidence: 99%

Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat

Gome,

Chak,

Tawil

et al. 2024

Preprint

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“…Research has been carried out to develop a large range of low-cost and specialized bioreactors. Some examples include human incubators [42], bioreactors for carrying out field studies in remote or underserved communities [43], laser-cut bioincubators for optogenetic bacterial culture [44], 3D printed microfluidic valves, DNA assemblers and open-source design heuristics [30, 45, 46], flexible cell incubators for affordable, safe, and sterile, hands-on experimentation with live microorganisms [47], microfluidic bioreactors for live-organoid imaging [48] and hypoxia chambers for cell culture [49]. While these reactor designs are breaking barriers in significant ways, there are no open-source and multipurpose devices that can enable researchers, communities and students to run multiple biosensing-related experiments in a portable and low-cost way.…”

Section: Introductionmentioning

confidence: 99%

MAIA: An open-source, modular, bioreactor for cities

Rico,

Kong,

Larson

2024

Preprint

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This work presents the design and fabrication of MAIA, an open-source, modular, low-cost, and portable bioreactor for democratizing the development of synthetic biology based projects for urban settings. The integration of open-source synthetic biology (synbio) tools in a city's infrastructure planning and design is crucial for addressing the great challenges related to urbanization. Synbio tools have great potential to help us complement our current sensing and actuating urban infrastructure. The MAIA reactor controls bacterial growth variables, making it suitable for cell-based experiments while reducing the need for expensive laboratory equipment. Its low-cost and open-source design allow for easy replication and modification, making it accessible to a broader audience. Its portability makes it suitable for use outside of traditional laboratory settings. We qualitatively and quantitatively validated the reactor's capability to support cell growth, stimulate gene expression, and act as a creative tool for students and users.

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BioBlocksLab: A portable DIY Bio Lab using BioBlocks language

Méndez‐Merino

Uría-Regojo

et al. 2023

Methods

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My First Biolab

Cited by 31 publications

References 9 publications

Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat

Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat

MAIA: An open-source, modular, bioreactor for cities

BioBlocksLab: A portable DIY Bio Lab using BioBlocks language

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