MYB pathways that regulate UV-B-induced anthocyanin biosynthesis in blueberry (Vaccinium corymbosum)

Song, Yan; Ma, Bin; Guo, Qixin; Zhou, Lianxia; Zhou, Xintong; Ming, Ziqing; You, Honglin; Zhang, Chunyu

doi:10.3389/fpls.2023.1125382

Cited by 10 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The groups IV and V contain one or two B-box domains without CCT domain. At same time, VcBBX21 , VcBBX24 , and VcBBX30-32 response to UV-B radiation, and UV-B radiation involve in color of flower and fruit [ 48 ]. In apple, MdBBX20, MdBBX22, MdBBX33 and MdBBX54 of group IV with two B-box domains without CCT domain regulate UV-B-induced anthocyanin biosynthesis and MdBBX37 of group V with one B-box domain without CCT domain also regulates anthocyanin biosynthesis [ 20 , 22 – 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…In pear, PpBBX16, PpBBX18 and PpBBX21, with two B-box domains without CCT domain, regulate anthocyanin accumulation [ 59 , 60 ]. The previous study showed that VcBBX30 and VcBBX32 co-expressed with the possible anthocyanin biosynthesis gene VcMYBA2 and VcMYB114 under UV-B radiation [ 48 ]. Thus, VcBBX21 , VcBBX24 , and VcBBX30-32 , especially VcBBX30 and VcBBX32 , may regulate UV-B-induced anthocyanin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…The predicted protein interaction networks based on orthologous BBXs of Arabidopsis showed that VcBBX18–25 interact with HY5, HY5 HOMOLOG (HYH), and COP1. The previous study showed that VcBBX30 and VcBBX32 co-expressed with the VcHY5, and VcHY5 co-expressed with COP1 under UV-B radiation [ 48 ]. Moreover, VcBBX21 , VcBBX24 , VcBBX30 , and VcBBX32 were regulated by UV-B radiation.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Genome-wide analysis of blueberry B-box family genes and identification of members activated by abiotic stress

Liu,

Sun,

et al. 2023

BMC Genomics

Self Cite

View full text Add to dashboard Cite

Background B-box (BBX) proteins play important roles in regulating plant growth, development, and abiotic stress responses. BBX family genes have been identified and functionally characterized in many plant species, but little is known about the BBX family in blueberry (Vaccinium corymbosum). Result In this study, we identified 23 VcBBX genes from the Genome Database for Vaccinium (GDV). These VcBBXs can be divided into five clades based on gene structures and conserved domains in their encoded proteins. The prediction of cis-acting elements in the upstream sequences of VcBBX genes and protein–protein interactions indicated that VcBBX proteins are likely involved in phytohormone signaling pathways and abiotic stress responses. Analysis of transcriptome deep sequencing (RNA-seq) data showed that VcBBX genes exhibited organ-specific expression pattern and 11 VcBBX genes respond to ultraviolet B (UV-B) radiation. The co-expression analysis revealed that the encoded 11 VcBBX proteins act as bridges integrating UV-B and phytohormone signaling pathways in blueberry under UV-B radiation. Reverse-transcription quantitative PCR (RT-qPCR) analysis showed that most VcBBX genes respond to drought, salt, and cold stress. Among VcBBX proteins, VcBBX24 is highly expressed in all the organs, not only responds to abiotic stress, but it also interacts with proteins in UV-B and phytohormone signaling pathways, as revealed by computational analysis and co-expression analysis, and might be an important regulator integrating abiotic stress and phytohormone signaling networks. Conclusions Twenty-three VcBBX genes were identified in blueberry, in which, 11 VcBBX genes respond to UV-B radiation, and act as bridges integrating UV-B and phytohormone signaling pathways according to RNA-seq data. The expression patterns under abiotic stress suggested that the functional roles of most VcBBX genes respose to drought, salt, and cold stress. Our study provides a useful reference for functional analysis of VcBBX genes and for improving abiotic stress tolerance in blueberry.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide analysis of blueberry B-box family genes and identification of members activated by abiotic stress

Liu,

Sun,

et al. 2023

BMC Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, the expression of MYBA1 and MYBA2 genes (subgroup 6) were upregulated under exogenous ABA treatment (Figure 5). In which, the MYBA2 gene expression was upregulated, was positively correlated with the expression of ASGs under UV-B radiation and played an important role in the UV-B-induced anthocyanin biosynthetic pathway [70]. MYBA1 has been reported as VcMYB437and the expression of VcMYB437 was higher in the skin of ripe blueberry fruit than in the pulp, implying the potential roles in anthocyanin biosynthesis [71].…”

Section: The Regulatory Network Of Aba-induced Blueberry Anthocyanin ...mentioning

confidence: 98%

Exogenous Abscisic Acid Regulates Anthocyanin Biosynthesis and Gene Expression in Blueberry Leaves

Ma,

Song,

Feng

et al. 2024

Horticulturae

Self Cite

View full text Add to dashboard Cite

Blueberry (Vaccinium corymbosum) leaves have a positive influence on health because of their phenolic contents, including anthocyanins. Phytohormone abscisic acid (ABA) promotes anthocyanin accumulation, but the underlying mechanisms are unclear in blueberry leaves. In this study, we found that exogenous ABA promotes anthocyanin accumulation in blueberry leaves and we explored the global molecular events involved in these physiological changes by treating in vitro-grown blueberry seedlings with ABA and performing transcriptome deep sequencing (RNA-seq). We identified 6390 differentially expressed genes (DEGs), with 2893 DEGs at 6 h and 4789 at 12 h of ABA treatment compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to plant hormone signal transduction and phenylpropanoid and flavonoid biosynthesis were significantly enriched at both stages of the ABA treatment. Analysis of DEGs in plant hormone signal transduction pathways revealed that exogenous ABA affected the expression of genes from other plant hormone signaling pathways, especially brassinosteroid, auxin, and gibberellin signaling. To elucidate the mechanism driving anthocyanin biosynthesis in blueberry in response to ABA treatment, we screened anthocyanin biosynthesis structural genes (ASG) from the phenylpropanoid and flavonoid biosynthetic pathways, MYB transcription factor genes from R2R3-MYB subgroups 5, 6, and 7 and ABRE-binding factor (ABF) genes from the ABA signal transduction pathway. Pearson’s correlation coefficient (r) analysis indicated that the ABFs, MYBs, and structural genes form a network to regulate ABA-induced anthocyanin biosynthesis and MYBA1 is likely to play an important role in this regulatory network. These findings lay the foundation for improving anthocyanin biosynthesis in blueberry leaves.

show abstract

“…As MYB12 and MYB111 have been proven to play a crucial role in the production of flavonol glycoside in response to UV-B radiation [41,42], we further explore the sequence and expression pattern of LcMYB12 and LcMYB111 identified by the above WGCNA analysis. Firstly, a phylogenetic tree was constructed for LcMYB111 and LcMYB12 with other MYB111s, which could be up-regulated by UV-B radiation, including AtMYB111, AtMYB12, HaMYB111 [43], CsMYB12 [44], VcMYB114 [45], PpMYB-FL [46] and VviMYBF1 [47]. Phylogenetic analysis revealed that LcMYB111 clustered together with AtMYB111, while LcMYB12 clustered together with AtMYB12 (Figure 7A), indicating that they are homologs of AtMYB111 and AtMYB12 in litchi.…”

Section: Key R2r3-myb Transcription Factors Up-regulated By Uv-b Radi...mentioning

confidence: 99%

Metabolome and Transcriptome Analysis Provide Insights into Flower Bud Color Variation in the Adaptation to UV-B Radiation of Litchi

Xiao,

Wang,

Jiang

et al. 2024

Agronomy

View full text Add to dashboard Cite

Flower color variations have increasingly been recognized as playing an important role in the adaptation to UV-B radiation; however, the underlying mechanism is poorly understood in perennial fruit trees. Litchi is an important fruit tree, and extremely early maturing (EEM) and middle-to-late-maturing (MLM) cultivars that originated from regions with high and low UV-B radiation have dark brown and light yellow flower buds, respectively, while their hybrid early-maturing (EM) cultivars have an intermediate brown flower bud. This study comprehensively analyzed the metabolome and transcriptome of flower buds of litchi EEM, EM and MLM cultivars to explore the mechanism underlying flower color variation during the adaptation to UV-B radiation for the first time. Metabolomic analysis identified 72 flavonoids in litchi flower buds, among which a higher accumulation of flavonol glycosides was responsible for darker flower buds of EEM cultivars. And transcriptome analysis revealed key structural genes, including LcCHI, LcFLS and seven UGTs, together with two transcription factors (LcMYB12 and LcMYB111), which could be directly up-regulated by UV-B radiation, playing critical roles in regulating the differential accumulation of flavonol glycosides. These results provide new insights into the molecular mechanism underlying adaptation to UV-B radiation and provide a genetic basis for future breeding of stress-tolerant cultivars of litchi.

show abstract

MYB pathways that regulate UV-B-induced anthocyanin biosynthesis in blueberry (Vaccinium corymbosum)

Cited by 10 publications

References 49 publications

Genome-wide analysis of blueberry B-box family genes and identification of members activated by abiotic stress

Genome-wide analysis of blueberry B-box family genes and identification of members activated by abiotic stress

Exogenous Abscisic Acid Regulates Anthocyanin Biosynthesis and Gene Expression in Blueberry Leaves

Metabolome and Transcriptome Analysis Provide Insights into Flower Bud Color Variation in the Adaptation to UV-B Radiation of Litchi

Contact Info

Product

Resources

About