MYB89 Transcription Factor Represses Seed Oil Accumulation

Li, Dong; Jin, Changyu; Duan, Shengwen; Zhu, Yu; Qi, Shihua; Liu, Kaige; Gao, Chenhao; Ma, Hongwei; Zhang, Meng; Liao, Yuncheng; Chen, Mingxun

doi:10.1104/pp.16.01634

Cited by 100 publications

(64 citation statements)

References 88 publications

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“…Studies have shown that some miRNAs and their target genes are related to seed size [13,46], and most of the target genes belong to transcription factors, mainly ARF [19,47], CNR [48] and MYB [49]. Our data showed there were 23 miRNA-mRNA Because MYB89 transcription factor represses seed oil accumulation in Arabidopsis, and it was expressed predominantly in developing seeds during maturation [50].…”

Section: Identification Of Mirnas In Developing Seeds Of Tea Oil Camementioning

confidence: 55%

Identification of miRNA-mRNA regulatory modules involved in lipid metabolism and seed development in a woody-oil tree (Camellia oleifera)

Wu¹,

Ruan²,

Shah³

et al. 2020

Preprint

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BackgroundTea oil camellia ( Camellia oleifera ), an important woody oil tree, is a source of seed oil of high nutritional and medicinal values and has been being widely planted in southern China. However, there is no report on the identification of miRNAs involved in lipid metabolism and seed development in high- and low-oil cultivars of tea oil camellia. Thus, we explored the roles of miRNAs in the critical period of oil formation and accumulation in tea oil camellia, and identified miRNA-mRNA regulatory modules involved in lipid metabolism and seed development. ResultsSixteen small RNA libraries for high- and low-oil cultivars of the critical period of oil biosynthesis were constructed. A total of 196 miRNAs, including 156 known miRNAs from 35 families and 40 novel miRNAs, were identified, and 55 significantly differentially expressed miRNAs were found, which included 34 up-regulated miRNAs and 21 down-regulated miRNAs. An integrated analysis of miRNA and mRNA transcriptome sequence data and qRT-PCR-based information was performed and revealed that nine miRNA-mRNA regulatory modules were related to lipid metabolism, such as the negative regulatory modules of ath-miR858b- MYB82 / MYB3 / MYB44 represses seed oil biosynthesis and a positive regulation module of csi-miR166e-5p- S-ACP - DES6 for formation and accumulation of oleic acid. Twenty-tree miRNA-mRNA regulatory modules were involved in the regulation of seed size, such as a negative regulatory module of hpe-miR162a_L-2- ARF19 involved in early seed development. Twelve miRNA-mRNA regulatory modules regulating growth and development were identified, such as the negative regulatory modules of han-miR156a_L+1- SPL4 / SBP2 promoting early seed development. The targeting relationship of the cpa-miR393_R-1-AFB2 regulatory module were verified by luciferase activity assays. ConclusionMultiple microRNAs (miRNAs) were identified to involve in developing seeds of tea oil camellia, especially discovering several miRNA-mRNA regulatory modules involving in seed development and lipid metabolism. These data provide important theoretical value and a scientific basis for the genetic improvement of new varieties of tea oil camellia in the future.

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Section: Identification Of Mirnas In Developing Seeds Of Tea Oil Camementioning

confidence: 55%

Identification of miRNA-mRNA regulatory modules involved in lipid metabolism and seed development in a woody-oil tree (Camellia oleifera)

Wu¹,

Ruan²,

Shah³

et al. 2020

Preprint

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“…MYB89 (R2R3‐MYB transcription factor) highly expresses in developing seed during maturation which acts as a repressor for oil accumulation in seeds. The knockout of MYB89 factor resulted into high oil accumulation in myb89‐1 mutants in Arabidopsis (Li et al , ). Araip.22PIW (acyl‐CoA synthetase) serving as the carbon source for fatty acid biosynthesis in Arabidopsis which triggers oil accumulation in seed therefore might be associated with seed mass (Lin and Oliver, ).…”

Section: Discussionmentioning

confidence: 99%

Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut (Arachis hypogaea)

Gangurde

Wang

Shasidhar

et al. 2019

Plant Biotechnology Journal

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email baozhu.guo@usda.gov (B. G.); Tel 91-40-30713345; fax 91-40-30713074; email r.k.varshney@cgiar.org (R. K. V.)) † These authors contributed equally to this study. SummaryMultiparental genetic mapping populations such as nested-association mapping (NAM) have great potential for investigating quantitative traits and associated genomic regions leading to rapid discovery of candidate genes and markers. To demonstrate the utility and power of this approach, two NAM populations, NAM_Tifrunner and NAM_Florida-07, were used for dissecting genetic control of 100-pod weight (PW) and 100-seed weight (SW) in peanut. Two high-density SNP-based genetic maps were constructed with 3341 loci and 2668 loci for NAM_Tifrunner and NAM_Florida-07, respectively. The quantitative trait locus (QTL) analysis identified 12 and 8 major effect QTLs for PW and SW, respectively, in NAM_Tifrunner, and 13 and 11 major effect QTLs for PW and SW, respectively, in NAM_Florida-07. Most of the QTLs associated with PW and SW were mapped on the chromosomes A05, A06, B05 and B06. A genomewide association study (GWAS) analysis identified 19 and 28 highly significant SNP-trait associations (STAs) in NAM_Tifrunner and 11 and 17 STAs in NAM_Florida-07 for PW and SW, respectively. These significant STAs were co-localized, suggesting that PW and SW are co-regulated by several candidate genes identified on chromosomes A05, A06, B05, and B06. This study demonstrates the utility of NAM population for genetic dissection of complex traits and performing highresolution trait mapping in peanut.

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“…Total RNA extraction and first‐strand cDNA synthesis were conducted as previously described (Li et al . ). Gene expression was determined by quantitative reverse transcription PCR (qRT‐PCR), using three independent biological replicates, with two technical replicates for each biological replicate.…”

Section: Methodsmentioning

confidence: 97%

Brassica napusGLABRA3‐1 promotes anthocyanin biosynthesis and trichome formation in true leaves when expressed in Arabidopsis thaliana

et al. 2017

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Previous studies have shown that GLABRA3 (AtGL3), a bHLH transcription factor, plays essential roles in anthocyanin biosynthesis and trichome formation in Arabidopsis thaliana. However, there have been no such studies of a homologue, BnGL3, from the closely related crop, Brassica napus. Here, we analysed the BnGL3-1 coding domain sequence from the B. napus cultivar QINYOU Seven, identified conserved protein domains and performed a phylogenetic analysis to elucidate its relationship with homologues form a range of plant species. When expressed in tobacco leaves as a fusion protein with green fluorescent protein, BnGL3-1 accumulated in the nucleus, consistent with its predicted function as a transcription factor. Ectopic expression of the BnGL3-1 gene in the A. thaliana gl3-3 mutant resulted in levels of anthocyanins and numbers of trichomes in true leaves that were higher than in wild-type plants. Moreover, overexpression of BnGL3-1 in gl3-3 compensated for the promotion and repression of genes involved in anthocyanin biosynthesis and trichome formation, respectively, that has been reported in gl3-3 young shoots and expanding true leaves. This study provides new insights into GL3 function in anthocyanin biosynthesis and trichome formation in crucifers, and represents a promising target for genetic manipulation of B. napus.

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MYB89 Transcription Factor Represses Seed Oil Accumulation

Cited by 100 publications

References 88 publications

Identification of miRNA-mRNA regulatory modules involved in lipid metabolism and seed development in a woody-oil tree (Camellia oleifera)

Identification of miRNA-mRNA regulatory modules involved in lipid metabolism and seed development in a woody-oil tree (Camellia oleifera)

Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut (Arachis hypogaea)

Brassica napusGLABRA3‐1 promotes anthocyanin biosynthesis and trichome formation in true leaves when expressed in Arabidopsis thaliana

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