MYC genes with differential responses to tapping, mechanical wounding, ethrel and methyl jasmonate in laticifers of rubber tree (Hevea brasiliensis Muell. Arg.)

Zhao, Yue; Zhou, Limin; Chen, Yueyi; Yang, Shuguang; Weimin, Tian

doi:10.1016/j.jplph.2011.02.010

Cited by 42 publications

(28 citation statements)

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“…Higher MYC protein expression could then result in an increased rate of apoptosis in tumors with HER2 protein overexpression. Alternatively, trastuzumab was recently shown to inhibit glycolysis in HER2+ cells (42), and down-regulation of MYC protein has been shown to contribute to cancer cell survival under dual deficiency of oxygen and glucose (43). Conceivably, when MYC protein is overexpressed in HER2+ tumors that are treated with trastuzumab, the protective effect of MYC down-regulation on cancer cell survival in low glucose settings would be lost.…”

Section: Discussionmentioning

confidence: 99%

Impact of c-MYC Protein Expression on Outcome of Patients with Early-Stage HER2+ Breast Cancer Treated with Adjuvant Trastuzumab NCCTG (Alliance) N9831

Dueck

Reinholz

Geiger

et al. 2013

Clinical Cancer Research

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Purpose This study investigated the association between tumor MYC protein expression and disease-free survival (DFS) of patients randomized to receive chemotherapy alone (Arm A) or chemotherapy with sequential (Arm B) or concurrent trastuzumab (Arm C) in the N9831 (Alliance) adjuvant HER2+ trastuzumab breast cancer trial. Patients/Methods This analysis included 1736 patients randomized to Arms A, B, and C on N9831. Nuclear MYC protein expression was determined in tissue microarray (TMA) sections containing three biopsies per patient or whole tissue sections (WS) using standard immunohistochemistry (clone 9E10). A tumor was considered positive for MYC protein overexpression (MYC+) if the nuclear 3+ staining percentage was >30%. Results 574 (33%) tumors were MYC+. MYC+ was associated with hormone receptor positivity (χ2 p=0.006), tumors ≥ 2 cm (χ2 p=0.02), and a higher rate of nodal positivity (χ2 p<0.001). Hazard ratios (HRs) for DFS (median follow-up: 6.1 years) for Arm C versus A were 0.52 (p=0.006) and 0.65 (p=0.006) for patients with MYC+ and MYC- tumors, respectively (interaction p=0.40). For Arm B versus A, HRs for patients with MYC+ and MYC- tumors were 0.79 (p=0.21) and 0.74 (p=0.04), respectively (interaction p=0.71). For Arm C versus B, HRs for patients with MYC+ and MYC- tumors were 0.56 (p=0.02) and 0.89 (p=0.49), respectively (interaction p=0.17). Conclusions Our data do not support an impact of tumor MYC protein expression on differential benefit from adjuvant trastuzumab.

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Section: Discussionmentioning

confidence: 99%

Impact of c-MYC Protein Expression on Outcome of Patients with Early-Stage HER2+ Breast Cancer Treated with Adjuvant Trastuzumab NCCTG (Alliance) N9831

Dueck

Reinholz

Geiger

et al. 2013

Clinical Cancer Research

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“…Unlike myrosin cells, the molecular mechanisms regulating S-cell development remain elusive, largely due to a paucity of gene markers for S-cells. Recently, transcripts of the rubber tree ( Hevea brasiliensis ) subgroup 3E-like bHLH genes, HblMYC1 and HblMYC2 , were found to increase or decrease, respectively, during mechanical wounding or JA treatment of laticifer cells, suggesting that these transcription factors may participate in JA-induced production of latex (Zhao et al, 2011). Parallels between laticifer and S-cell development suggest that S-cell development in Arabidopsis may also be regulated by the same subgroup 3E bHLHs that regulate glucosinolate biosynthesis.…”

Section: Developmental Pathway Regulationmentioning

confidence: 99%

Regulation of plant secondary metabolism and associated specialized cell development by MYBs and bHLHs

2016

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Plants are unrivaled in the natural world in both the number and complexity of secondary metabolites they produce, and the ubiquitous phenylpropanoids and the lineage-specific glucosinolates represent two such large and chemically diverse groups. Advances in genome-enabled biochemistry and metabolomic technologies have greatly increased the understanding of their metabolic networks in diverse plant species. There also has been some progress in elucidating the gene regulatory networks that are key to their synthesis, accumulation and function. This review highlights what is currently known about the gene regulatory networks and the stable sub-networks of transcription factors at their cores that regulate the production of these plant secondary metabolites and the differentiation of specialized cell types that are equally important to their defensive function. Remarkably, some of these core components are evolutionarily conserved between secondary metabolism and specialized cell development and across distantly related plant species. These findings suggest that the more ancient gene regulatory networks for the differentiation of fundamental cell types may have been recruited and remodeled for the generation of the vast majority of plant secondary metabolites and their specialized tissues.

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“…Currently, there are only 37,745 rubber tree ESTs available in the national center for biotechnology information (NCBI) database (as of Dec 2011). Most of these ESTs were generated with the aim to identify the candidate genes involved in various abiotic and biotic stress responses and rubber biosynthesis [7-20], whereas only superoxide dismutase was further studied by using transgenic approaches [21-23]. Among these 37,745 ESTs, most were derived from the latex, and only a few from the bark and leaf.…”

Section: Introductionmentioning

confidence: 99%

De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis Muell. Arg.)

et al. 2012

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BackgroundIn rubber tree, bark is one of important agricultural and biological organs. However, the molecular mechanism involved in the bark formation and development in rubber tree remains largely unknown, which is at least partially due to lack of bark transcriptomic and genomic information. Therefore, it is necessary to carried out high-throughput transcriptome sequencing of rubber tree bark to generate enormous transcript sequences for the functional characterization and molecular marker development.ResultsIn this study, more than 30 million sequencing reads were generated using Illumina paired-end sequencing technology. In total, 22,756 unigenes with an average length of 485 bp were obtained with de novo assembly. The similarity search indicated that 16,520 and 12,558 unigenes showed significant similarities to known proteins from NCBI non-redundant and Swissprot protein databases, respectively. Among these annotated unigenes, 6,867 and 5,559 unigenes were separately assigned to Gene Ontology (GO) and Clusters of Orthologous Group (COG). When 22,756 unigenes searched against the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG) database, 12,097 unigenes were assigned to 5 main categories including 123 KEGG pathways. Among the main KEGG categories, metabolism was the biggest category (9,043, 74.75%), suggesting the active metabolic processes in rubber tree bark. In addition, a total of 39,257 EST-SSRs were identified from 22,756 unigenes, and the characterizations of EST-SSRs were further analyzed in rubber tree. 110 potential marker sites were randomly selected to validate the assembly quality and develop EST-SSR markers. Among 13 Hevea germplasms, PCR success rate and polymorphism rate of 110 markers were separately 96.36% and 55.45% in this study.ConclusionBy assembling and analyzing de novo transcriptome sequencing data, we reported the comprehensive functional characterization of rubber tree bark. This research generated a substantial fraction of rubber tree transcriptome sequences, which were very useful resources for gene annotation and discovery, molecular markers development, genome assembly and annotation, and microarrays development in rubber tree. The EST-SSR markers identified and developed in this study will facilitate marker-assisted selection breeding in rubber tree. Moreover, this study also supported that transcriptome analysis based on Illumina paired-end sequencing is a powerful tool for transcriptome characterization and molecular marker development in non-model species, especially those with large and complex genomes.

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MYC genes with differential responses to tapping, mechanical wounding, ethrel and methyl jasmonate in laticifers of rubber tree (Hevea brasiliensis Muell. Arg.)

Cited by 42 publications

References 88 publications

Impact of c-MYC Protein Expression on Outcome of Patients with Early-Stage HER2+ Breast Cancer Treated with Adjuvant Trastuzumab NCCTG (Alliance) N9831

Impact of c-MYC Protein Expression on Outcome of Patients with Early-Stage HER2+ Breast Cancer Treated with Adjuvant Trastuzumab NCCTG (Alliance) N9831

Regulation of plant secondary metabolism and associated specialized cell development by MYBs and bHLHs

De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis Muell. Arg.)

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