MYC Promoted The Formation of RANKL Induced Osteoclasts By Modulating The miR-320a/PTEN Axis

Abstract

Background: Osteoporosis is a serious bone metabolism disease. Recent studies have shown that MYC could promote the formation of osteoclasts. There is study has also shown that miR-320a could cause the damage of osteoblast by inducing oxidative stress. By querying the database, we found that MYC has the potential to target and affect the expression of miR-320a. However, the effect of MYC and miR-320a on the RANKL induced osteoclasts is unclear.Results: MYC promoted the expression of miR-320a in RAW 264.7 cells by binding on the promoter of miR-320a. Inhibition of MYC and miR-320a suppressed the formation of RANKL induced osteoclasts by inhibiting the expression of c-Fos, NFATc1, TRAP and CTSK. Moreover, the expression of c-Fos, NFATc1, TRAP and CTSK was rescued and the RANKL induced osteoclasts was promoted after the repressing the expression of PTEN.Conclusions: MYC enhanced the formation of RANKL induced osteoclasts by modulate the miR-320a/PTEN pathway.