Mycelial biomass and intracellular polysaccharides yield of edible mushroom Agaricus blazei produced in wheat extract medium

Zhai, Fei-Hong; Han, Jihong

doi:10.1016/j.lwt.2015.10.010

Cited by 4 publications

(5 citation statements)

References 21 publications

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“…The dry mycelium coarse powder was extracted for its metabolites using ethanol and chloroform separately. 30,31 In 50ml of solvent 10g of mycelium powder were added and left on a rotary shaker at 150 rpm for 48 hrs. 32 The extracts were filtered after 48 hrs and the solvent was allowed to evaporate thus leaving behind a thick paste which was stored in glass vials at 4°C until further use.…”

Section: Metabolite Extraction and Fermentationmentioning

confidence: 99%

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

Patankar¹,

Reshi²

2023

J. Pure Appl. Microbiol.

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In the current study, fungi from the mangrove ecosystem of Mumbai were isolated and their metabolites were screened for antibacterial potential. Two weeks old broth and mycelium were extracted using chloroform and methanol. Antibacterial property of solvent extracts was evaluated at various concentrations (2 – 10 µg/ml) against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumonia and Escherichia coli, by well diffusion method. Fungi isolated were identified as Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Cylindrocladium scoparium and Colletotrichum wuxiense. Results revealed that broth solvent extracts of isolates inhibited the growth of all gram-positive test bacteria, chloroform broth extract of Cylindrocladium scoparium, Colletotrichum wuxiense and ethanolic broth extract of Aspergillus flavus, Trichoderma harzianum exhibited antibacterial potential against gram negative test organisms. Chloroform and ethanol mycelium extracts of Trichoderma harzianum and Aspergillus flavus, respectively, exhibited 100% growth inhibition potential against all test organisms. The current investigation endorses the potent secondary metabolism of the identified isolates and their potential to synthesise antibacterial compounds.

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Section: Metabolite Extraction and Fermentationmentioning

confidence: 99%

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

Patankar¹,

Reshi²

2023

J. Pure Appl. Microbiol.

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“…Centrifuging the fermentation substrate at 8000 rpm for 12 min and then drying at 85 °C to constant weight to detect the biomass [13]. Te production of EPS detected according to the phenol-sulfuric method [13,19].…”

Section: Determination Of Mycelial Biomass and Eps Yieldmentioning

confidence: 99%

“…At present, RSM is becoming more and more popular in optimizing the medium nutrition components. Zhai and Han [13] reported that after optimizing the medium with RSM, the biomass and intracellular polysaccharides production of Agaricus blazei was increased. Also, Liu et al [19] and Dwibedi et al [25] reported that RSM would be an efective method to optimize the medium and provide optimum condition for fungi culturing.…”

Section: Optimization Of Biomass and Eps Production By Rsmmentioning

confidence: 99%

“…Compared with the cultivation of fruit bodies, submerged culture has many advantages, such as confned space, shorter time, easier control, and higher content of bioactive metabolites [12]. Among the process of fermentation, nitrogen and carbon sources of medium had an important role in promoting the growth of mycelia [13,14]. Many reports showed that the natural materials have the potential to be substrate of fungal medium [10][11][12][13], which gives us enlightenment that natural materials may have the potential ability to cultivate H. lacunosa.…”

Section: Introductionmentioning

confidence: 99%

“…Among the process of fermentation, nitrogen and carbon sources of medium had an important role in promoting the growth of mycelia [13,14]. Many reports showed that the natural materials have the potential to be substrate of fungal medium [10][11][12][13], which gives us enlightenment that natural materials may have the potential ability to cultivate H. lacunosa. On the other hand, fermentation parameters (such as, medium volume, fermentation time and temperature, pH in medium, and rotation speed) also have important efects on the production of polyphenolics, polysaccharide, etc [15].…”

Section: Introductionmentioning

confidence: 99%

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Statistical Optimization of Biomass and Extracellular Polysaccharide Production by the Wild Helvella lacunosa Mycelium in Liquid-State Fermentation Using a Pine Needles Extract Medium

Guo

et al. 2023

Journal of Chemistry

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The objective of this study was using a statistical model to optimize the medium formula and liquid culturing conditions, which was employed to evaluate the potency of pine needles extract as nutritional component in basal medium for culturing Helvella lacunose in order to enhancing the production of mycelia and extracellular polysaccharides (EPS). The most proper carbon and nitrogen source, various fermentation parameters (i.e., initial pH in medium, fermentation time, and temperature) were investigated by single factor test. Based on the experimentation, the optimal concentrations of carbon and nitrogen sources were analyzed by response surface methodology (RSM). The results showed that the optimal medium formula and liquid culturing conditions were 1000 mL pine needles extract, 33.56 g glucose, 4.11 g peptone, 4.06 g YE, 1 g KH2PO4, 1 g NaHCO3, 0.5 g MgSO4, 0.01 g vitamin B1 for the accumulation of mycelial biomass (at pH 6.0, 25°C culturing of 7 days), and EPS (at pH 7.0, 25°C culturing of 10 days). Under optimized medium, the experimental production of mycelia and EPS were 11.78 g/L and 223.16 mg/L, which were 10.52 and 4.85 times than that of control (1.12 ± 0.52 g/L and 46.08 ± 1.18 mg/L, respectively). This data implied that statistical model is very useful tools for optimizing media components and fermentation parameters, and the screened media components and parameters have reference value for further large-scale production.

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Chemical features and bioactivity of grain flours colonized by macrofungi as a strategy for nutritional enrichment

et al. 2019

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Mycelial biomass and intracellular polysaccharides yield of edible mushroom Agaricus blazei produced in wheat extract medium

Cited by 4 publications

References 21 publications

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

Statistical Optimization of Biomass and Extracellular Polysaccharide Production by the Wild Helvella lacunosa Mycelium in Liquid-State Fermentation Using a Pine Needles Extract Medium

Chemical features and bioactivity of grain flours colonized by macrofungi as a strategy for nutritional enrichment

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