MYCELIAL GROWTH AND SPORULATION OF Fusarium spp. PATHOGENIC TO Ilex paraguariensis IN DIFFERENT CULTURE MEDIA AND UNDER EXPOSURE TO DIFFERENT LIGHT LEVELS.

Mezzomo, Ricardo; Rolim, Jessica Mengue; Poletto, Tales; Oliveira, Marília Boff de; Lazarotto, Marília; Muniz, Marlove Fátima Brião

doi:10.5380/rsa.v19i1.55844

Cited by 7 publications

(8 citation statements)

References 3 publications

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“…dianthi studied on different solid media indicated that, potato dextrose agar, Richards's agar, Czapek's Dox agar, and Oat meal agar supported the maximum growth of the fungal colony. In another study, the V8 and malt extract agar culture media exhibited the maximum diameter of the fungal (F. oxysporum and F. solani) colonies, while the malt extract agar and potato sucrose agar media contributed to the highest sporulation of Fusarium spp (Mezzomo et al, 2018).…”

Section: Effect Of Culture Media On the Mycelial Growth Of F Solanimentioning

confidence: 91%

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Mallik

Sikder

Hossain

et al. 2022

Int. J. Agril. Res. Innov. & Tech.

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Fruit rot disease of sweet pepper is one of the main fungal diseases causing huge economic losses to the grower. An experiment was conducted to find out the fungal pathogen associated with fruit rot disease of sweet pepper, obtained from experimental fields of Jahangirnagar University, Bangladesh. Fruit rot disease-causing fungus was isolated from infected fruits and identified using morphological characterization based on colony features, mycelia, conidia as well as molecular characterization based on internal transcribe spacer (ITS) region of the fungus. ITS sequence of our studied fungus MH368146.1 was genetically 99-100% similar to sequences of Fusarium solani in NCBI database. Typical fruit rot symptoms were reproduced by artificial inoculations of the isolated fungus. The mycelial growth of this fungus was evaluated on ten different solid culture media i.e., Potato Dextrose Agar, Yeast Extract Agar, Honey Peptone Agar, Hansen’s Medium, Sabouraud’s Glucose Agar, Kauffman’s Agar, Potato Sucrose Agar, Richard’s Agar and Carrot Agar. Fungus grew well on all tested solid culture media. Several bio-control agents and two commercial fungicides were evaluated against isolated fungus under in vitro condition, in which the highest percent inhibition of radial growth of the fungus was determined as 64.75% due to Trichoderma reesei isolate 2, and 60.63% by Tilt 250 EC (500 ppm) at 7 days post-incubation. Therefore, T. reesei was found as the most suitable to control the growth of F. solani under laboratory conditions. However, further pot and field trials needed to be confirmed the bio-control potential of it. Int. J. Agril. Res. Innov. Tech. 11(2): 108-116, Dec 2021

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Section: Effect Of Culture Media On the Mycelial Growth Of F Solanimentioning

confidence: 91%

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Mallik

Sikder

Hossain

et al. 2022

Int. J. Agril. Res. Innov. & Tech.

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“…Growth characters of F. oxysporum f. sp. gerberae studied on different solid media indicated that the growth was maximum on Oat meal agar followed by Richards's agar, Czapek's Dox agar, and Potato Dextrose agar supported maximum growth of fungal colony (Rajirani et al, 2000;Rajirani 2001;Chittem and Kulkarni, 2008;Mezzomo et al, 2018;Westphal et al, 2021). These studies revealed that among the solid substrates, leafy substrates, bran and oil seed cakes, and the liquid substrates tested mature coconut water supported maximum biomass and macrconidia.…”

Section: Effect Of Different Mediamentioning

confidence: 97%

Fusarium pallidoroseum: A potential entomopathogenic agent for the biological management of Aphis gossypii

Srivastava

Shukla

2021

JANS

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With rising need of switching over to sustainable agricultural practices, utilization of entomopathogenic fungi (EPF) as biocontrol agents, provides better substitute against chemical pesticides- having several side-effects. Therefore, an attempt have been made to explore the potential EPF fungi that could be incorporated into IPM practices for control of Helicoverpa armigera Hubner and Aphis gossypii Glover. Regarding this, an entomopathogenic fungus, Fusarium pallidoroseum (Cooke) Sacc, was isolated from natural population of H. armigera infesting chickpea (Cicer arietinum L.) and explored efficacy under in-vitro & field conditions. The findings of present investigation shows efficacy of F. pallidoroseum as potential biocontrol agent against okra aphid (A. gossypii), as it inflicted initial mortality of 43.33% nymphs on 2nd day and lead to complete annihilation (93.33%) of nymph population on 8th day of spore suspension application at 1x1010 spores/mL concentration. The observations against adult okra aphid clearly demonstrated that spraying of 1 x 1010 spores/mL of F. pallidoroseum resulted 66.67% mortality after 8th days of spraying. Increased mortality was recorded with increase in spore suspension concentrations. The LC50 & LC90 value for F. pallidoroseum against nymphs of A. gossypii was recorded 3.79 x 105 and 2.74 x 108, respectively. The findings were used to develop formulations (1 × 104 to 1 × 1010 spore suspension/mL conc), and tested at field-level. The results showed that formulation at 1 x 1010 spores/mL conc was most effective against A. gossypii, recorded 93.33% mortality of nymphs & 66.67% mortality of adults; could be used under IPM practices.

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“…The influence of temperature on growth and sporulation of Fusarium sp isolates was observed by growing 5 mm colonies diameter in the middle of Potato Dextrose agar (PDA) medium, then grew at (10,16,22,28,34, and 40) ° C ± 1 °C. Growth diameter and sporulation were observed at 7 d after inoculation [9]. The growth rate on pH (5, 6, 7 and 8) was observed after 7 d cultured on PDB, but resistance to Fungicides was observed by measuring the colony diameter on PDA at 7 d after inoculation.…”

Section: In Vitro Isolates Resistance Test To Temperature Ph and Fumentioning

confidence: 99%

“…Most of the Fusarium oxysporum isolates favored the pH of 7.5, slightly alkaline conditions. Mezzomo et al [9] found that mycelia of F. oxysporum, which grew within the range of pH 2 to pH 12, pH 0 to pH 6, was the most suitable for the growth of all Fusarium species, while a highly acidic medium was unsuitable for the sporulation of all Fusarium species. Testing the sensitivity of the active ingredients of fungicides is done by adding fungicides commonly used by farmers at recommended dosages.…”

Section: Effect Of Growth Temperature Ph Fungicide On Fusarium Sp Gmentioning

confidence: 99%

“…Arcía et al [8] reported that Fusarium spp., F. oxysporum and F. solani require pH 4.5 and 6.0, while F. graminearum and F. equiseti at pH 3.5 and pH 6.5. That pH 5.5 to pH 7.0 was the optimum pH for growth and sporulation of F. solani [9]. Besides, Hsuan et al [10] tested Gibberella fujikuroi at various temperatures, suitable temperatures for growth and sporulation was a temperature of 30 °C followed by 25 °C, 20 °C, and 35 °C.…”

Section: Introductionmentioning

confidence: 98%

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Variability of Fusarium oxysporum f. sp. lycopersici from different altitudes in East Java, Indonesia

2021

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Fusarium wilt disease attacks the tomato plants, and Solanaceae plants with losses can reach 90 %. Thus, this disease was tough to control. The morphology and pathogenicity variation caused failure disease control. Both variations could be affected by the altitude of the planting site. Therefore, it also required special handling of management as well. The specific objectives of this study to investigate the Fusarium isolate from different altitude response of physical properties (acid-base pH, low-high temperature, and fungicides of other active ingredients) and growth rate of Fusarium. All Fusarium sp. were isolated from the leaves, soil, roots, and stem of infected tomato plants. Before a series of tests were carried out, all of the isolates were tested for pathogenicity, then tested for the influence of abiotic factors. This study obtained four isolates from high altitude, four isolates from medium altitude, and five lowland isolates. In morphological observations, there were striking differences in colony color and growth rate among isolates on several media and pathogenicity tests. Each isolate had different pathogenicity, although from the same location but other parts of the plant. Knowing the characteristic morphology and Fusarium sp. response of the three areas can be used as references in controlling.

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MYCELIAL GROWTH AND SPORULATION OF Fusarium spp. PATHOGENIC TO Ilex paraguariensis IN DIFFERENT CULTURE MEDIA AND UNDER EXPOSURE TO DIFFERENT LIGHT LEVELS.

Cited by 7 publications

References 3 publications

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Fusarium pallidoroseum: A potential entomopathogenic agent for the biological management of Aphis gossypii

Variability of Fusarium oxysporum f. sp. lycopersici from different altitudes in East Java, Indonesia

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