Mycelium-bound lipase from Aspergillus oryzae as efficient biocatalyst for cis-3-hexen-1-yl acetate synthesis in organic solvent

Kirdi, Rachida; Akacha, Najla Ben; Bejaoui, Hend; Messaoudi, Yosra; Romano, Diego; Molinari, Francesco; Gargouri, M.

doi:10.1016/j.bcab.2017.01.009

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…The culture was carried out at 20 °C for 4 days. The esterification reaction was carried out in n-hexane with 2% w/v of the biocatalyst and an equimolar 50 mM citronellol and acetic acid concentration at 40 °C for 8 h Means for initial reaction rate with different superscript letters denote a significant difference at p < 0.05 OM -BM medium with optimal concentration 0.7% of Tween 80 [16,27,47] due to the low water content in the reaction environment, which enhances enzyme denaturation [48], and the natural immobilization of the enzyme into the biomass exerting a protective effect [49]. Dry mycelium of Rhizopus oryzae was used for synthesis of different flavor esters (hexyl acetate and butyrate, geranyl acetate and butyrate).…”

Section: Discussionmentioning

confidence: 99%

“…In esterification of isoamyl alcohol with acetic acid by biomass-bound lipase from A. niger NCIM 1207, 100% conversion of the substrates was achieved in a reaction carried out in hexane at 30 °C for 96 h [51]. A high esterification yield (98%) of cis-3-hexen-1-ol and acetic acid using biomass-bound lipase from Aspergillus oryzae was obtained at 70 °C [47].…”

Section: Discussionmentioning

confidence: 99%

“…4). Kirdi et al [47] reported that A. oryzae biomass-bound lipase retained approx. 60% of activity after five 24-h reaction cycles.…”

Section: Discussionmentioning

confidence: 99%

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Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

et al. 2021

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A biomass-bound lipase from psychrophilic Chrysosporium pannorum A-1 is an efficient biocatalyst for direct esterification of β-citronellol and acetic acid in an organic solvent. The biomass is effectively produced by fungal submerged culture at 20 ℃, which results in lower energy consumption during the production of biocatalyst. Supplementation of the culture medium with calcium carbonate together with olive oil contributed to a significant increase in the active biomass of mycelium in one batch culture and increased the efficiency of the biocatalyst. Biomass-bound lipase showed high catalytic activity in a broad temperature range of 30–60 °C and stability up to 70 °C. A maximum molar conversion value of 98% was obtained at 30 °C in n-hexane using a 2:1 alcohol-to-acid molar ratio and 3% w/v of the biocatalyst within 24 h. The high equimolar concentration of the substrates (200 mM) did not have an adverse effect on mycelial biomass activity. Dry mycelium of C. pannorum is a promising biocatalyst for large-scale biosynthesis of citronellyl acetate, given its low-cost production, high activity at low temperatures, and reusability in a minimum of seven 24-h biocatalytic cycles.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

et al. 2021

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Solvent stable microbial lipases: current understanding and biotechnological applications

Priyanka¹,

Tan²,

Kinsella³

et al. 2018

Biotechnol Lett

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Objective: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment. Results: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases. Conclusion: Organic solvent stable lipases are, and will continue to be, versatile and adaptable biocatalytic workhorses commonly employed for industrial applications in the food, pharmaceutical and green manufacturing industries.

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Electrochemical mineralization of uric acid with boron-doped diamond electrode: Factor analysis and degradation mechanism

et al. 2019

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Mycelium-bound lipase from Aspergillus oryzae as efficient biocatalyst for cis-3-hexen-1-yl acetate synthesis in organic solvent

Cited by 5 publications

References 43 publications

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

Solvent stable microbial lipases: current understanding and biotechnological applications

Electrochemical mineralization of uric acid with boron-doped diamond electrode: Factor analysis and degradation mechanism

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