Mycobacteria of Runyon groups I, II and IV do not play an aetiological role in Crohn??s disease

Kreuzpaintner, G.; Kirschner, Philip; Wallner, A.; R, Kölble; Hesterberg, R.; Thomas, Lutz; Borchard, F

doi:10.1097/00042737-199512000-00009

Cited by 9 publications

(3 citation statements)

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“…There was statistically significant evidence of heterogeneity (chi‐square = 468.99, P < 0.001). The summary measure of risk difference from a random effects model was 0.20 (95% CI, 0.12–0.28) and remained almost identical after excluding the study by Kreuzpaintner et al,23 which did not use IS900 (0.20; 95% CI, 0.12–0.28). Similarly, exclusion of any single study did not significantly alter the summary measure.…”

Section: Resultsmentioning

confidence: 81%

“…The exclusion of the study with a different target sequence23 did not alter the results. A number of studies included immunosuppressed patients.…”

Section: Discussionmentioning

confidence: 92%

“…Forty‐two PCR studies used the IS900 target sequence, while 1 used 16s rRNA 23. Four hybridization assays used the insertion sequence IS900 as the target.…”

Section: Resultsmentioning

confidence: 99%

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Detection of Mycobacterium avium subspecies paratuberculosis from patients with Crohnʼs disease using nucleic acid-based techniques: A systematic review and meta-analysis

Abubakar

Myhill

Aliyu

et al. 2008

Inflammatory Bowel Diseases

180

144

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This study is a systematic review and meta-analysis of studies using nucleic acid-based techniques to detect Mycobacterium avium paratuberculosis (MAP) in patients with Crohn's disease (CD) compared with controls. Database searches were conducted and risk difference estimates were calculated using meta-analysis. Fifty-eight studies were reviewed, 47 of which were included in the analysis. The pooled estimate of risk difference from all studies was 0.23 (95% confidence interval [CI], 0.14-0.32) using a random effects model. Similarly, MAP was detected more frequently from patients with CD compared with those with ulcerative colitis (risk difference 0.19, 95% CI, 0.10-0.28). Year of study, assay type, and inclusion of children explained some but not all of the observed heterogeneity. The data confirms the observation that MAP is detected more frequently among CD patients compared with controls. However, the pathogenic role of this bacterium in the gut remains uncertain. Our analysis demonstrates that there is an association between MAP and CD, across many sites, by many investigators, and controlling for a number of factors; however, this association remains controversial and inconclusive. Future studies should determine whether there is a pathogenic role.

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Section: Resultsmentioning

confidence: 81%

“…The exclusion of the study with a different target sequence23 did not alter the results. A number of studies included immunosuppressed patients.…”

Section: Discussionmentioning

confidence: 92%

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Detection of Mycobacterium avium subspecies paratuberculosis from patients with Crohnʼs disease using nucleic acid-based techniques: A systematic review and meta-analysis

Abubakar

Myhill

Aliyu

et al. 2008

Inflammatory Bowel Diseases

180

144

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Detection of fastidious mycobacteria in human intestines by the polymerase chain reaction

Dumonceau

Gossum

Adler

et al. 1997

Eur. J. Clin. Microbiol. Infect. Dis.

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The aim of this study was to determine whether difficult-to-grow mycobacteria are present in human intestines. Intestinal tissue samples were subjected to both mycobacterial culture and a polymerase chain reaction (PCR) assay. After detection by PCR, species identity was determined by hybridizing the amplified 16S rRNA gene fragments with species-specific oligonucleotides. Intestinal biopsies from 63 patients with noninflammatory bowel diseases (n = 22), Crohn's disease (n = 31), or ulcerative colitis (n = 10) were analyzed. Culture and PCR revealed mycobacteria in four (6%) and 25 (40%) samples, respectively. Samples positive by PCR were negative with all probes specific to nine common cultivable species but were positive with Mycobacterium genavense-specific probe in 68% of cases. Mycobacterial isolates were identified as Mycobacterium gordonae and Mycobacterium chelonae. Findings were similar in Crohn's disease samples compared to non-Chron's disease samples. This study shows that difficult-to-grow mycobacteria can be detected by PCR in large and similar proportions of inflamed intestinal tissue from patients with inflammatory bowel disease and intestinal tissue that appears normal from patients with noninflammatory bowel disease.

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The Zoonotic Potential of Mycobacterium avium spp. paratuberculosis

Waddell

Rajić

Sargeant

et al. 2008

Can J Public Health

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Background:The zoonotic potential of Mycobacterium avium ssp. paratuberculosis (MAP) has been debated for almost a century because of similarities between Johne's Disease (JD) in cattle and Crohn's disease (CD) in humans. Our objective was to evaluate scientific literature investigating the potential association between these two diseases (MAP and CD) and the presence of MAP in retail milk or dairy products using a qualitative systematic review. Method:The search strategy included 19 bibliographic databases, 8 conference proceedings, reference lists of 15 articles and contacting 28 topic-related scientists. Two independent reviewers performed relevance screening, quality assessment and data extraction stages of the review.Results: Seventy-five articles were included. Among 60 case-control studies that investigated the association between MAP and CD, 37 were of acceptable quality. Twenty-three studies reported significant positive associations, 23 reported non-significant associations, and 14 did not detect MAP in any sample. Different laboratory tests, test protocols, types of samples and source populations were used in these studies resulting in large variability among studies. Seven studies investigated the association between CD and JD, two challenge trials reported contradictory results, one cross-sectional study did not support the association, and four descriptive studies suggested that isolated MAP is often closely related to cattle isolates. MAP detection in raw and pasteurized milk was reported in several studies. Conclusions:Evidence for the zoonotic potential of MAP is not strong, but should not be ignored. Interdisciplinary collaboration among medical, veterinary and other public health officials may contribute to a better understanding of the potential routes of human exposure to MAP.

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Mycobacteria of Runyon groups I, II and IV do not play an aetiological role in Crohn??s disease

Cited by 9 publications

References 0 publications

Detection of Mycobacterium avium subspecies paratuberculosis from patients with Crohnʼs disease using nucleic acid-based techniques: A systematic review and meta-analysis

Detection of Mycobacterium avium subspecies paratuberculosis from patients with Crohnʼs disease using nucleic acid-based techniques: A systematic review and meta-analysis

Detection of fastidious mycobacteria in human intestines by the polymerase chain reaction

The Zoonotic Potential of Mycobacterium avium spp. paratuberculosis

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