Mycobacterial Interspersed Repeat Unit–Variable Number Tandem Repeat Typing of Mycobacterium avium Strains Isolated from the Lymph Nodes of Free-Living Carnivorous Animals in Poland

Orłowska, Blanka; Majchrzak, Marta; Didkowska, Anna; Anusz, Krzysztof; Krajewska-Wędzina, Monika; Zabost, Anna; Brzezińska, Sywia; Kozińska, Monika; Augustynowicz-Kopeć, Ewa; Urbańska, Kaja; Welz, Mirosław; Parniewski, Paweł

doi:10.3390/pathogens12091184

Cited by 2 publications

(1 citation statement)

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“…Mycobacterial genotyping methods. Genomic DNA the bacterial culture was isolated based on a previously described protocol [8]. Strain genotyping was performed by spoligotyping (Ocimum Biosolutions, Hyderabad, India) using commercially available membranes according to the standard protocol (https://gentaur.es/wp-content/ uploads/2015/03/Spoligotyping-Manual.pdf ).…”

Section: Mgit Tbcidentification Test® the Bd Mgit Tbcmentioning

confidence: 99%

<i>Mycobacterium caprae</i> tuberculosis in a captive lion in Ukraine – Case Report

Didkowska,

Krajewska-Wędzina,

Miller

et al. 2024

Ann Agric Environ Med.

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Introduction and Objective.Because of the armed conflict in Ukraine, companion, farm and captive wild animals have been moved in a simplified procedure across the Polish-Ukrainian border. For that reason, in 2022, Poznań Zoo provided support for almost 200 wild animals before movement to facilities in other countries. The aim of the study is to identify the under-recognized risk of moving animals that may be infected with zoonotic pathogens, such as Mycobacterium caprae, between countries. Materials and Method. Sections of the heart, lungs, and mesentery of 4-year-old lioness from Ukraine were taken for histopathological and bacteriological examination. Results. Microbiological examinations confirmed the presence of Mycobacterium caprae, SB0418 spoligotype. Conclusion. TB is a zoonotic disease present globally. Movement of captive wild animals from regions with MTBC cases, or lack of MTBC surveillance, such as UA may pose a potential threat to public health.

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Section: Mgit Tbcidentification Test® the Bd Mgit Tbcmentioning

confidence: 99%

<i>Mycobacterium caprae</i> tuberculosis in a captive lion in Ukraine – Case Report

Didkowska,

Krajewska-Wędzina,

Miller

et al. 2024

Ann Agric Environ Med.

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Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification

Zhang,

Tang,

et al. 2024

Front. Public Health

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Non-tuberculous mycobacteria (NTM) infections pose a significant public health challenge worldwide, affecting individuals across a wide spectrum of immune statuses. Recent epidemiological studies indicate rising incidence rates in both immunocompromised and immunocompetent populations, underscoring the need for enhanced diagnostic and therapeutic approaches. NTM infections often present with symptoms similar to those of tuberculosis, yet with less specificity, increasing the risk of misdiagnosis and potentially adverse outcomes for patients. Consequently, rapid and accurate identification of the pathogen is crucial for precise diagnosis and treatment. Traditional detection methods, notably microbiological culture, are hampered by lengthy incubation periods and a limited capacity to differentiate closely related NTM subtypes, thereby delaying diagnosis and the initiation of targeted therapies. Emerging diagnostic technologies offer new possibilities for the swift detection and accurate identification of NTM infections, playing a critical role in early diagnosis and providing more accurate and comprehensive information. This review delineates the current molecular methodologies for NTM species and subspecies identification. We critically assess the limitations and challenges inherent in these technologies for diagnosing NTM and explore potential future directions for their advancement. It aims to provide valuable insights into advancing the application of molecular diagnostic techniques in NTM infection identification.

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Mycobacterial Interspersed Repeat Unit–Variable Number Tandem Repeat Typing of Mycobacterium avium Strains Isolated from the Lymph Nodes of Free-Living Carnivorous Animals in Poland

Cited by 2 publications

References 64 publications

<i>Mycobacterium caprae</i> tuberculosis in a captive lion in Ukraine – Case Report

<i>Mycobacterium caprae</i> tuberculosis in a captive lion in Ukraine – Case Report

Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification

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