Mycobacterial Methyltransferases: Significance in Pathogenesis and Virulence

Grover, Sonam; Gangwar, Rishabh; Jamal, Salma; Ali, Sabeeha; Nisaa, Khairun; Ehtesham, Nasreen Z.; Hasnain, Seyed E.

doi:10.1007/978-981-32-9413-4_7

Cited by 7 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The methylation is involved in virulence and influences the mechanism of M.tb. pathogenesis (Grover et al, 2019). We checked the mmaA4 expression defects impacting upon the enzyme activity and observed SAM-MTs activity was inhibited in log phase culture of mutant BCG compare to wild type (Fig.…”

Section: Discussionmentioning

confidence: 99%

Protein kinase J regulates Rv0642c expression and sensitivity to rifampicin of mycobacteria

Singh

Tiwari

et al. 2021

Preprint

View full text Add to dashboard Cite

Mycobacterium tuberculosis (M.tb) persists for long-duration inside the human host in both active and latent form by modulating the immune response. The mechanisms employed by M.tb to survive inside the host and evade the host immune system need to be explored in greater depth for the rational design of novel treatment strategies. The phosphorylation and methylation of biomolecules need to be addressed in mycobacteria because it has an important role in infection establishment and persistence. In the present study, we elaxborate on the role of PknJ in the slow growth of BCG and its association with mmaA4 protein during extracellular and intracellular growth. The pknJ knock-out (KO) BCG has been used to decode the functional significance in mycobacteria. The mmaA4 expression and methyltransferase activity is decreased in knocked-out BCG strain (pknJ-/-) during extracellular growth, while mmaA4 expression and methyltransferase activity is increased during intracellular growth of mycobacteria. The knocked-out BCG strain is highly sensitive to the rifampicin antibiotics during extracellular growth in compared to control. A significant association of pknJ and mmaA4 was found in our studies during the growth and intracellular persistence of mycobacteria.

show abstract

Section: Discussionmentioning

confidence: 99%