Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal and environmental samples

Abstract

ABSTRACT:Mycobacterium arupense is a non-tuberculous, potentially pathogenic species rarely isolated from humans. The aim of the study was to ascertain the spectrum of non-tuberculous mycobacteria within 271 sequenced mycobacterial isolates not belonging to M. tuberculosis and M. avium complexes. Isolates were collected between 2004 and 2009 in the Czech Republic and were examined within the framework of ecological studies carried out in animal populations infected with mycobacteria. A total of thirty-three mycobacterial species were identified. This report describes the isolation of M. arupense from the sputum of three human patients and seven different animal and environmental samples collected in the last six years in the Czech Republic: one isolate from leftover refrigerated organic dog food, two isolates from urine and clay collected from an okapi (Okapia johnstoni) and antelope bongo (Tragelaphus eurycerus) enclosure in a zoological garden, one isolate from the soil in an eagle's nest (Haliaeetus albicilla) band two isolates from two common vole (Microtus arvalis) livers from one cattle farm. All isolates were identified by biochemical tests, morphology and 16S rDNA sequencing. Also, retrospective screening for M. arupense occurrence within the collected isolates is presented.

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

“…It has been isolated from surface water, municipal water supplies, and biofilms from DWDSs (September et al 2004). Mycobacterium arupense is a novel and potentially pathogenic Mycobacterium species, which has been isolated from human clinical samples (Neonakis et al 2010), small terrestrial mammals, and surface water (Slany et al 2010). It was reported that M. arupense causes tenosynovitis in a patient with diabetes mellitus (Tsai et al 2008).…”

Mycobacterium minnesotense sp. nov., a photochromogenic bacterium isolated from sphagnum peat bogs

“…Unlike Mycobacterium tuberculosis, non-tuberculous mycobacteria (NTM) are not obligate pathogens and can be found in a number of different environmental habitats (Falkinham, 2009(Falkinham, , 2010Slany et al, 2010). In recent years, NTM have become an increasing threat to humans as opportunistic infectious agents (Cook, 2010;Cassidy et al, 2009).…”

Molecular Detection of Nontuberculous Mycobacteria: Advantages and Limits of a Broad-Range Sequencing Approach

“…Precise identification of bacterial pathogens using sequence analysis often fail due to very similar or identical sequence homologies within the bacterial genus (e.g. M. peregrinum vs. M. septicum ) [Slany et al, 2010b]. Sequence analysis is unable to distinguish individual members of M. tuberculosis complex, as they are characterized by 99.9% conserved nucleotide identity for the 16S rRNA gene [Brosch et al, 2002].…”

“…Briefly, morphologically distinct colonies from primary culture are subcultured and then 16S rRNA sequencing is carried out [Slany et al, 2010a]. The advantage of using ribosomal sequences for genotypic identification of mycobacteria lies in the possibility of recognizing previously undescribed species [Slany et al 2010b;Springer et al, 1996]. It should be noted that detection and identification of NTM by this approach is limited by successful cultivation.…”