Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy.

Wilson, Rebecca W.; Steingrube, Vincent A.; Böttger, Erik C.; Springer, Burkhard; Brown‐Elliott, Barbara A.; Vincent, Véronique; Jost, Kenneth C.; Zhang, Yansheng; García, María Jesús García; Chiu, S. H.; Onyi, Grace O.; Rossmoore, H.W.; Nash, Donald R.; Wallace, Richard J.

doi:10.1099/00207713-51-5-1751

Cited by 200 publications

(187 citation statements)

References 52 publications

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“…With the data from the five combined genes, M. immunogenum appears to be a hybrid between M. abscessus and M. chelonae, with a 55 % bootstrap value. This phenomenon was previously described using PRA band patterns from a 439-bp segment of the hsp65 gene digested with BstEII and HaeIII (Wilson et al, 2001). Further studies are warranted to assess better the taxonomic relationships of this emerging taxon.…”

Section: Evidence From Insertions and Deletions (Molecular Signatures)mentioning

confidence: 99%

“…They formed a defined cluster in the 16S rRNA and hsp65 gene phylogenies (Tortoli, 2003;Wilson et al, 2001). The recently described M. immunogenum was added to this group according to its phenotypic characteristics, its unique hsp65 sequence pattern and its 15-18 % DNA-DNA relatedness to other species of this group (Wilson et al, 2001).…”

Section: Evidence From Insertions and Deletions (Molecular Signatures)mentioning

confidence: 99%

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Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing

Adékambi

Drancourt

2004

International Journal of Systematic and Evolutionary Microbiology

276

191

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The current classification of non-pigmented and late-pigmenting rapidly growing mycobacteria (RGM) capable of producing disease in humans and animals consists primarily of three groups, the Mycobacterium fortuitum group, the Mycobacterium chelonae-abscessus group and the Mycobacterium smegmatis group. Since 1995, eight emerging species have been tentatively assigned to these groups on the basis of their phenotypic characters and 16S rRNA gene sequence, resulting in confusing taxonomy. In order to assess further taxonomic relationships among RGM, complete sequences of the 16S rRNA gene (1483-1489 bp), rpoB (3486-3495 bp) and recA (1041-1056 bp) and partial sequences of hsp65 (420 bp) and sodA (441 bp) were determined in 19 species of RGM. Phylogenetic trees based upon each gene sequence, those based on the combined dataset of the five gene sequences and one based on the combined dataset of the rpoB and recA gene sequences were then compared using the neighbour-joining, maximum-parsimony and maximum-likelihood methods after using the incongruence length difference test. Combined datasets of the five gene sequences comprising nearly 7000 bp and of the rpoB+recA gene sequences comprising nearly 4600 bp distinguished six phylogenetic groups, the M. chelonae-abscessus group, the Mycobacterium mucogenicum group, the M. fortuitum group, the Mycobacterium mageritense group, the Mycobacterium wolinskyi group and the M. smegmatis group, respectively comprising four, three, eight, one, one and two species. The two protein-encoding genes rpoB and recA improved meaningfully the bootstrap values at the nodes of the different groups. The species M. mucogenicum, M. mageritense and M. wolinskyi formed new groups separated from the M. chelonae-abscessus, M. fortuitum and M. smegmatis groups, respectively. The M. mucogenicum group was well delineated, in contrast to the M. mageritense and M. wolinskyi groups. For phylogenetic organizations derived from the hsp65 and sodA gene sequences, the bootstrap values at the nodes of a few clusters were <70 %. In contrast, phylogenetic organizations obtained from the 16S rRNA, rpoB and recA genes were globally similar to that inferred from combined datasets, indicating that the rpoB and recA genes appeared to be useful tools in addition to the 16S rRNA gene for the investigation of evolutionary relationships among RGM species. Moreover, rpoB gene sequence analysis yielded bootstrap values higher than those observed with recA and 16S rRNA genes. Also, molecular signatures in the rpoB and 16S rRNA genes of the M. mucogenicum group showed that it was a sister group of the M. chelonae-abscessus group. In this group, M. mucogenicum ATCC 49650 T was clearly distinguished from M. mucogenicum ATCC 49649 with regard to analysis of the five gene sequences. This was in agreement with phenotypic and biochemical characteristics and suggested that these strains are representatives of two closely related, albeit distinct species.Abbreviations: ILD, incongruence length difference; K2P, Kimura's two-parameter...

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Section: Evidence From Insertions and Deletions (Molecular Signatures)mentioning

confidence: 99%

Section: Evidence From Insertions and Deletions (Molecular Signatures)mentioning

confidence: 99%

Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing

Adékambi

Drancourt

2004

International Journal of Systematic and Evolutionary Microbiology

276

191

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“…chelonae group. 19 Samples from TRW (and other machining environments), previously identified as M. chelonae, have been re-examined and found to be M. immunogenum.…”

Section: Mwf Aerosol and Respiratory Illnessmentioning

confidence: 99%

Health hazard evaluation report: HETA #2001-0303-2893, TRW Automotive, Mt. Vernon, Ohio.

2002

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“…Consequently, once mycobacteria have been detected in manufacturing operations using MWFs, unscheduled discharges of MWFs to wastewater treatment may be required, hence impacting wastewater treatment downstream and increasing downtime in metalworking processes. Aggressive formaldehyde condensate biocide-based control of microbial growth resulted in the change in bacterial flora favoring proliferation of a nontuberculous Mycobacterium and 95% of isolates, 102 of 107 isolates from 10 industrial sites, were identified to be Mycobacterium immunogenum (Wilson et al, 2001;Wallace et al, 2002). Due to these issues, M. immunogenum has emerged as a significant health hazard agent (Shelton et al, 1999;Wilson et al, 2001) in metalworking processes, and their accurate detection and quantification have become a necessity (Passman and Rossmoore, 2002).…”

Section: Introductionmentioning

confidence: 99%

Nano-Immunodetection and Quantification of Mycobacteria in Metalworking Fluids

Chang

Adriaens

2007

Environmental Engineering Science

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The accurate detection and enumeration of mycobacteria in metalworking fluids (MWFs) is imperative from an environmental protection and occupational health perspective. We report here on a comparison of the labeling efficiency of nano-immunomagnetic particles (NIMP) and free antibody (FAb) to detect mycobacteria in semisynthetic MWF by using both traditional visualization analysis and cluster analysisaided visualization analysis (CAAVA). The NIMP labeling method involved coating nanometer-scale magnetic particles with Protein A, and oriented conjugation of polyclonal antibodies specific to Mycobacterium spp. The FAb labeling method is modified from the traditional immunofluorescence (IF) method for more efficient detection of mycobacteria in a model MWF. The labeling efficiency of NIMP and FAb were 7.2 Ϯ 4.6 and 16.3 Ϯ 5.5%, and the specificity 85.0 Ϯ 6.1 and 88.1 Ϯ 10.5%, respectively, based on traditional visualization analysis. Based on CAAVA analysis, the labeling efficiency of NIMP and FAb increased to 12.4 Ϯ 1.6 and 20.5 Ϯ 3.9%, and the specificity to 97.8 Ϯ 3.2 and 98.5 Ϯ 2.5%, respectively. A linear relationship of FCM counts and seeded concentrations was observed over four orders of magnitude (R 2 Ͼ 0.99) in pure and ternary cultures. The results strongly support the applicability of either FAb or NIMP coupled with CAAVA and flow cytometry for rapid detection and enumeration of mycobacteria in complex matrices.

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Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy.

Cited by 200 publications

References 52 publications

Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing

Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing

Health hazard evaluation report: HETA #2001-0303-2893, TRW Automotive, Mt. Vernon, Ohio.

Nano-Immunodetection and Quantification of Mycobacteria in Metalworking Fluids

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