Mycobacterium massiliense BRA100 strain recovered from postsurgical infections: resistance to high concentrations of glutaraldehyde and alternative solutions for high level disinfection

Abstract

Purpose:To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD). Methods: Reference mycobacteria and clinical M. massiliense strains were included in this study. Active cultures were submitted to susceptibility qualitative tests with GTA dilutions (ranging from 1.5% to 8%), and commercial orthophthaldehyde (OPA) and peracetic acid (PA) -based solutions, during the period of exposure as recommended by National Agency of Sanitary Surveillance for HLD. Results: All reference and M. massiliense non-BRA100 strains, recovered from sputum, were susceptible to any GTA concentration, OPA and PA solutions. M. massiliense BRA100 strains presented MIC of 8% GTA and were susceptible to OPA and PA. Conclusion: M. massiliense BRA100 strain is resistant to high GTA concentrations (up to 7%), which proves that this product is non-effective against specific rapidly growing mycobacteria and should be substituted by OPA or PA -based solutions for HLD. Key words: Mycobacterium Infections. Videolaparoscopy. Disinfection. Glutaraldehyde. RESUMOObjetivo: Avaliar a concentração mínima inibitória (CMI) de GTA frente a M. massiliense e a susceptibilidade a produtos alternativos para desinfecção de alto nível (DAN). Métodos: Cepas de M. massiliense de origem clínica e de referência foram incluídas no estudo. As culturas ativadas foram submetidas a testes qualitativos com diluições de GTA (de 1,5% a 8%) e com soluções comerciais de ortoftaldeído (OPA) ou ácido peracético (PA), utilizando os tempos de exposição recomendados pela Agência Nacional de Vigilância Sanitária para DAN. Resultados: Todas as cepas de referência e M. massiliense não-BRA100, obtida de escarro, foram susceptíveis às concentrações de GTA, e soluções de OPA e PA. As cepas de M. massiliense BRA100 apresentaram CMI de 8% para GTA e foram susceptíveis a OPA e PA. Conclusão: M. massiliense BRA100 é resistente a altas concentrações de GTA (até 7%), o que demonstra que esse composto não é eficaz, e deve ser substituído por OPA ou PA nos processos de DAN.

Stability of antimicrobial activity of peracetic acid solutions used in the final disinfection process

“…9,10 However, little is known about the stability of the antimicrobial action of this product when in contact with instruments contaminated with microorganisms and organic material such as blood and saliva. These contaminated instruments must undergo a final disinfection prior to washing, which is known as "purging" in the hospital environment, to eliminate many or all pathogenic microorganisms.…”

Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates

“…Both patients had confirmed pulmonary disease and lived in RJ, where more than 1,000 possible surgical-site infection cases were reported to ANVISA (12), but these isolates harbor distinct biological properties, such as clarithromycin resistance and susceptibility to glutaraldehyde at Ͻ1.5%, while the epidemic strain is susceptible to clarithromycin and tolerant of glutaraldehyde. Therefore, they may be considered a different strain or a biological variant of the epidemic strain (12,20).…”

Port Site Tuberculosis after Laparoscopic Cholecystectomy: A Study of 20 Cases

“…Most of the studies recommend washing with autoclaved water and or sterile normal saline and immediate drying of the instruments. [13] The most commonly used agent for sterilization of laparoscopic instruments is 2% glutaraldehyde. Instruments (both ports with trocars and hand instruments) need to be immersed in the said solution for at least 10 hours for proper sterilization and a minimum of twenty minutes for disinfection.…”