Mycobacterium syngnathidarum sp. nov., a rapidly growing mycobacterium identified in syngnathid fish

Fogelson, Susan B.; Camus, Alvin C.; Lorenz, W. Walter; Phillips, Ashley; Bartlett, Paula J.; Sánchez, Susan

doi:10.1099/ijsem.0.002978

Cited by 15 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…chelonae-abscessus complex, as well as two divergent outliers (pipefish and seahorse1) cultured from syngnathid fish. Additional targeted gene sequencing, dDDH, and PRA analysis ( S2 Table and S7 Fig ) established the two outliers as a novel species, Mycobacterium syngnathidarum [ 60 ]. Core genome analysis of the remaining 41 whole mycobacterial genomes separated the human “ M .…”

Section: Discussionmentioning

confidence: 99%

Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns

et al. 2019

Self Cite

View full text Add to dashboard Cite

Mycobacterium chelonae is a member of the Mycobacterium chelonae-abscessus complex and a cause of opportunistic disease in fish, reptiles, birds, and mammals including humans. Isolates in the complex are often difficult to identify and have differing antimicrobial susceptibilities. Thirty-one previously identified rapidly-growing, non-tuberculous Mycobacterium sp. isolates cultured from biofilms, fish, reptiles, mammals, including humans, and three ATCC reference strains were evaluated with nine M. chelonae-abscessus complex whole genome sequences from GenBank by phylogenomic analysis, targeted gene comparisons, and in-vitro antimicrobial susceptibility patterns to assess strain variation among isolates from different sources. Results revealed minimal genetic variation among the M. chelonae strains. However, the core genomic alignment and SNP pattern of the complete 16S rRNA sequence clearly separated the turtle type strain ATCC 35752T from the clinical isolates and human reference strain “M. chelonae chemovar niacinogenes” ATCC 19237, providing evidence of two distinct subspecies. Concatenation of the partial rpoB (752 bp) and complete hsp65 (1,626 bp) sequence produced the same species/subspecies delineations as the core phylogeny. Partial rpoB and hsp65 sequences identified all the clinical isolates to the appropriate species level when respective cut-offs of 98% and 98.4% identity to the M. chelonae type strain ATCC 35752T were employed. The human strain, ATCC19237, was the most representative strain for the evaluated human, veterinary, and environmental strains. Additionally, two isolates were identified as Mycobacterium saopaulense, its first identification in a non-fish or non-human host.

show abstract

Section: Discussionmentioning

confidence: 99%

Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…M. syngnathidarum, which was the closest MAG relative in the no residual unfiltered system, on the other hand, is a recently described fish pathogen . A handful of previous studies have examined differences in mycobacterial species abundances in no residual versus chloraminated versus chlorinated water. ,,, One recent study noted differences in abundances of Mycobacterium intracellualre, M.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Profiling of Pathogens in Simulated Reclaimed Water Distribution Systems Operated at Elevated Temperature Reveals Distinct Response of Mycobacteria to Filtration and Disinfection Conditions

et al. 2023

View full text Add to dashboard Cite

Distribution of reclaimed water presents distinct challenges for pathogen control relative to the distribution of conventional potable water. The elevated temperatures common in arid regions can exacerbate such challenges by accelerating disinfectant decay and increasing microbial growth rates. Here, we carried out a controlled, continuous-flow, head-to-head study of six simulated reclaimed water distribution systems (RWDSs) in order to assess whether treatments identified to be effective for microbiological control at a lower temperature (22 °C) were also effective at 30 °C. Shotgun metagenomic sequencing was carried out to comprehensively profile fecal pathogens (FPs) (e.g., Escherichia and Klebsiella spp.), non-FPs (non-FPs) (e.g., Legionella and Mycobacterium spp.), and antibiotic-resistance genes (ARGs). Consistent with prior studies, generally, all FP, non-FP, and ARG markers were lowest when the influent water was biologically active carbon (BAC) filtered and disinfected. However, in contrast to observations at the lower temperature, where BAC filtration combined with chlorine disinfection effectively controlled Mycobacterium spp., they were markedly elevated in BAC-filtered and chlorine-or chloramine-disinfected conditions. This study highlights the need for further research to improve the understanding of conditions that select for different mycobacterial species and identify strategies to effectively control them in RWDSs under elevated temperature conditions.

show abstract

“…Multinucleated giant cells, epithelioid macrophages, lymphocytes and surrounding connective tissue were not observed as in other teleosts [ 27 ]. For this reason, this microscopic finding has been previously named atypical granulomas [ 3 , 20 ]. Currently, it is unclear as to whether these atypical lesions are the result of an ineffective cell-mediated immune response, the virulence of the pathogen or a combination of both.…”

Section: Discussionmentioning

confidence: 99%

“…Captive breeding and re-introduction programs have been applied for the last few decades in an attempt to reverse this trend [ 18 ]. However, the captive breeding of many species of syngnathids remains a challenge due to their susceptibility to diseases and management issues such as stress control, water quality management and high population densities [ 19 , 20 ]. As very little is known about the development of diseases in these animals in captivity, the objectives of this work were to: (i) provide a detailed description of the clinical and pathological findings associated with mycobacterial infections; (ii) identify the causative etiological agents.…”

Section: Introductionmentioning

confidence: 99%

Clinical and Pathological Findings Associated with Mycobacteriosis in Captive Syngnathids

Montero

Rojo-Solís²,

Castro³

et al. 2022

Animals

View full text Add to dashboard Cite

Mycobacteriosis is an important disease that affects captive and wild aquatic fish. Syngnathids are susceptible to infection by non-tuberculous mycobacteria. The aim of this study was to describe clinical signs, and macroscopic and histological lesions in 25 syngnathids and the molecular characterization of the causative mycobacteria. Clinical presentation ranged from sudden death to non-specific signs, including anorexia, poor body condition, weight loss and marked dyspnea with increased respiratory effort and rate. Gross lesions were mostly ulcers on the tail and small white nodules in the liver, coelomic cavity and inside the eye. The most affected organs were gills, liver, intestine and coelomic mesentery. Microscopic lesions consisted of areas of multifocal to diffuse granulomatous inflammation and bacterial emboli with numerous intralesional acid-fast bacilli. Epithelioid cells, multinucleated giant cells, lymphocytes and fibrous connective tissue, which are commonly observed in granulomatous inflammation, were not observed here. In the real-time PCR, M. fortuitum, M. chelonae and M. marinum common primers, Mycobacterium spp. were detected in 4, 7 and 14 individuals, respectively. In addition, this is the first description of mycobacteriosis found in Syngnathus acus.

show abstract

Mycobacterium syngnathidarum sp. nov., a rapidly growing mycobacterium identified in syngnathid fish

Cited by 15 publications

References 26 publications

Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns

Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns

Metagenomic Profiling of Pathogens in Simulated Reclaimed Water Distribution Systems Operated at Elevated Temperature Reveals Distinct Response of Mycobacteria to Filtration and Disinfection Conditions

Clinical and Pathological Findings Associated with Mycobacteriosis in Captive Syngnathids

Contact Info

Product

Resources

About