Mycobacterium tuberculosis Culture Supernatant Induces Cancer Cell Apoptosis and Cell Cycle Arrest

Abstract

Human lung cancer remains one of the deadliest diseases worldwide. New approaches are needed for improved lung cancer treatment. In this study, we found that M. tuberculosis culture supernatant (TB-SN) could inhibit human lung cancer cell proliferation through a caspase-dependent apoptosis pathway and induce cell cycle arrest in G1 phase. The active components responsible for the growth inhibitory activities were attributed to some proteins or protein complex with molecular weight more than 100 kD. These findings are significant and may provide new insight into a possible antagonism between M. tuberculosis and lung cancer and also have implications for development of an alternative approach for lung cancer treatment.

Mechanistic modeling of the effects of myoferlin on tumor cell invasion

“…Discussion MYOF belongs to the evolutionarily ancient ferlin family, which has been associated with a variety of processes important to cell migration and invasion, such as cell motility, growth factor receptor stability, endocytosis, and membrane repair (7-10, 11, 13). Although MYOF and the ferlin family have not previously been studied for their role in cancer, several reports suggest an association between MYOF overexpression and metastatic cancer (14,15,(17)(18)(19)(20)(21)(22)(23)(24)(25)(26). In this work we used a combination of mathematical modeling and experimental validation to uncover MYOF as a cancer protein, which we propose may be involved in regulating cancer cell invasion.…”

“…Although information on MYOF is currently limited, it has been shown to be upregulated in breast cancer biopsies (14) and expressed in breast cancer cell lines (15). Immunohistochemical evidence available from the Human Protein Atlas (16) suggests that MYOF is strongly expressed in several cancer types including colorectal, breast, ovarian, cervical, endometrial, thyroid, stomach, pancreatic, and liver cancer (14,15,(17)(18)(19)(20)(21)(22)(23)(24)(25)(26).To explore the function of MYOF in cancer, a stable line of MYOF-deficient malignant breast carcinoma cells (MDA-MB-231) was generated using lentivirus-based delivery of shRNA constructs targeting human MYOF mRNA (Sigma). A stable, lentiviral control cell line was generated in tandem using lentiviral particles carrying a nonhuman gene targeting shRNA (Sigma).…”