Mycobacterium tusciae sp. nov.

Tortoli, Enrico; Kroppenstedt, Reiner M.; Bartoloni, Alessandro; Caroli, G; Jan, Isabelle; Pawłowski, Jan; Emler, Stefan

doi:10.1099/00207713-49-4-1839

Cited by 57 publications

(29 citation statements)

References 23 publications

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“…It is shared, among others, by MAC, M. xenopi, and M. scrofulaceum ( Table 2). The problems associated with distinguishing M. tusciae from M. scrofulaceum are present also in GLC analysis (Table 3), while the HPLC pattern, characterized by an early major cluster of peaks, differs from those of any species reported so far (149).…”

Section: Tusciaementioning

confidence: 93%

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

2003

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The advancement of genetic techniques has greatly boosted taxonomic studies in recent years. Within the genus Mycobacterium, 42 new species have been detected since 1990, most of which were grown from clinical samples. Along with species for which relatively large numbers of strains have been reported, some of the new species of mycobacteria have been detected rarely or even only once. From the phenotypic point of view, among the new taxa, chromogens exceed nonchromogens while the numbers of slowly and rapidly growing species are equivalent. Whereas conventional identification tests were usually inconclusive, an important role was played by lipid analyses and in particular by high-performance liquid chromatography. Genotypic investigations based on sequencing of 16S rRNA gene have certainly made the most important contribution. The investigation of genetic relatedness led to the redistribution of the species previously included in the classically known categories of slow and rapid growers into new groupings. Within slow growers, the intermediate branch related to Mycobacterium simiae and the cluster of organisms related to Mycobacterium terrae have been differentiated; among rapid growers, the group of thermotolerant mycobacteria has emerged. The majority of species are resistant to isoniazid and, to a lesser extent, to rifampin. Many of the new species of mycobacteria are potentially pathogenic, and there are numerous reports of their involvement in diseases. Apart from disseminated and localized diseases in immunocompromised patients, the most frequent infections in immunocompetent people involve the lungs, skin, and, in children, cervical lymph nodes. The awareness of such new mycobacteria, far from being a merely speculative exercise, is therefore important for clinicians and microbiologists

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Section: Tusciaementioning

confidence: 93%

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

2003

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“…The isolates included representatives from the four major physiological groups: the MTC, the MAC, the RGM, and the slow-growing mycobacteria. Two newer species, M. tusciae and M. doricum, were also included to assess the technology, since they represented less-wellknown members of this genus (37,39).…”

Section: Discussionmentioning

confidence: 99%

“…With the rise of opportunistic pathogens and with several new species being named annually (5,28,37,39,40), the need to have a rapid and reproducible identification method for a wider range of Mycobacterium species is evident. Recent developments in mass spectrometry (MS) have made it possible to use whole-cell matrix-assisted laser desorption ionizationtime-of-flight MS (MALDI-TOF MS) or MALDI to aid in the identification of microorganisms.…”

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confidence: 99%

Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry

Pignone¹,

Greth²,

Cooper

et al. 2006

J Clin Microbiol

149

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Classical methods for identification of Mycobacterium species rely on morphology and biochemical profiles. Speciation of a Mycobacterium isolate using these standard methods is a lengthy process based on subjective data interpretation. In this study, Mycobacterium species were characterized by utilizing matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). This technology is designed to provide a characteristic mass spectral fingerprint based on desorbed ions from the cell surface. Thirty-seven strains were analyzed; these represented thirteen species and five subspecies that included the Mycobacterium tuberculosis complex and the M. avium-M. intracellulare complex, as well as rapid-and slow-growing mycobacteria. All 37 strains were analyzed in triplicate, and a database was generated. This method produced species-specific patterns for all but 1 of the 37 isolates and provided reliable differentiation at the strain level. The data suggest that whole-cell MALDI-TOF MS has potential as a rapid and reproducible method for the identification and characterization of Mycobacterium species.

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“…Mycobacteria belonging, from the phenotypic point of view, to slow growers, but genetically clustering with rapid growers, have been already reported elsewhere (12).…”

Section: Figmentioning

confidence: 99%

Burden of Unidentifiable Mycobacteria in a Reference Laboratory

et al. 2001

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Modern identification techniques at the genomic level have greatly improved the taxonomic knowledge of mycobacteria. In adjunct to nucleic acid sequences, mycobacterial identification has been endorsed by investigation of the lipidic patterns of unique mycolic acids in such organisms. In the present investigation, the routine use of high-performance liquid chromatography (HPLC) of mycolic acids, followed by the sequencing of the 16S rRNA, allowed us to select 72 mycobacterial strains, out of 1,035 screened, that do not belong to any of the officially recognized mycobacterial species. Most strains (i.e., 47) were isolated from humans, 13 were from the environment, 3 were from animals, and 9 were from unknown sources. The majority of human isolates were grown from the respiratory tract and were therefore most likely not clinically significant. Some, however, were isolated from sterile sites (blood, pleural biopsy, central venous catheter, or pus). Many isolates, including several clusters of two or more strains, mostly slow growers and scotochromogenic, presented unique genetic and lipidic features. We hope the data reported here, including the results of major conventional identification tests, the HPLC profiles of strains isolated several times, and the whole sequences of the 16S rRNA hypervariable regions of all 72 mycobacteria, may encourage reporting of new cases. The taxonomy of the genus Mycobacterium is, in our opinion, still far from being fully elucidated, and the reporting of unusual strains provides the best background for the recognition of new species. Our report also shows the usefulness of the integration of novel technology to routine diagnosis, especially in cases involving slow-growing microorganisms such as mycobacteria.

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Mycobacterium tusciae sp. nov.

Cited by 57 publications

References 23 publications

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry

Burden of Unidentifiable Mycobacteria in a Reference Laboratory

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