2008
DOI: 10.21608/bfsa.2008.64340
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Mycoflora, Mycotoxins, Bacteriological Analysis and Molecular Assay of Some Bacterial Species From Coffee Beans in Saudi Arabia

Abstract: ‫ﻤ‬ ٣١. ٤٣ ١٦ ٢٤ ١٨ ٢٥. while Staphylococcus appeared in 29 samples (55x103 cfu/g). All samples were free from Salmonella. PCR assay for detection of some bacterial species revealed that all samples were negative for Yersinia enterocolitica, Campylobacter and Listeria monocytogenes, while the results of B. cereus and Salmonella were similar to the results obtained using cultural method.

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Cited by 4 publications
(3 citation statements)
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“…However, the range of microbial load in the present study was broader than previous studies in Yemeni and Saudi Arabia green coffee (0.6 Â 104 to 5.5 Â 104 CFU) and (19 Â 10 to 18 Â 104 CFU), respectively. 32,33 The reasons for the difference in counts could be due to only dried beans were involved in previous studies while in this study in addition to dried beans, coffee fruits, fermented beans, and washed beans were included.…”
Section: Discussionmentioning
confidence: 99%
“…However, the range of microbial load in the present study was broader than previous studies in Yemeni and Saudi Arabia green coffee (0.6 Â 104 to 5.5 Â 104 CFU) and (19 Â 10 to 18 Â 104 CFU), respectively. 32,33 The reasons for the difference in counts could be due to only dried beans were involved in previous studies while in this study in addition to dried beans, coffee fruits, fermented beans, and washed beans were included.…”
Section: Discussionmentioning
confidence: 99%
“…Another study in Egypt investigated resistance in ten Salmonella strains isolated from poultry meat against amoxicillin-clavulanic acid (90%), trimethoprim-sulfamethoxazole (80%) and streptomycin, ampicillin-sulbactam, nalidixic acid, and gentamycin at 70% each [68]. In Morocco [58], 104 S. Enterica isolates from food origin were found resistant to tetracycline (21%), ampicillin (13%), amoxicillinclavulanic acid (9%), streptomycin (7%), chloramphenicol (4%) and nalidixic acid (3.8%). Because NTS is associated with increased morbidity and mortality, the determination of antimicrobial resistance profiles should be regarded as a vital part of NTS surveillance in food safety and public health laboratories in Arab countries.…”
Section: Non-typhoidal Salmonella In the Food Chain In The Arab Worldmentioning
confidence: 99%
“…Then, the volume was adjusted to 2.00 mL with a mixture of 0.0025 mol mL −1 HTAB and ammonia buffer (0.020 mol mL −1 , pH = 9.3). For test samples, the interferences considered were ZEN and STE because these mycotoxins can be found together with OTA in coffee and tea samples [31][32][33][34]. Accordingly, test samples were prepared in a similar way to validation solutions but with the addition of ZEN and STE solutions before evaporating the solvent and reconstituting the solution with a 2.00 mL mixture of HTAB and ammonia buffer.…”
Section: Calibration Validation and Test Samplesmentioning
confidence: 99%