Background: CSOM is a chronic inflammation of middle ear and mastoid cavity with recurrent ear discharge lasting for more than 6 months. Otomycosis is increasing in recent years because of the indiscriminate use of broad spectrum antibiotics, corticosteroids and an increase in the number of immune deficiency disorders, swimming, use of oils and ear drops. Aims and objectives: The study aimed to find out the fungal agents responsible for causing CSOM among the patients who attended Out Patient Department of ENT in collaboration with Department of Microbiology, Dr. P.D.M.M. College and Hospital, Amravati. Material and Methods: A Total of 130 patients who were clinically diagnosed with CSOM of all age groups and both sexes were selected for study from April 2018 to March 2019. Ear discharge was collected under aseptic precautions using sterile cotton swabs and direct microscopic examination and fungal culture was done. Slide culture done for differentiation of morphology of different species. Results: A total of 130 patients, Fungus isolated from 75 cases. Of which 49 were males and 26 were females. Main fungal isolates were Aspergillus species 51 (68 %), followed by Candida albicans 18 (24 %), Non albicans Candida 4 (5. 33 %), Penicillium 2 (2.66 %). Conclusion: CSOM with Otomycosis produce serious complications and treatment failure. Hence in cases of persistent otorrhoea, aural fullness, otalgia or any symptoms which signifies chronic middle ear infection, then fungal infection should be suspected. If the discharging ear is not responding with antibiotics aural toilet and fungal culture and sensitivity should be done in refractory cases before starting antibiotic therapy.