Mycoparasitism capability and growth inhibition activity of Clonostachys rosea isolates against fungal pathogens of grapevine trunk diseases suggest potential for biocontrol

Abstract

The present study aimed to examine the capability of Clonostachys rosea isolates as a biological control agent against grapevine trunk diseases pathogens. Five C. rosea and 174 pathogenic fungal strains were isolated from grafted grapevines and subjected to in vitro confrontation tests. Efficient antagonism was observed against Eutypa lata and Phaeomoniella chlamydospora while mycoparasitism was observed to the pathogens of Botryosphaeria dothidea and Diaporthe spp. pathogens in in vitro dual culture assays. The conidia production of the C. rosea isolates were also measured on PDA plates. One isolate (19B/1) with high antagonistic capabilities and efficient conidia production was selected for in planta confrontation tests by mixing its conidia with the soil of Cabernet sauvignon grapevine cuttings artificially infected with B. dothidea, E. lata and P. chlamydospora. The length and/or the incidence of necrotic lesions caused by E. lata and P. chlamydospora at the inoculation point were significantly decreased after a three months incubation in the greenhouse on cuttings planted in soils inoculated with the conidia of strain 19B/1, while symptom incidence and severity were unaffected in the case of the pathogen B. dothidea. Based on the above results, we consider C. rosea a promising biological control agent against some grapevine trunk diseases.

Diversity and antioxidant activity of endophytic fungi isolated from salam (Syzygium polyanthum)

“…This behavior is according to the activation of several mechanisms, such as secreted enzymes to degrade the cell wall, antifungal secondary metabolites production, and plant defense systems induction. Besides having significant biocontrol activity, C. rosea also functions in the biodegradation of plastic waste, biotransformation of bioactive compounds, and source of bioenergy (Sun et al 2020;El-Gendi et al 2022;Geiger et al 2022;Kiruba and Saeid 2022;Kapeua-Ndacnou et al 2023). The findings in this study complement the benefits provided by C. rosea that were previously studied.…”

Microbial Biological Control of Fungi Associated with Grapevine Trunk Diseases: A Review of Strain Diversity, Modes of Action, and Advantages and Limits of Current Strategies

“…These two strains, UCD 8344 and UCD 8189, were inefficient in a dual-culture assay against E. lata. Five endophytic C. rosea strains obtained from grapevine were effective in inhibiting E. lata growth in vitro, without any direct contact [40]. John et al (2005) showed that the treatment of pruning wounds by spores of the saprophyte Fusarium lateritium reduced the recovery of E. lata when the antagonist was applied at least 1 day before the pathogen in a glasshouse assay, as well as on 16 year old healthy vineyards of the cultivar Cabernet Sauvignon in South Australian.…”

Containment of Fusarium culmorum and Its Mycotoxins in Various Biological Systems by Antagonistic Trichoderma and Clonostachys Strains

“…Initial assessment of antagonistic interactions between microorganisms involves the in vitro dual culture bioassay, often using a nutrient-rich PDA medium [ 45 , 46 , 47 , 48 ]. Recently, a bioassay with a dual PDA culture enabled the selection of several Trichoderma strains capable of complete overgrowth and sporulation on the mycelium of several Fusarium species [ 36 , 49 , 50 ].…”