Mycoplasma anseris sp. nov. Found in Geese

Abstract

A mycoplasma designated strain 1219T (T, type strain) was isolated from the phallus of a gander in Hungary. It was assigned to the class Mollicutes, order Mycoplasmatales, on the basis of morphological, cultural, and physical studies. The base composition of its deoxyribonucleic acid was 25 mol% guanine plus cytosine. It was dependent on sterol for growth, and its growth was inhibited by digitonin. The organism was assigned to the genus Mycoplasma since it did not hydrolyze urea and there was no evidence of helical forms. It hydrolyzed arginine, but other biochemical tests were negative. Strain 1219T could not be identified as any of 77 accepted Mycoplasma species by growth inhibition, immunofluorescence, or metabolism inhibition tests and thus appeared to be a new species. We propose the name Mycoplasma anseris for this organism, for which the type strain is strain 1219.Several Mycoplasma and Acholeplasma species have been isolated from geese during the past 10 to 12 years, including M . anatis (12) A. laidlawii, and A. axanthum (17, 20) together with several unclassified strains. Isolations were made, for example, from goose embryo fibroblasts, dead embryos, and the goose respiratory tract.In this paper, we describe the characteristics of a mollicute, strain 1219T (T, type strain), which was isolated from the phallus of a goose in Hungary. We used the methods recommended by the International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes (13) to establish whether this isolate represents a new species. MATERIALS AND METHODSMycoplasma strains. The strains used are shown in Table 1 Media, cultivation, and purification. The media and conditions used to propagate most of these strains were described previously (4), although in Hungary, medium B (8) was used for some species. Attempts to grow M . fastidiosum, M . genitalium, and M . hyopharyngis were unsuccessful. Most morphological and biochemical tests were carried out in both England and Hungary by using the same media used in earlier studies (4, 8,9). Mycoplasma sp. strain 1219T was subcultured routinely at 37°C in a carbon dioxide-rich atmo-

Salpingitis in geese associated withMycoplasmasp. strain 1220

“…Several species of Mollicutes have been detected in geese: M. anatis (Gaillard-Perrin et al, 1983), M. anseris (Bradbury et al, 1988), M. cloacale (Stipkovits et al, 1984b), Mycoplasma gallinarum (Stipkovits et al, 1975), M. gallisepticum (Buntz et al, 1986;Benčina et al, 1987;Levisohn & Kleven, 2000), M. synoviae (Benčina et al, 1987), Mycoplasma imitans (Bradbury et al, 1993) and A. laidlawii and Acholeplasma axanthum (Stipkovits et al, 1975). However, on the basis of serological and biochemical studies, most of our isolates were identified as Mycoplasma sp.…”

“…Agar plates were incubated at 378C for 14 days and examined every 2 days for the appearance of mycoplasma colonies. Mycoplasma isolates were identified by indirect immunofluorescence (Bradbury, 1998) using rabbit antisera against M. anseris (Bradbury et al, 1988), M. cloacale (Bradbury & Forrest, 1984) and Mycoplasma sp. strain 1220 (Stipkovits et al, 1984b).…”

Complete Genome Sequences of Mycoplasma anatis, M. anseris, and M. cloacale Type Strains

“… M. anseris causes airsacculitis, peritonitis, and embryo lethality ( 4 ) and probably has a role in cloaca and phallus inflammation of ganders ( 5 ). The type strain was isolated from a flock with a history of phallus inflammation ( 6 ). The M. cloacale type strain was isolated from a turkey ( 7 ), but this species could be isolated from ducks and geese as well ( 8 , 9 ).…”

Isolation of Mycoplasmas from Clinically Healthy Adult Breeding Geese in Germany

“…species have been culturally isolated from geese over the past 18 years, including M. anatis (GAILLARD-PERRIN et al, 1983), M. cloacale (BRADBURY et a]., 1987), M . gallinarum (STIPKOVITS et al, 1975), M. anseris (BRADBURY et al, 1988), M . gallisepticum (BUNTZ et al, 1986), M .…”