Mycoplasma arginini TUH-14 membrane lipoproteins induce production of interleukin-1, interleukin-6, and tumor necrosis factor alpha by human monocytes

Herbelin, André; Ruuth, E.; Delorme, Delphine; Michel-Herbelin, Catherine; Praz, Françoise

doi:10.1128/iai.62.10.4690-4694.1994

Cited by 61 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, infections with cell wall-free mycoplasmas may be associated with strong inflammatory reactions. Recently, some reports demonstrated the macrophage-stimulatory activity of mycoplasmas or mycoplasmaderived lipoprotein fractions in vitro (9,16,26), suggesting that mycoplasma lipoproteins may be directly responsible for the induction of cytokines and chemokines. In this study, we used MALP-2, a chemically well defined prototype of a mycoplasma-derived lipopeptide, and investigated in detail the spectrum of inflammatory mediators that were released in response to this compound.…”

Section: Discussionmentioning

confidence: 99%

“…However, also preparations from mycoplasmas can powerfully stimulate macrophages/monocytes (2,6,15,33,34), although these microorganisms lack a cell wall. Recently, several reports demonstrated that crude fractions of lipoproteins derived from different mycoplasma strains showed macrophage-stimulatory activities by inducing the production of proinflammatory cytokines (9,16,26). In a more detailed analysis, the lipopeptide MALP-2 (macrophage-activating lipopeptide 2), recently isolated from a clone of Mycoplasma fermentans, has been shown to induce proinflammatory cytokines as well as nitric oxide release from mouse peritoneal macrophages (23).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Induction of Cytokines and Chemokines in Human Monocytes byMycoplasma fermentans-Derived Lipoprotein MALP-2

et al. 1999

View full text Add to dashboard Cite

Bacterial infections are characterized by strong inflammatory reactions. The responsible mediators are often bacterially derived cell wall molecules, such as lipopolysaccharide or lipoteichoic acids, which typically stimulate monocytes and macrophages to release a wide variety of inflammatory cytokines and chemokines. Mycoplasmas, which lack a cell wall, may also stimulate monocytes very efficiently. This study was performed to identify mycoplasma-induced mediators. We investigated the induction of cytokines and chemokines in human monocytes exposed to the Mycoplasma fermentans-derived membrane component MALP-2 (macrophage-activating lipopeptide 2) by dose response and kinetic analysis. We found a rapid and strong MALP-2-inducible chemokine and cytokine gene expression which was followed by the release of chemokines and cytokines with peak levels after 12 to 20 h. MALP-2 induced the neutrophil-attracting CXC chemokines interleukin-8 (IL-8) and GRO-␣ as well as the mononuclear leukocyte-attracting CC chemokines MCP-1, MIP-1␣, and MIP-1␤. Production of the proinflammatory cytokines tumor necrosis factor alpha and IL-6 started at the same time as chemokine release but required 10-to 100-fold-higher MALP-2 doses. The data show that the mycoplasma-derived lipopeptide MALP-2 represents a potent inducer of chemokines and cytokines which may, by the attraction and activation of neutrophils and mononuclear leukocytes, significantly contribute to the inflammatory response during mycoplasma infection.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Induction of Cytokines and Chemokines in Human Monocytes byMycoplasma fermentans-Derived Lipoprotein MALP-2

et al. 1999

View full text Add to dashboard Cite

show abstract

“…In many aspects, this material resembled the mycoplasma-derived highmolecular-weight material (MDHM) which was isolated from M. fermentans by virtue of its macrophage-stimulatory activity (25) and which turned out to be a mixture of lipopeptides (26). It has been previously observed that mycoplasmas, like other microorganisms, produce components which activate macrophages (10,18,20,38) and that they induce inflammation under various natural or experimental conditions (13,16,19,42). In continuation of previous work on MDHM, a welldefined lipopeptide, named 2-kDa macrophage-activating lipopeptide (MALP-2), was isolated from M. fermentans and purified.…”

mentioning

confidence: 91%

Effect of MALP-2, a Lipopeptide from Mycoplasma fermentans , on Bone Resorption In Vitro

et al. 1999

View full text Add to dashboard Cite

Mycoplasmas may be associated with rheumatoid arthritis in various animal hosts. In humans, mycoplasma arthritis has been recorded in association with hypogammaglobulinemia. Mycoplasma fermentans is one mycoplasma species considered to be involved in causing arthritis. To clarify which mycoplasmal compounds contribute to the inflammatory, bone-destructive processes in arthritis, we used a well-defined lipopeptide, 2-kDa macrophage-activating lipopeptide (MALP-2) from M. fermentans, as an example of a class of macrophageactivating compounds ubiquitous in mycoplasmas, to study its effects on bone resorption. MALP-2 stimulated osteoclast-mediated bone resorption in murine calvaria cultures, with a maximal effect at around 2 nM. Antiinflammatory drugs inhibited MALP-2-mediated bone resorption by about 30%. This finding suggests that MALP-2 stimulates bone resorption partially by stimulating the formation of prostaglandins. Since interleukin-6 (IL-6) stimulates bone resorption, we investigated IL-6 production in cultured calvaria. MALP-2 stimulated the liberation of IL-6, while no tumor necrosis factor was detectable. Additionally, MALP-2 stimulated low levels of NO in calvaria cultures, an effect which was strongly increased in the presence of gamma interferon, causing an inhibition of bone resorption. MALP-2 stimulated the bone-resorbing activity of osteoclasts isolated from long bones of newborn rats and cultured on dentine slices without affecting their number. In bone marrow cultures, MALP-2 inhibited the formation of osteoclasts. It appears that MALP-2 has two opposing effects: it increases the bone resorption in bone tissue by stimulation of mature osteoclasts but inhibits the formation of new ones.

show abstract

“…OMPs of Shigella £exneri induced murine macrophages to release TNF-K and IL-6 [29]. Shiga like toxin [30], cell wall glycoproteins [31] and lipoproteins from a number of bacteria [32,33] released several cytokines from human monocytes, murine macrophages and mouse macrophage cell line. Apart from proteins, there have been reports that bacterial PS induced IL-1 release from murine macrophages [34,35] or other cytokines from human PBMC [28].…”

Section: Discussionmentioning

confidence: 99%

Role of porin of Shigella dysenteriae type 1 in modulation of lipopolysaccharide mediated nitric oxide and interleukin-1 release by murine peritoneal macrophages

Biswas

2000

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

The ability of Shigella dysenteriae type 1 porin to induce the release of nitric oxide (NO) and interleukin-1 (IL-1) from peritoneal macrophages of mouse and to regulate lipopolysaccharide (LPS) and gamma interferon (IFN-gamma) mediated release of the two proinflammatory mediators was investigated. Porin released nitrite when added to macrophage cultures. A maximum of 3.2-fold nitrite release by macrophages was observed with 100 ng ml(-1) of porin. The nitrite release of LPS was enhanced significantly by lower concentrations of porin, whereas the effect of IFN-gamma was enhanced by porin at higher concentrations. Polysaccharide (PS) moiety of LPS stimulated the nitrite release of elicited macrophages by 1.6-fold compared to untreated control. It also enhanced the stimulatory effect of 1 and 10 ng ml(-1) of porin by 1.3-fold. Lipid A (LPA) moiety of LPS did not release nitrite, nor did it increase the porin mediated nitrite production. Porin treated 24 h old macrophage culture supernatants were applied for ConA activated thymocyte proliferation as a measure for determination of IL-1 release. Sixty percent depletion of thymocyte proliferation was observed when the porin treated macrophage supernatants were absorbed with anti-IL-1 antibody. A maximum of 5.5-fold increase of thymocyte proliferation over control was found with 1 and 10 ng ml(-1) of porin. One or 10 ng ml(-1) of porin and LPS augmented the thymocyte growth, 1.5-fold beyond that obtained by porin and 1.8-/1. 7-fold more than that obtained by LPS, alone. Similarly, porin and IFN-gamma co-stimulated the cell growth also. PS enhanced the thymocyte proliferation by 5-fold. It also enhanced the thymocyte growth by co-stimulating 1.4-fold the effect observed by 1 or 10 ng ml(-1) of porin alone. LPA could not participate in the cell proliferating activity nor did it enhance the stimulatory effect of porin. Therefore, both nitrite release and thymocyte proliferation by LPS could be substituted by PS only. The tight association of the two bacterial outer membrane components, porin and LPS, could be a necessary co-signal for boosting the release of the two proinflammatory mediators, namely NO and IL-1, which may be associated with the inflammatory response of the colon during Shigella invasion.

show abstract

Mycoplasma arginini TUH-14 membrane lipoproteins induce production of interleukin-1, interleukin-6, and tumor necrosis factor alpha by human monocytes

Cited by 61 publications

References 26 publications

Induction of Cytokines and Chemokines in Human Monocytes byMycoplasma fermentans-Derived Lipoprotein MALP-2

Induction of Cytokines and Chemokines in Human Monocytes byMycoplasma fermentans-Derived Lipoprotein MALP-2

Effect of MALP-2, a Lipopeptide from Mycoplasma fermentans , on Bone Resorption In Vitro

Role of porin of Shigella dysenteriae type 1 in modulation of lipopolysaccharide mediated nitric oxide and interleukin-1 release by murine peritoneal macrophages

Contact Info

Product

Resources

About