Mycoplasma feriruminatoris sp. nov., a fast growing Mycoplasma species isolated from wild Caprinae

Jores, Joerg; Fischer, Anne; Sirand‐Pugnet, Pascal; Thomann, Andreas; Liebler-Tenorio, Elisabeth M.; Schnee, Christiane; Santana-Cruz, Ivette; Heller, Martin; Frey, Joachim

doi:10.1016/j.syapm.2013.07.005

Cited by 24 publications

(50 citation statements)

References 23 publications

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“…Recently several isolates collected from wild Caprinae, namely 4 Alpine ibex ( Capra ibex ) bred in Berlin zoo as well as one Rocky Mountain goat ( Oreamnos americanus ) in the USA, were tentatively assigned to a new mycoplasma species characterized by unusually rapid growth in vitro (Jores et al, 2013). The generation time of Mycoplasma (M.) feriruminatoris sp.…”

Section: Introductionmentioning

confidence: 99%

“…While the M. agalactiae ibex strains were characterized in detail and revealed the emergence of atypical strains, no further investigations were done with the strains classified as Mmc (Tardy et al, 2012). However, the work by Jores et al (2013) made us aware that the rapid growth capacity of these isolates could indicate a possible misidentification of M. feriruminatoris as Mmc .…”

Section: Introductionmentioning

confidence: 99%

“…Both M. feriruminatoris G5847 type strain and 8756-C13 Rocky Mountain strain were included as controls (Manso-Silvan et al, 2007; Jores et al, 2013). Another strain, namely 283F08, isolated in Italy in 2005 from a severe keratoconjunctivitis case in an Alpine ibex, and identified as an atypical Mmc was also included in the study (Giangaspero et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Mycoplasma feriruminatoris sp. nov. Strains Isolated from Alpine Ibex: A 4th Species in the Mycoplasma mycoides Cluster Hosted by Non-domesticated Ruminants?

et al. 2017

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The genus Mycoplasma, a group of free-living, wall-less prokaryotes includes more than 100 species of which dozens are primary pathogens of humans and domesticated animals. Mycoplasma species isolated from wildlife are rarely investigated but could provide a fuller picture of the evolutionary history and diversity of this genus. In 2013 several isolates from wild Caprinae were tentatively assigned to a new species, Mycoplasma (M.) feriruminatoris sp. nov., characterized by an unusually rapid growth in vitro and close genetic proximity to ruminant pathogenic species. We suspected that atypical isolates recently collected from Alpine ibex in France belonged to this new species. The present study was undertaken to verify this hypothesis and to further characterize the French ibex isolates. Phylogenetic analyses were performed to identify the isolates and position them in trees containing several other mycoplasma species pathogenic to domesticated ruminants. Population diversity was characterized by genomic macrorestriction and by examining the capacity of different strains to produce capsular polysaccharides, a feature now known to vary amongst mycoplasma species pathogenic to ruminants. This is the first report of M. feriruminatoris isolation from Alpine ibex in France. Phylogenetic analyses further suggested that M. feriruminatoris might constitute a 4th species in a genetic cluster that so far contains only important ruminant pathogens, the so-called Mycoplasma mycoides cluster. A PCR assay for specific identification is proposed. These French isolates were not clonal, despite being collected in a restricted region of the Alps, which signifies a considerable diversity of the new species. Strains were able to concomitantly produce two types of capsular polysaccharides, β-(1→6)-galactan and β-(1→6)-glucan, with variation in their respective ratio, a feature never before described in mycoplasmas.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Mycoplasma feriruminatoris sp. nov. Strains Isolated from Alpine Ibex: A 4th Species in the Mycoplasma mycoides Cluster Hosted by Non-domesticated Ruminants?

et al. 2017

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“…It encodes 835 open reading frames (ORFs), 2 rRNA operons, and 30 tRNAs. The genetic content of the IVB14/OD_0535 strain is very similar to the genome sequence of M. feriruminatoris G5847 T (7), whose genome was sequenced and published as a draft (88 contigs) in 2013 (8). Of the 835 coding sequences, 680 have assigned functions based on similarities with related mycoplasma proteins in UniProtKB.…”

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confidence: 76%

Complete Genome Sequence of Mycoplasma feriruminatoris Strain IVB14/OD_0535, Isolated from an Alpine Ibex in a Swiss Zoo

Labroussaa

Thomann

Nicholson

et al. 2020

Microbiol Resour Announc

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Laurent Falquet and Joerg Jores contributed equally as co-last authors.ABSTRACT Mycoplasma feriruminatoris is a fast-growing and genetically tractable mycoplasma species. We sequenced the Swiss strain IVB14/OD_0535, isolated from an Alpine ibex. This strain has a circular genome of 1,027,435 bp with a GϩC content of 24.3%. It encodes 835 open reading frames (ORFs), 2 rRNA operons, and 30 tRNAs.M ycoplasma feriruminatoris is phylogenetically closely related to the Mycoplasma mycoides cluster (1), which encompasses the etiological agents of contagious bovine (CBPP) and caprine pleuropneumonia (CCPP), two of the most severe diseases affecting cattle and goats, respectively. To date, the mode of infection and pathogenicity of M. feriruminatoris toward wild and domestic ruminants are still unclear.M. feriruminatoris strain IVB14/OD_0535 was isolated in 2014 from the liver, spleen, and kidney of a 2-month-old Alpine ibex (Capra ibex) which died of septicemia at the Tierpark Dählhölzli (Bern, Switzerland). The organs were cultured at 37°C on tryptone soy agar (TSA-SB; Becton, Dickinson) supplemented with 5% (vol/vol) sheep blood. Mycoplasma-like colonies were subcultivated on mycoplasma agar (MS; Mycoplasma Experience Ltd.), filter cloned three consecutive times, and identified as Mycoplasma feriruminatoris using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (Microflex LT/SH system; Bruker).Genomic DNA was extracted using phenol-chloroform extraction and isopropanol precipitation (2). The quality and quantity of the genomic DNA were assessed on agarose gel and using the Qubit fluorometer (Invitrogen). High-molecular-weight DNA was sheared in a Covaris g-TUBE (Covaris) to obtain 10-kb fragments. DNA was used to prepare a barcoded SMRTbell library with the PacBio SMRTbell template prep kit v.

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“…This lack of information also extends to new bacterial species isolated from wild ruminants. In fact, the description of novel bacteria from such sources is extremely poor, with only five species described since 2001: 'Mycoplasma feriruminatoris' (Jores et al, 2013), Streptococcus porcorum (Vela et al, 2011a), Streptococcus rupicaprae (Vela et al, 2011b), Mycobacterium caprae (Aranaz et al, 1999(Aranaz et al, , 2003 and Bartonella schoenbuchii (Dehio et al, 2001). During an investigation into the microbiota present in tonsils of Iberian ibexes (Capra pyrenaica hispanica), two Streptococcus-like organisms (DICM07-02790-1C T and DICM07-02658-1A) were isolated from two animals that were hunted on the same day and at the same location (National Game Reserve of Puertos de TortosaBeceite, north-eastern Spain).…”

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confidence: 99%

Streptococcus caprae sp. nov., isolated from Iberian ibex (Capra pyrenaica hispanica)

Vela

Mentaberre

Lavı́n

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Biochemical and molecular genetic studies were performed on a novel Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from tonsil samples of two Iberian ibexes. The micro-organism was identified as a streptococcal species based on its cellular, morphological and biochemical characteristics. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus, but the organism did not correspond to any species of this genus. The nearest phylogenetic relative of the unknown coccus from ibex was Streptococcus porci 2923-03 T (96.6 % 16S rRNA gene sequence similarity). Analysis based on rpoB and sodA gene sequences revealed sequence similarity values lower than 86.0 and 83.8 %, respectively, from the type strains of recognized Streptococcus species. The novel bacterial isolate was distinguished from Streptococcus porci and other Streptococcus species using biochemical tests. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as representing a novel species of the genus Streptococcus, for which the name Streptococcus caprae sp. nov. is proposed. The type strain is DICM07-02790-1C T (5CECT 8872 T 5CCUG 67170 T ).

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Mycoplasma feriruminatoris sp. nov., a fast growing Mycoplasma species isolated from wild Caprinae

Cited by 24 publications

References 23 publications

Identification and Characterization of Mycoplasma feriruminatoris sp. nov. Strains Isolated from Alpine Ibex: A 4th Species in the Mycoplasma mycoides Cluster Hosted by Non-domesticated Ruminants?

Identification and Characterization of Mycoplasma feriruminatoris sp. nov. Strains Isolated from Alpine Ibex: A 4th Species in the Mycoplasma mycoides Cluster Hosted by Non-domesticated Ruminants?

Complete Genome Sequence of Mycoplasma feriruminatoris Strain IVB14/OD_0535, Isolated from an Alpine Ibex in a Swiss Zoo

Streptococcus caprae sp. nov., isolated from Iberian ibex (Capra pyrenaica hispanica)

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