Mycoplasma hyopneumoniae and Mycoplasma flocculare differential domains from orthologous surface proteins induce distinct cellular immune responses in mice

Leal, Fernanda Munhoz Dos Anjos; Virginio, Veridiana Gomes; Martello, Carolina Lumertz; Paes, Jéssica Andrade; Borges, Thiago J.; Jaeger, Natália; Bonorino, Cristina; Ferreira, Henrique Bunselmeyer

doi:10.1016/j.vetmic.2016.05.008

Cited by 13 publications

(3 citation statements)

References 28 publications

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“…That M. dispar elicits a milder cellular response might explain why we found this bacterium in such high abundance in the lungs of healthy calves compared to more pathogenic Mycoplasma. A previous study that compared two genetically similar Mycoplasma species, Mycoplasma hyopneumoniae (a causative agent of porcine enzootic pneumonia) and Mycoplasma flocculare (a regular commensal bacterium in the respiratory tract of swine), found that differences in orthologous surface proteins were associated with distinct immunological responses, potentially affecting bacterial survivability [54]. Whether M. dispar confers some form of protective effect to the host (such as inhibiting Mycoplasma bovis colonization in healthy cattle by competing for adhesion sites) or is simply a common respiratory commensal bacterium remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

et al. 2020

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Background: Bacterial bronchopneumonia (BP) is the leading cause of morbidity and mortality in cattle. The nasopharynx is generally accepted as the primary source of pathogenic bacteria that cause BP. However, it has recently been shown in humans that the oropharynx may act as the primary reservoir for pathogens that reach the lung. The objective was therefore to describe the bacterial microbiota present along the entire cattle respiratory tract to determine which upper respiratory tract (URT) niches may contribute the most to the composition of the lung microbiota. Methods: Seventeen upper and lower respiratory tract locations were sampled from 15 healthy feedlot steer calves. Samples were collected using a combination of swabs, protected specimen brushes, and saline washes. DNA was extracted from each sample and the 16S rRNA gene (V3-V4) was sequenced. Community composition, alphadiversity, and beta-diversity were compared among sampling locations. Results: Microbiota composition differed across sampling locations, with physiologically and anatomically distinct locations showing different relative abundances of 1137 observed sequence variants (SVs). An analysis of similarities showed that the lung was more similar to the nasopharynx (R-statistic = 0.091) than it was to the oropharynx (Rstatistic = 0.709) or any other URT sampling location. Five distinct metacommunities were identified across all samples after clustering at the genus level using Dirichlet multinomial mixtures. This included a metacommunity found primarily in the lung and nasopharynx that was dominated by Mycoplasma. Further clustering at the SV level showed a shared metacommunity between the lung and nasopharynx that was dominated by Mycoplasma dispar. Other metacommunities found in the nostrils, tonsils, and oral microbiotas were dominated by Moraxella, Fusobacterium, and Streptococcus, respectively. Conclusions: The nasopharyngeal bacterial microbiota is most similar to the lung bacterial microbiota in healthy cattle and therefore may serve as the primary source of bacteria to the lung. This finding indicates that the nasopharynx is likely the most important location that should be targeted when doing bovine respiratory microbiota research.

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Section: Discussionmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

et al. 2020

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“…There are, however, differences between Mhyo and Mfloc, such as the absence, in Mfloc, of the glpO gene, associated with the production of hydrogen peroxide by Mhyo and cytotoxic activity (Ferrarini et al, 2018(Ferrarini et al, , 2016. Also, there is the presence of differential domains between orthologs of the P97 family of adhesins and other surface proteins (Dos Anjos Leal et al, 2016).…”

Section: Groupmentioning

confidence: 99%

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Ferreira

Mechler-Dreibi

Sonálio

et al. 2021

Veterinary Microbiology

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“…Moreover, previous comparative phylogenetic and phylogenomic studies provided evidences of the close relationship of M. hyopneumoniae and M. flocculare [7–9], which share most of the known virulence-related genes [10]. The differences between M. hyopneumoniae and M. flocculare include the absence, in M. flocculare , of the glpO gene, related to M. hyopneumoniae hydrogen peroxide generation and cytotoxicity [11,12], and differential domains between orthologs from the P97 family of adhesins and from other surface proteins [13]. However, 90% of M. flocculare predicted surface proteins are shared with M. hyopneumoniae [9], and the observed genomic differences between M. hyopneumoniae strains, and between M. hyopneumoniae and M. flocculare so far do not fully explain their differential phenotypes of virulence/pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics of twoMycoplasma hyopneumoniaestrains andMycoplasma flocculareidentified potential porcine enzootic pneumonia determinants

et al. 2018

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Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia (PEP), M. flocculare is a commensal species. To identify potential PEP determinants and provide novel insights on mycoplasma-host interactions, the whole cell proteomes of two M. hyopneumoniae strains, one pathogenic (7448) and other non-pathogenic (J), and M. flocculare were compared. A cell fractioning approach combined with mass spectrometry (LC-MS/MS) proteomics was used to analyze cytoplasmic and surface-enriched protein fractions. Average detection of ~ 50% of the predicted proteomes of M. hyopneumoniae 7448 and J, and M. flocculare was achieved. Many of the identified proteins were differentially represented in M. hyopneumoniae 7448 in comparison to M. hyopneumoniae J and M. flocculare, including potential PEP determinants, such as adhesins, proteases, and redox-balancing proteins, among others. The LC-MS/MS data also provided experimental validation for several genes previously regarded as hypothetical for all analyzed mycoplasmas, including some coding for proteins bearing virulence-related functional domains. The comprehensive proteome profiling of two M. hyopneumoniae strains and M. flocculare provided tens of novel candidates to PEP determinants or virulence factors, beyond those classically described.

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Mycoplasma hyopneumoniae and Mycoplasma flocculare differential domains from orthologous surface proteins induce distinct cellular immune responses in mice

Cited by 13 publications

References 28 publications

Topography of the respiratory tract bacterial microbiota in cattle

Topography of the respiratory tract bacterial microbiota in cattle

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Comparative proteomics of twoMycoplasma hyopneumoniaestrains andMycoplasma flocculareidentified potential porcine enzootic pneumonia determinants

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