Mycoplasma ovis in captive cervids: Prevalence, molecular characterization and phylogeny

Grazziotin, Ana Laura; Santos, Andrea Pires dos; Guimarães, Ana M. S.; Mohamed, Ahmed; Cubas, Zalmir Silvino; Oliveira, Marcos José de; Santos, Leonilda Correia dos; Moraes, Wanderlei de; Vieira, Rafael Felipe da Costa; Donatti, Lucélia; Filho, Ivan Roque de Barros; Biondo, Alexander Welker; Messick, Joanne B.

doi:10.1016/j.vetmic.2011.05.001

Cited by 40 publications

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“…The phylogenetic tree (Fig. 2) confirmed these relationships showing 3 distinct subclusters within the M. suis cluster; sequence 1 is most closely related to M. ovis of sheep and M. ovis-like of fawn; another distinct cluster is shown for sequence 2, which included Candidatus Mycoplasma erythrocervae, and the third for sequence 3 branched with the unclassified hemoplasma described in the Brazilian captive deer (Grazziotin et al, 2011).…”

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“…'Candidatus Mycoplasma erythrocervae' and 'Candidatus Mycoplasma heamocervae' were identified in free-ranging Cervus nippon from Japan (Watanabe et al, 2010) and a hemoplasma most-closely related to M. ovis but of unknown species classification was identified in captive marsh deer (Blastocerus dichotomus) from Brazil (Grazziotin et al, 2011). Their distinction as new species was based on sequence identities of their 16S rRNA, RNAse P (Watanabe et al, 2010), and 23S rRNA genes (Grazzition et al, 2011).…”

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confidence: 99%

“…For sequencing, a conventional PCR was used to amplify nearly the entire (1,341 base pair [bp]) 16S rRNA gene and the 23S rRNA gene (1,345 bp) of mycoplasmas as previously described (Grazziotin et al, 2011). Amplified products from all reactions were separated by 1.5% gel electrophoresis, stained with ethidium bromide, and photographed under ultraviolet light.…”

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Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations

Grazziotin

Duarte

Szabó

et al. 2011

Journal of Wildlife Diseases

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ABSTRACT:Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. ovis infects populations of free-ranging deer in Brazil. Buffy coat samples from 64 Blastocerus dichotomus from Porto Primavera, 18 Ozotocerus bezoarticus from Pantanal, and 21 O. bezoarticus from Emas National Park were tested. Using a M. ovis PCR protocol to amplify extracted DNA, 46/64 (72%) of deer from Porto Primavera, 10/18 (56%) from Pantanal, and 4/ 21 (19%) from Emas National Park were positive, giving an overall positive rate of 58% for hemoplasma in these wild deer. Sequencing and phylogenetic analysis of the 16S rRNA gene revealed 3 genetically distinct hemoplasmas including M. ovis, 'Candidatus Mycoplasma erythrocervae', and a hemoplasma most closely related to M. ovis. Phylogenetic analysis of the 23S rRNA gene from selected sequences confirmed these relationships.

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Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations

Grazziotin

Duarte

Szabó

et al. 2011

Journal of Wildlife Diseases

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“…Hemoplasmas são encontrados por todo mundo e no Brasil já foram identificados em gatos , cães , gado (Girotto et al, 2012), capivaras (Vieira et al, 2009), leões (Guimaraes et al, 2007) e veados (Grazziotin et al, 2011). Recentemente, o potencial zoonótico dos hemoplasmas foi reportado após a identificação molecular de hemoplasmas em um homem com imunossupressão e outro com exposição frequente a animais infectados por hemoplasmas (dos Santos et al, 2008;Steeret al, 2011;Sykes et al, 2010).…”

Section: Estudo Das Infecções Por Hemoplasmas Em Cães E Gatosunclassified

“…Haemoplasmas are found throughout the world and have previously been identified in cats , dogs , cattle (Girotto et al, 2012), capybaras (Vieira et al, 2009), lions (Guimaraes et al, 2007) and deer (Grazziotin et al, 2011) in Brazil. Recently, the zoonotic potential of haemoplasmas has been reported after the molecular identification of haemoplasmas in immunosuppressed humans and professionals, some with frequent exposure to haemoplasma infected animals (dos Santos et al, 2008;Steer et al, 2011;Sykes et al, 2010).…”

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Estudo das infecções por hemoplasmas em cães e gatos domésticos

Aquino¹

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vii AGRADECIMENTOSMeu maior obrigada a minha mãe, Lourdes, por ser não só mãe, mas também pai, amiga, torcedora, guerreira e porto seguro. Ela é meu principal exemplo de ser humano. Dedico e ofereço a ela todas minhas conquistas.Muito obrigada por ser minha maior incentivadora e, principalmente, por acreditar na minha capacidade, até quando eu mesma não acredito.Obrigada ao meu marido Darien por ser grande exemplo de otimismo, bondade e força, por me apoiar incondicionalmente e acreditar em mim.Obrigada pela vida que decidimos trilhar juntos e por tanto amor! Obrigada vó Diva, minha segunda mãe, pelos cuidados e orações.Elas têm funcionado! Obrigada aos meus irmãos, Eduardo, Marlos e Rovena, por toda oportunidade de aprendizado, por serem exemplos tão diferentes e se preocuparem comigo. Aos meus sobrinhos Guilherme e Felipe, que em tão pouco tempo já conquistaram tanto amor em nossos corações e pelas fofuras diárias! Obrigada pela amizade verdadeira, Patrícia, Bruna e Daniela! E por me ensinarem em todos os momentos de convívio e pelo apoio incondicional a qualquer hora! Obrigada Tatiana por todo apoio moral, técnico e intelectual de sua mente brilhante! Obrigada pela paciência e pelas inúmeras ajudas! Obrigada aos colegas de laboratório por toda ajuda e apoio.Meus agradecimentos a minha orientadora, Giane, pela oportunidade oferecida e pela orientação ao longo de tantos anos. Obrigada por ter acreditado no que eu podia oferecer e pela liberdade que tive ao longo dessa pesquisa.Obrigada à CAPES pelo apoio financeiro para que eu pudesse me dedicar exclusivamente a pesquisa e para que eu realizasse o doutorado viii sanduíche na Universidade de Bristol. Obrigada à FAPDF por financiar a apresentação desse projeto em congressos internacionais.Por fim, sou muito grata ao que o doutorado me proporcionou:conhecimento, descobertas, oportunidades e novos horizontes! Espero que esse conteúdo possa contribuir de alguma forma àqueles que se interessam pelas hemoplasmoses. ' (CMhp) are the most prevalent. The aims of the present study were to determine the occurrence and the phylogeny of haemoplasma species from cats in Brasilia and surrounding cities and from dogs in Nigeria, Africa; and assess weather any correlation existed between haemoplasma infection and haematological abnormalities. Blood samples were collected from 451 cats in different origins of Brasília, DF and from 246 dogs from Jos, Plateau State, Nigeria; submitted to erythrocyte evaluation and DNA extraction for PCR analysis and sequencing. In the cats study, haemoplasmas were found in 13.8% of 432 cats. CMhm was the most prevalent species (in 13.8% of cats), followed by Mhf (11.1%) and CMt (4.4%). Over 80% of haemoplasma-infected cats harboured two or more feline haemoplasma species: 7.1% of cats were coinfected with Mhf/CMhm, 0.4% with CMhm/CMt and 3.9% with Mhf/CMhm/CMt. Male gender was significantly associated with haemoplasma infections. No association was found between qPCR haemoplasma status and haematological variables, however CMhm relative copy nu...

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Hematology of Cervids

Garner¹

2022

Schalm's Veterinary Hematology

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Mycoplasma ovis in captive cervids: Prevalence, molecular characterization and phylogeny

Cited by 40 publications

References 9 publications

Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations

Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations

Estudo das infecções por hemoplasmas em cães e gatos domésticos

Hematology of Cervids

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