Mycoplasmas and bacteria in synovial fluid from patients with arthritis.

Mårdh, Per‐Anders; Nilsson, Fried; Bjelle, A

doi:10.1136/ard.32.4.319

Cited by 37 publications

(19 citation statements)

References 15 publications

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“…Pleural effusions in developed countries are due to malignancy while in developing countries it is mainly due to bacterial infections [9]. Joint fluid is a viscous substance that lubricates joints and many bacteria are responsible for the occurrence of arthritis [6] due to presence of bacterial cell wall fragments and bacterial DNA as indicated from experiments [10,11]. bacteria are characterized by having increased WBC count and protein concentration as well as decreased glucose concentration [9].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Teklehymanot¹,

Legese²,

Desta³

2017

Biol Med

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Background: Infections caused by multidrug resistant (MDR) bacteria remain a public health threat for patients and health care workers. There are scarcity of data on bacterial profiles and their drug susceptibility patterns from body fluids in Ethiopia. Hence, this study aimed at assessing bacterial profiles and their antimicrobial susceptibility patterns (AST) from body fluids at Tikur Anbessa Specialized Hospital (TASH), Addis Ababa, Ethiopia.

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Section: Introductionmentioning

confidence: 99%

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Teklehymanot¹,

Legese²,

Desta³

2017

Biol Med

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“…I feymcntuns were not detected more frequently in tlie sera taken from rheumatoid patients. Negative results have been reported by other investigators (3,5,6,30,31). Similarly the present results are consistent with others (8,9) in that no evidence of a cell-mediated response specific for RA patients was found.…”

Section: Discussionmentioning

confidence: 65%

“…Attempts to implicate mycoplasmas in the etiology of human RA have not been conclusive (2,3). Williams' (4) observations on the isolation of Mycoplasma fermentans from synovial material using a preliminary sucrose gradient fractionation or other techniques have not been confirmed (2,3,5,6). These studies have also failed to find an association between the presence of mycoplasmas or humoral antimycoplasma antibodies and human rheumatoid arthritis (3,5,6).…”

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confidence: 99%

“…Williams' (4) observations on the isolation of Mycoplasma fermentans from synovial material using a preliminary sucrose gradient fractionation or other techniques have not been confirmed (2,3,5,6). These studies have also failed to find an association between the presence of mycoplasmas or humoral antimycoplasma antibodies and human rheumatoid arthritis (3,5,6). Reports that the migration of leukocytes from human rheumatoid arthritis patients is inhibited in the presence of M fermentans (7) have not been confirmed (8,9).…”

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confidence: 99%

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Studies on the infectious etiology of human rheumatoid arthritis

Ward

1975

Arthritis & Rheumatism

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A total of 29 rheumatoid patients and 19 nonrheumatoid patients were tested for evidence of present or past infection b y M pneumoniae, M hominis, M fermentam, M arthritidis, M pulmonis, and M hyorhinis. T h e techniques of lymphocyte transformation, metabolic-inhibiting antibody test, and mycoplasmacidal antibody test indicated no significant difference in the response of rheumatoid as opposed to nonrheumatoid patients.Although a wide variety of immunologic processes are apparent within the joints of patients exhibiting rheumatoid arthritis (RA), the nature of the initiating agent or event appears elusive. An infectious etiology for human rheumatoid arthritis remains an attractive hypothesis despite numerous conflicting and inconclusive results on the isolation of an agent.

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“…The very frequent association of M . fermentans with rheumatoid arthritis reported by Williams, Brostoff and Roitt (1 970) remains largely unconfirmed, although the organism has been isolated from the synovial fluid of patients with rheumatoid and non-rheumatoid arthritis (MArdh et al, 1973). The toxic effect of M .…”

Section: Plates Vi-viimentioning

confidence: 97%

Experimental Infection of the Upper Genital Tract of Female Grivet Monkeys With Mycoplasma Fermentans

Møller¹,

Freundt²,

Black³

et al. 1980

Journal of Medical Microbiology

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PLATES VI-VIIM Y C U P L A S M A represents 2% or less of the mycoplasma flora of the lower urogenital tract of man (Black and Rasmussen, 1968; Mirdh, Nilsson and Bjelle, 1973). It has been isolated on one occasion from an inflamed uterine tube (Freundt, 1953), but the possible significance of this species as an aetiological agent in gynaecological inflammatory disease requires further study. The very frequent association of M . fermentans with rheumatoid arthritis reported by Williams, Brostoff and Roitt (1 970) remains largely unconfirmed, although the organism has been isolated from the synovial fluid of patients with rheumatoid and non-rheumatoid arthritis (MArdh et al., 1973). The toxic effect of M . fermentans membrane fractions, when inoculated intraperitoneally in high concentrations into mice (Gabridge and Murphy, 1971;Gabridge, Yip and Hedges, 1975), provides another impetus for studying the potential pathogencity of this organism.In an earlier study, female grivet monkeys proved suitable as an experimental model for infection with Mycoplasma hominis (Msller et al., 1978; Msller and Freundt, 1979). The present paper describes the experimental production of salpingitis and parametritis by M . fermentans in grivet monkeys, by the same techniques. MATERIALS AND METHODSM . fermentans. Two strains were used. Strain S38 was isolated in 1953 from the uterine tube of a patient suffering from recurring salpingitis (Freundt, 1953). After primary isolation and a few subsequent culture passages, the strain was stored in the lyophilised state at 4°C. Upon revival of the lyophil in 1977, cloning was performed three times and the identity of the strain was confirmed as M. fermentans by growth-inhibition and epi-immunofluorescence tests. Strain D1882 was isolated in this laboratory from the cervix of a 15-year-old girl attending a clinic for sexual guidance; it was cloned once. For preparation of inoculum, the organisms were subcultured twice in a liquid B-medium (Freundt, Ern0 and Lemcke, 1979); the second subculture, in 300 ml of medium, was harvested, in the late log phase of growth, by centrifugation (10 000 r.p.m. for 20 min.) and the pellet of organisms was resuspended in 5 ml of PBS, pH 7.2.The concentrated suspension contained approximately 1 O8 colony-forming units (c.f.u.) per ml.Portions of 1 ml were frozen and stored at -70°C, The number of c.f.u. per ml of the individual lots was redetermined before each experiment.

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Mycoplasmas and bacteria in synovial fluid from patients with arthritis.

Cited by 37 publications

References 15 publications

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Studies on the infectious etiology of human rheumatoid arthritis

Experimental Infection of the Upper Genital Tract of Female Grivet Monkeys With Mycoplasma Fermentans

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