Mycoplasmas of Animals

Stalheim, O. H. V.

doi:10.1016/b978-0-12-161775-2.50031-1

Cited by 8 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isolation of mycoplasmas from clinical specimens was carried out using a Hayflick‐based medium as modified by Friis (1975), and the cultural procedures were carried out as described earlier (Kusiluka et al., 2000). The isolates were cloned and characterized biochemically as previously described (Ernø, 1974; Friis, 1975; Cottew and Yeats, 1978; Stalheim, 1990). Mycoplasma isolates were identified serologically by the disc growth inhibition test (Working Group of the FAO/WHO, Programme on Comparative Mycoplasmology, 1976).…”

Section: Methodsmentioning

confidence: 99%

Molecular Epidemiology of Contagious Bovine Pleuropneumonia in Tanzania Based on Amplified Fragment Length Polymorphism and Pulsed‐field Gel Electrophoresis Analysis

Kusiluka

Ojeniyi

Friis³

et al. 2001

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

The genetic diversity of 60 field strains of Mycoplasma mycoides ssp. mycoides, small colony type (M. mycoides SC), comprising 56 isolates from cattle in Tanzania, one from Kenya, two from Botswana and one from Portugal, as well as the type (PG1T) and vaccine (T1-SR49) strains, was investigated. The strains were analyzed for variations in the EcoRI and Csp6I restriction sites in the genomic DNA using the amplified fragment length polymorphism (AFLP) technique, and variations in the BamHI restriction sites using pulsed-field gel electrophoresis (PFGE). Six AFLP types were detected among the analysed strains. The AFLP profiles of the type and vaccine strains were indistinguishable from each other. Indistinguishable AFLP profiles were found for 55 Tanzanian field strains, one of them isolated in 1990 and the other 54 isolated in 1998/1999, although one strain isolated in 1999 showed a different profile. Strains from different countries revealed different AFLP profiles. Six PFGE types were detected among the analysed strains, with all the 56 Tanzanian field strains displaying indistinguishable PFGE profiles. Strains from different countries revealed different PFGE profiles, and so did the type and vaccine strains. The strong genomic homogeneity among M. mycoides SC strains associated with outbreaks of contagious bovine pleuropneumonia in different regions of Tanzania suggests that the outbreaks of the disease in the 1990-99 period might have been caused by a single epidemic clone. Moreover, this study has demonstrated that AFLP and PFGE are potential tools for molecular epidemiological studies of M. mycoides SC infections.

show abstract

Section: Methodsmentioning

confidence: 99%

Molecular Epidemiology of Contagious Bovine Pleuropneumonia in Tanzania Based on Amplified Fragment Length Polymorphism and Pulsed‐field Gel Electrophoresis Analysis

Kusiluka

Ojeniyi

Friis³

et al. 2001

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

show abstract

“…Considering the role of M. ovipneumoniae in the rapid spread of atypical pneumonia in sheep, there is a need for specific but also sensitive and rapid diagnosis of the infected animals. Mycoplasmas are identified by biochemical and serological testing (Erdağ and Türkaslan 1989; Stalheim 1985; Giangaspero et al 2012). Even though cultures are used for diagnosis in most cases, they are cumbersome and need a long time (Lin et al 2008; Weiser et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey

Kiliç

Kalender

Eroksuz

et al. 2013

Trop Anim Health Prod

View full text Add to dashboard Cite

This study used cultures, polymerase chain reaction (PCR), and immunoperoxidase to examine samples from 216 lungs from sheep and lambs with macroscopic pneumonia lesions for the presence of Mycoplasma species. DNA was extracted from lung tissue samples and broth cultures with the help of a DNA extraction kit and replicated using genus-specific and species-specific primers for mycoplasma. The lung samples were examined by the immunoperoxidase method using hyperimmune Mycoplasma ovipneumoniae serum. The randomly amplified polymorphic DNA (RAPD) test was used for the molecular typing of M. ovipneumoniae isolates. Mycoplasma was isolated in the cultures of 80 (37.03 %) of a total of 216 lung samples. Genus-specific mycoplasma DNA was identified by PCR in 96 (44.44 %) samples in broth cultures and 36 (16.66 %) directly in the lung tissue. Of these 96 cases in which genus-specific identification was made, 57 (59.37 %) were positive for reaction with species-specific primers for M. ovipneumoniae and 31 (32.29 %) for Mycoplasma arginini. The DNA of neither of the latter two species could be identified in the remaining eight samples (8.33 %) where mycoplasma had been identified. As for the immunoperoxidase method, it identified M. ovipneumoniae in 61 of 216 lung samples (28 %). Positive staining was concentrated in the bronchial epithelium cell cytoplasm and cell surface. RAPD analysis resulted in 15 different profiles. Our results suggest that PCR methods could be successfully used in the diagnosis of mycoplasma infections as an alternative to culture method and identifying this agent at the species level.

show abstract

“…Isolation of M. bovis from different specimens was carried out using the modi¢ed Hay£ick medium as previously described [9]. Mycoplasma isolates were identi¢ed by the disc growth inhibition test and immuno£uorescence using rabbit hyperimmune sera raised against the type strain PG45 T [10,11], and were cloned by the single colony technique [12] for use in the AFLP typing.…”

Section: Mycoplasma Strains and Cultural Proceduresmentioning

confidence: 99%

Genetic variations among Mycoplasma bovis strains isolated from Danish cattle

Kusiluka

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

The genetic heterogeneity of Mycoplasma bovis strains isolated in Denmark over a 17-year period was investigated. Forty-two field strains isolated from different geographic locations and specimens, including strains from 21 herds involved in two outbreaks of M. bovis-induced mastitis, and the type strain of M. bovis (PG45 T ) were assayed for variations in the BglII and MfeI restriction sites in the chromosomal DNA by using the amplified fragment length polymorphism (AFLP) fingerprinting technique. The obtained genomic fingerprints consisted of 62^68 AFLP fragments in the size range of 50^500 bp. Among the analyzed strains, 18 different AFLP profiles were detected. The similarity between individual fingerprints, calculated by Dice similarity coefficient, ranged from 0.9 to 1.0. Twenty-five strains, including 23 which were isolated during two outbreaks of M. bovis-induced mastitis which occurred 2 years apart, showed indistinguishable AFLP patterns. More genetic diversity was observed among the recent strains. The similarity of the genotypes of the field strains to that of the M. bovis type strain (PG45 T ) was 97.7%. The results of this study have demonstrated a remarkable genomic homogeneity of Danish strains of M. bovis that were probably epidemiologically related and which have remained stable for a considerable length of time. Furthermore, this study has demonstrated that AFLP can be used for genomic fingerprinting and discrimination of M. bovis strains. ß

show abstract

Mycoplasmas of Animals

Cited by 8 publications

References 28 publications

Molecular Epidemiology of Contagious Bovine Pleuropneumonia in Tanzania Based on Amplified Fragment Length Polymorphism and Pulsed‐field Gel Electrophoresis Analysis

Molecular Epidemiology of Contagious Bovine Pleuropneumonia in Tanzania Based on Amplified Fragment Length Polymorphism and Pulsed‐field Gel Electrophoresis Analysis

Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey

Genetic variations among Mycoplasma bovis strains isolated from Danish cattle

Contact Info

Product

Resources

About